Rat CXCR3 Antibody

Catalog #: MAB8109R Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB8109R-100
MAB8109R-SP

Detection of CXCR3 in NS0 Cell Line Transfected with Rat CXCR3 and eGFP by Flow Cytometry.

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All CXCR3 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat CXCR3 Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat CXCR3 in ELISAs.

Source

Recombinant Monoclonal Mouse IgG_2B Clone # 868013R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with rat CXCR3
Accession # Q9J119

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CXCR3 in NS0 Cell Line Transfected with Rat CXCR3 and eGFP by Flow Cytometry. NS0 cell line transfected with (A) rat CXCR3 or (B) irrelevant protein and eGFP was stained with Mouse Anti-Rat CXCR3 Monoclonal Antibody (Catalog # MAB8109R) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041) staining. View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCR3

CXCR3, also known as CD183, is an approximately 41 kDa (predicted) 7-transmembrane spanning receptor for the chemokines CXCL9, CXCL10, and CXCL11. It is expressed on activated T cells, B cells, and NK cells during inflammation. It is additionally upregulated on solid tumor cells, tumor endothelium, and cancer stem cells. CXCR3 plays a role in leukocyte recruitment to sites of inflammation. It also contributes to Th1 biased responses during autoimune diseases rheumatoid arthritis, systemic erythematosis, and type 1 diabetes. Rat CXCR3 shares 86% and 96% amino acid sequence identity with human and mouse CXCR3, respectively.

Entrez Gene IDs

2833 (Human); 12766 (Mouse); 84475 (Rat)

Alternate Names

CD183 antigen; CD183; chemokine (C-X-C motif) receptor 3; chemokine (C-X-C) receptor 3; CKR-L2IP-10 receptor; CMKAR3; C-X-C chemokine receptor type 3; CXCR3; CXC-R3; CXCR-3; G protein-coupled receptor 9CD182; GPR9; GPR9Mig-R; Interferon-inducible protein 10 receptor; IP10 receptor; IP10-R; Mig receptor; MigR