Rat CX3CL1/Fractalkine Chemokine Domain Antibody Summary
Gln25-Gly100
Accession # O55145
Applications
Rat CX3CL1/Fractalkine Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Chemotaxis Induced by CX3CL1/Fractalkine and Neutralization by Rat CX3CL1/Fractalkine Antibody. Recombinant Rat CX3CL1/Fractalkine (Catalog # 536-FR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CX3CL1/Fractalkine (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF537). The ND50 is typically 0.3-1.2 µg/mL.
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CX3CL1/Fractalkine in Mouse Brain. CX3CL1/Fractalkine was detected in perfusion fixed frozen sections of mouse brain (cingulate cortex) using 1.7 µg/mL Goat Anti-Rat CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF537) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CX3CL1/Fractalkine
CX3CL1, also named neurotactin, is a member of the delta chemokine subfamily that contains a novel C-X3-C motif. Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Rat CX3CL1 cDNA encodes a 393 amino acid (aa) residue precursor protein with two alternative (21 aa or 24 aa residue) putative signal peptides, a 74 aa or 76 aa residue globular chemokine domain, a 238 aa residue stalk region rich in Gly, Pro, Ser and Thr and containing degenerate mucin-like repeats, a 19 aa residue transmembrane segment and a 36 aa residue cytoplasmic domain. The extracellular domain of CX3CL1 can potentially be released as a soluble protein by proteolysis at the conserved dibasic motif proximal to the transmembrane region. With the exception of the stalk region, rat CX3CL1 shares a high degree of amino acid sequence homology (83% sequence identity) with human and mouse CX3CL1. CX3CL1 is expressed in various tissues including heart, brain, lung, kidney, skeletal muscle, and testis. In rat brain, CX3CL1 expression was found to be localized principally to neurons. The expression of CX3CL1 was also reported to be up-regulated on activated endothelial cells. Membrane-bound CX3CL1 has been shown to promote adhesion of leukocytes. The soluble chemokine domain of human CX3CL1 was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse CX3CL1 was reported to chemoattract neutrophils and T‑lymphocytes but not monocytes. CX3CR1, previously named V28 or chemokine beta receptor‑like 1, has been found to be a specific receptor for CX3CL1. In addition, US28, a 7TM receptor encoded by human cytomegalovirus that binds multiple CC chemokines, has also been shown to bind CX3CL1 with high-affinity.
- Kledal, T.N. et al. (1998) FEBS Lett. 441:209.
- Combadiere, C. et al. (1998) J. Biol. Chem. 273:23799.
- Harrison, J.L. et al. (1998) Proc. Natl. Acad. Sci. USA 95:10896.
- Rossi, D.L. et al. (1998) Genomics 47:163.
Product Datasheets
Citations for Rat CX3CL1/Fractalkine Chemokine Domain Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Temporal alteration of microglia to microinfarcts in rat brain induced by the vascular occlusion with fluorescent microspheres
Authors: Y Shen, J Cui, S Zhang, Y Wang, J Wang, Y Su, D Xu, Y Liu, Y Guo, W Bai
Frontiers in Cellular Neuroscience, 2022-08-03;16(0):956342.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
MicroRNA-195 prevents hippocampal microglial/macrophage polarization towards the M1 phenotype induced by chronic brain hypoperfusion through regulating CX3CL1/CX3CR1 signaling
Authors: M Mao, Y Xu, XY Zhang, L Yang, XB An, Y Qu, YN Chai, YR Wang, TT Li, J Ai
J Neuroinflammation, 2020-08-20;17(1):244.
Species: Rat
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC -
Cx3cr1-deficiency exacerbates alpha-synuclein-A53T induced neuroinflammation and neurodegeneration in a mouse model of Parkinson's disease
Authors: S Castro-Sán, ÁJ García-Yag, T López-Royo, M Casarejos, JL Lanciego, I Lastres-Be
Glia, 2018-04-06;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Noradrenaline induces CX3CL1 production and release by neurons
Authors: José L M Madrigal
Neuropharmacology, 2016-12-05;114(0):146-155.
Species: Rat
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Astaxanthin Inhibits Expression of Retinal Oxidative Stress and Inflammatory Mediators in Streptozotocin-Induced Diabetic Rats.
Authors: Yeh P, Huang H, Yang C, Yang W, Yang C
PLoS ONE, 2016-01-14;11(1):e0146438.
Species: Rat
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC-P, Western Blot -
Selective activation of microglia facilitates synaptic strength.
Authors: Clark A, Gruber-Schoffnegger D, Drdla-Schutting R, Gerhold K, Malcangio M, Sandkuhler J
J Neurosci, 2015-03-18;35(11):4552-70.
Species: Rat
Sample Types: Whole Cells
Applications: Neutralization -
Interactions between chemokines: regulation of fractalkine/CX3CL1 homeostasis by SDF/CXCL12 in cortical neurons.
Authors: Cook A, Hippensteel R, Shimizu S, Nicolai J, Fatatis A, Meucci O
J. Biol. Chem., 2010-02-02;285(14):10563-71.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Inhibition of spinal microglial cathepsin S for the reversal of neuropathic pain.
Authors: Clark AK, Yip PK, Grist J, Gentry C, Staniland AA, Marchand F, Dehvari M, Wotherspoon G, Winter J, Ullah J, Bevan S, Malcangio M
Proc. Natl. Acad. Sci. U.S.A., 2007-06-05;104(25):10655-60.
Species: Rat
Sample Types: In Vivo, Whole Cells
Applications: ICC, Neutralization
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Results published in:
Article title: Noradrenaline induces CX3CL1 production and release by neurons
Article reference: NP6524
Journal title: Neuropharmacology
Final version published online: 08-Dec-2016
DOI information: 10.1016/j.neuropharm.2016.12.001
Traumatic brain injury site in rat brain
Fractalkine antibody (red) and DAPI (blue)
