Home / IgG2A / Rat Anti-Mouse IgG2A PE-conjugated Antibody

Rat Anti-Mouse IgG2A PE-conjugated Antibody

Catalog #: F0129
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
F0129
Detection of Mouse IgG2APrimary Antibody by Flow Cytometry.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat Anti-Mouse IgG2A PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
The specificity of this antibody was confirmed by direct ELISA on mouse IgG 2A monoclonal antibodies, and it does not cross-react with IgG 1 IgG 2B IgG 3 IgM, IgA, or IgE antibodies. By flow cytometry, this isotype-specific secondary antibody detects primary antibodies of the mouse IgG 2A isotype, and does not detect primary antibodies of mouse IgG 1 IgG 2B or IgG 3 class.
Source
Monoclonal Rat IgG1 Clone # 344701
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Cocktail of mouse IgG 1 and IgG 2A
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Mouse IgG<SUB>2A</SUB>Primary Antibody by Flow Cytometry. View Larger

Detection of Mouse IgG2APrimary Antibody by Flow Cytometry. Human peripheral blood lymphocytes were stained with A) Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # MAB3791, Mouse IgG2A, filled histogram) or isotype control (Catalog # MAB003, open histogram), or B) Mouse Anti-Human CD8a Monoclonal Antibody (Catalog # MAB1509, Mouse IgG2B, filled histogram) or isotype control (Catalog # MAB003, open histogram), followed by PE-conjugated Rat Anti-Mouse IgG2Aisotype-specific secondary antibody (Catalog # F0129). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
    12 months from date of receipt, 2 to 8 degreesC as supplied.

Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels. Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2A Fc
Alternate Names
IgG2A

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Citations for Rat Anti-Mouse IgG2A PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Pyrroloquinoline Quinone Administration Alleviates Allergic Airway Inflammation in Mice by Regulating the JAK-STAT Signaling Pathway
    Authors: Z Min, J Zhou, R Mao, B Cui, Y Cheng, Z Chen
    Mediators of Inflammation, 2022-10-29;2022(0):1267841.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Proinsulin-expressing dendritic cells in type 2 neuropathic diabetic patients with and without foot lesions
    Authors: M Sambataro, L Sambado, E Trevisiol, M Cacciatore, A Furlan, PM Stefani, E Seganfredd, E Durante, S Conte, S Della Bell, A Paccagnell, AP Dei Tos
    FASEB J., 2018-02-12;0(0):fj201701279RR.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Expression of lectin-like transcript-1 in human tissues
    Authors: A Llibre, L Garner, A Partridge, GJ Freeman, P Klenerman, CB Willberg
    F1000Res, 2016-12-29;5(0):2929.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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