Rat CXCL3/CINC-2 alpha / beta Antibody

Catalog #: AF516
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF516
AF516-SP

Chemotaxis Induced by CXCL3/CINC‑2 alpha and Neutral-ization by Rat CXCL3/ CINC‑2 alpha / beta Antibody.

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All CXCL3/GRO gamma/CINC-2/DCIP-1 Antibodies

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Rat CXCL3/CINC-2 alpha / beta Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat CXCL3/CINC-2 alpha / beta in direct ELISAs and Western blots. In direct ELISAs, greater than 50% cross-reactivity with recombinant rat (rr) CINC-1, rrCINC-2 beta, and rrCINC-3 is observed, less than 25% cross-reactivity with recombinant mouse (rm) KC, rmMIP-2, recombinant human (rh) GRO alpha, rhGRO beta, and rhGRO gamma is observed, and less than 10% cross-reactivity with rhIL-8 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant rat CXCL3/CINC-2 alpha / beta
Arg33-Ser100
Accession # Q10746

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Rat CXCL3/CINC-2 alpha (Catalog # 516-CA)
Recombinant Rat CXCL3/CINC-2 beta (Catalog # 517-CB)

Neutralization

Measured by its ability to neutralize CXCL3/CINC-2 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND₅₀) is typically 0.5-2.0 µg/mL in the presence of 0.05 µg/mL Recombinant Rat CXCL3/CINC-2 alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Chemotaxis Induced by CXCL3/CINC‑2 alpha and Neutral-ization by Rat CXCL3/ CINC‑2 alpha / beta Antibody. Recombinant Rat CXCL3/ CINC-2a (Catalog # 516-CA) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL3/ CINC-2a (0.05 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CXCL3/ CINC-2a/ beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF516). The ND₅₀ is typically 0.5-2.0 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL3/GRO gamma/CINC-2/DCIP-1

CINC-2 alpha and -2 beta are mRNA splice variant isoforms that differ by only 3 amino acids at their amino-termini. These proteins are CXC chemokines most closely related to human GROs and mouse MIP-2. They are produced by granulation tissue, activated macrophages and fibroblasts. CINC-2 biological activities are mediated by CXCR1 or CXCR2.

Entrez Gene IDs

2921 (Human); 330122 (Mouse); 171551 (Rat)

Alternate Names

chemokine (C-X-C motif) ligand 3; CINC-2; CINC-2b; C-X-C motif chemokine 3; CXCL3; Dcip1; Gm1960; GRO gamma; GRO3 oncogene; GRO3; GROG; GRO-gamma; GRO-gamma(1-73); Growth-regulated protein gamma; Macrophage inflammatory protein 2-beta; melanoma growth stimulatory activity gamma; MGSA gamma; MIP2B; MIP-2b; MIP2-beta; SCYB3

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Citation for Rat CXCL3/CINC-2 alpha / beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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