Rat CCR6 PE-conjugated Antibody

Catalog #: FAB8320P Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB8320P-025
FAB8320P-100

Detection of CCR6 in HEK293 Human Cell Line Transfected with Rat CCR6 and eGFP by Flow Cytometry.

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All CCR6 Antibodies

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Rat CCR6 PE-conjugated Antibody Summary

Species Reactivity

Rat

Specificity

Stains rat CCR6 transfectants but not irrelevant transfectants in flow cytometry.

Source

Monoclonal Mouse IgG_2B Clone # 876515

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with rat CCR6
Met1-Met366
Accession # NP_001013163

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CCR6 in HEK293 Human Cell Line Transfected with Rat CCR6 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with rat CCR6 and eGFP (top panel) or irrelevant transfectant (bottom panel) were stained with Mouse Anti-Rat CCR6 PE-conjugated Monoclonal Antibody (Catalog # FAB8320P). Quadrant markers were set based on control antibody staining (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR6

CCR6 is a seven transmembrane-spanning G protein-coupled receptor that binds the chemokine MIP-3 alpha and mediates the chemoattraction of B and T lymphocytes.

Entrez Gene IDs

1235 (Human); 12458 (Mouse); 308163 (Rat)

Alternate Names

BN-1; CC-CKR-6; CCR6; CCR-6; CD196 antigen; CD196; chemokine (C-C motif) receptor 6; CKRL3; CKR-L3; CKR-L3G-protein coupled receptor 29; CKRL3LARC receptor; CMKBR6; CMKBR6STRL22; DRY6; GPR29; GPR29C-C CKR-6; GPRCY4; GPR-CY4; GPRCY4DRY-6; lymphocyte, 22; STRL22