Quantikine Immunoassay Control Set 778 Human Serpin E1/PAI-1

For use with catalog number DSE100
Catalog # Availability Size / Price Qty
QC157
R&D Systems ELISA Controls
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Quantikine Immunoassay Control Set 778 Human Serpin E1/PAI-1 Summary

INTENDED USE & DESCRIPTION

For use as quantitative controls for the determination of cytokine concentrations in biological fluids. Concentrations have been assigned using R&D Systems’ Quantikine® kits. Controls are prepared in a buffered protein solution with preservatives. They contain recombinant human cytokines at low, medium and high concentrations. Controls are supplied lyophilized.

STORAGE & STABILITY

Unreconstituted Controls should be stored at 2-8 °C and are stable for at least 6 months from date of receipt. Depending on the analyte of interest, reconstituted controls may be stable when stored at < -20 °C. Users should evaluate the frozen stability of the controls in their application or discard after use.

REAGENT PREPARATION

Reconstitute each vial with the volume of deionized or distilled water indicated on the product datasheet.

PROCEDURE & EXPECTED VALUES

Controls should be assayed in the same manner as unknown specimens.

The acceptable ranges for the analytes in these controls are printed on the product datasheet. Due to possible variations in techniques and methodologies, it is recommended that each laboratory determine its own target range. Laboratories using other test systems should establish their own acceptable ranges as these assays may produce different values.

TECHNICAL HINTS & LIMITATIONS OF THE PROCEDURE 

• The ranges were determined using R&D Systems’ Quantikine kits. If expected values are not obtained, verify that the lot numbers on the vials correspond with the lot numbers listed above and the correct volume of deionized or distilled water was used for reconstitution of the controls. 

• The results obtained with these controls depend upon several factors associated with methods and instrumentation. Test systems other than those supplied by R&D Systems may result in values that differ from those printed on this product datasheet.

Specifications

Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Applications
ELISA Control
Species
Human

Product Datasheets

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Background: Serpin E1/PAI-1

The human serpin superfamily consists of at least 35 members that target not only serine proteases, but also selected cysteine proteases and non-protease proteins. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. They are also involved in activities that extend beyond their ability to inhibit proteases. For instance, they may also regulate blood pressure, angiogenesis, or act as storage/transport proteins.

Long Name
Plasminogen Activator Inhibitor
Entrez Gene IDs
5054 (Human); 18787 (Mouse)
Alternate Names
Endothelial plasminogen activator inhibitor; Nexin; PAI1; PAI-1; PAI1PAI-1; PAISerpin E1; PLANH1; PLANH1plasminogen activator inhibitor 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1; Serpin E1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 1

FAQs

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Reviews for Quantikine Immunoassay Control Set 778 Human Serpin E1/PAI-1

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Quantikine Immunoassay Control Set 778 Human Serpin E1/PAI-1
By Anonymous on 12/24/2020

RnD Low in wells 3B and 4B
RnD High in wells 11H and 12H


Quantikine Immunoassay Control Set 778 Human Serpin E1/PAI-1
By Anonymous on 12/24/2020

QC low found in wells B3&B4 and QC high found in wells H11&H12. Signal was robust and within expected ranges!


Quantikine Immunoassay Control Set 778 Human Serpin E1/PAI-1
By Anonymous on 12/16/2020

Great QC with results within the given range. The medium QC was replaced with an internal QC. Low is found in wells B3 and B4 (duplicate), and High is located in wells H11 and H12(duplicate). %CV was very low.