Rat CCR6 Antibody

Catalog #: MAB8320R Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB8320R-100
MAB8320R-SP

Detection of CCR6 in HEK293 Human Cell Line Transfected with Rat CCR6 and eGFP by Flow Cytometry

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat CCR6 Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat CCR6 in direct ELISAs.

Source

Recombinant Monoclonal Mouse IgG_2B Clone # 876515R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with rat CCR6
Met1-Met366
Accession # Q5BK58

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CCR6 in HEK293 Human Cell Line Transfected with Rat CCR6 and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with either (A) rat CCR6 or (B) irrelevant protein and eGFP was stained with Mouse Anti-Rat CCR6 Monoclonal Antibody (Catalog # MAB8320R) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG2b isotype control antibody staining (Catalog # MAB0041, data not shown). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR6

CCR6 is an approximately 45 kDa G-protein linked seven transmembrane domain receptor that binds the chemokine CCL20/MIP-3 alpha. Among hematopoietic cells, CCR6 is expressed on B cells, immature dendritic cells, neutrophils, and various T cell subsets (e.g. CD4⁺ T cells, regulatory T cells, effector memory Th17 cells, and g/d T cells). It is upregulated in several cancers, Alzheimer’s brain, and the airways in obstructive pulmonary disease. CCR6 plays an important role in mucosal immunity, IgE mediated allergic reactions, immune cell infiltration into sites of inflammation, and tumor cell invasiveness. It also promotes chemotaxis and tumor angiogenesis in response to beta defensins. Rat CCR6 shares 74% and 87% amino acid sequence identity with human and mouse CCR6, respectively.

Entrez Gene IDs

1235 (Human); 12458 (Mouse); 308163 (Rat)

Alternate Names

BN-1; CC-CKR-6; CCR6; CCR-6; CD196 antigen; CD196; chemokine (C-C motif) receptor 6; CKRL3; CKR-L3; CKR-L3G-protein coupled receptor 29; CKRL3LARC receptor; CMKBR6; CMKBR6STRL22; DRY6; GPR29; GPR29C-C CKR-6; GPRCY4; GPR-CY4; GPRCY4DRY-6; lymphocyte, 22; STRL22