Rat GFR alpha-1/GDNF R alpha-1 Antibody Summary
Asp25-Leu445
Accession # Q62997
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Rat GFR alpha ‑1/GDNF R alpha ‑1 by Western Blot. Western blot shows lysates of rat brain tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Rat GFRa-1/GDNF Ra-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF560) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GFRa-1/GDNF Ra-1 at approximately 52 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
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GFR alpha ‑1/GDNF R alpha ‑1 in Rat Spinal Cord. GFRa-1/GDNF Ra-1 was detected in perfusion fixed frozen sections of rat spinal cord using Goat Anti-Rat GFRa-1/GDNF Ra-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF560) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to spinal cord dorsal horn. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GFR alpha-1/GDNF R alpha-1
Glial cell line-derived growth factor (GDNF), neurturin (NTN) and persephin, distant members of the TGF-beta superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits (GDNF receptor alpha - (GFR alpha -1) or GFR alpha -2). GFR alpha -1 and
‑2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase. Soluble GFR alpha s released enzymatically from the cell surface-associated protein with phosphatidylinositol phospholipase C, as well as recombinantly produced soluble GFR alpha -1, can also bind with high-affinity to GDNF and trigger the activation of Ret tyrosine kinase. Rat GFR alpha -1 cDNA encodes a 468 amino acid (aa) residue protein with an
N‑terminal 24 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, rat GFR alpha -1 has a C-terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Human GFR alpha -1 shares 93% amino acid identity with rat GFR alpha -1. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.
- Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
- Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
- Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
Product Datasheets
Citations for Rat GFR alpha-1/GDNF R alpha-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
31
Citations: Showing 1 - 10
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In vitro spermatogenesis in isolated seminiferous tubules of immature mice
Authors: X Feng, T Matsumura, Y Yamashita, T Sato, K Hashimoto, H Odaka, Y Makino, Y Okada, H Nakamura, H Kimura, T Fujii, T Ogawa
PLoS ONE, 2023-04-06;18(4):e0283773.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
In vitro spermatogenesis in isolated seminiferous tubules of immature mice
Authors: X Feng, T Matsumura, Y Yamashita, T Sato, K Hashimoto, H Odaka, Y Makino, Y Okada, H Nakamura, H Kimura, T Fujii, T Ogawa
PLoS ONE, 2023;18(4):e0283773.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
SOX17-positive rete testis epithelium is required for Sertoli valve formation and normal spermiogenesis in the male mouse
Authors: A Uchida, K Imaimatsu, H Suzuki, X Han, H Ushioda, M Uemura, K Imura-Kish, R Hiramatsu, HM Takase, Y Hirate, A Ogura, M Kanai-Azum, A Kudo, Y Kanai
Nature Communications, 2022-12-21;13(1):7860.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
ERR2 and ERR3 promote the development of gamma motor neuron functional properties required for proprioceptive movement control
Authors: MN Khan, P Cherukuri, F Negro, A Rajput, P Fabrowski, V Bansal, C Lancelin, TI Lee, Y Bian, WP Mayer, T Akay, D Müller, S Bonn, D Farina, T Marquardt
PloS Biology, 2022-12-21;20(12):e3001923.
Species: Human
Sample Types: Whole Cells
Applications: IHC -
Temperature sensitivity of DNA double-strand break repair underpins heat-induced meiotic failure in mouse spermatogenesis
Authors: Hirano K, Nonami Y, Nakamura Y et al.
Communications Biology
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The netrin-1 receptor UNC5C contributes to the homeostasis of undifferentiated spermatogonia in adult mice
Authors: V Barroca, C Racine, L Pays, P Fouchet, M Coureuil, I Allemand
Stem Cell Research, 2022-02-25;60(0):102723.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis.
Authors: Qianxing Z, Lele Y, Ruona S et al.
iScience.
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Cdc42 is required for male germline niche development in mice
Authors: Y Mori, S Takashima, M Kanatsu-Sh, Z Yi, T Shinohara
Cell Reports, 2021-08-17;36(7):109550.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Rubicon prevents autophagic degradation of GATA4 to promote Sertoli cell function
Authors: T Yamamuro, S Nakamura, Y Yamano, T Endo, K Yanagawa, A Tokumura, T Matsumura, K Kobayashi, H Mori, Y Enokidani, G Yoshida, H Imoto, T Kawabata, M Hamasaki, A Kuma, S Kuribayash, K Takezawa, Y Okada, M Ozawa, S Fukuhara, T Shinohara, M Ikawa, T Yoshimori
PloS Genetics, 2021-08-05;17(8):e1009688.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Low retinoic acid levels mediate regionalization of the Sertoli valve in the terminal segment of mouse seminiferous tubules
Authors: K Imura-Kish, A Uchida, N Tsunekawa, H Suzuki, HM Takase, Y Hirate, M Kanai-Azum, R Hiramatsu, M Kurohmaru, Y Kanai
Scientific Reports, 2021-01-13;11(1):1110.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Germ cell depletion in recipient testis has adverse effects on spermatogenesis in orthotopically transplanted testis pieces via retinoic acid insufficiency
Authors: A Tsuchimoto, M Tone, N Ogonuki, M Hada, A Ogura, S Takashima
Sci Rep, 2020-07-01;10(1):10796.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
GDNF and GFRalpha1 Are Required for Proper Integration of Adult-Born Hippocampal Neurons
Authors: A Bonafina, MF Trinchero, AS Ríos, P Bekinschte, AF Schinder, G Paratcha, F Ledda
Cell Rep, 2019-12-24;29(13):4308-4319.e4.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
PLZFposc-KITpos-delineated A1-A4-differentiating spermatogonia by subset and stage detection upon Bouin fixation
Authors: RL Tang, LQ Fan
Asian J. Androl., 2019-05-01;21(3):309-318.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids
Authors: BP Hermann, K Cheng, A Singh, L Roa-De La, KN Mutoji, IC Chen, H Gilderslee, JD Lehle, M Mayo, B Westernstr, NC Law, MJ Oatley, EK Velte, BA Niedenberg, D Fritze, S Silber, CB Geyer, JM Oatley, JR McCarrey
Cell Rep, 2018-11-06;25(6):1650-1667.e8.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
The adult human testis transcriptional cell atlas
Authors: J Guo, EJ Grow, H Mlcochova, GJ Maher, C Lindskog, X Nie, Y Guo, Y Takei, J Yun, L Cai, R Kim, DT Carrell, A Goriely, JM Hotaling, BR Cairns
Cell Res., 2018-10-12;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Prolyl isomerase Pin1 is required sperm production by promoting mitosis progression of spermatogonial stem cells
Authors: A Kurita-Suz, Y Kamo, C Uchida, K Tanemura, K Hara, T Uchida
Biochem. Biophys. Res. Commun., 2018-02-10;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: Immunochromatography -
Advanced immunostaining approaches to study early male germ cell development
Authors: BA Niedenberg, CB Geyer
Stem Cell Res, 2018-02-09;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Aging-related limit of exercise efficacy on motor decline
Authors: JC Arnold, MA Cantu, EA Kasanga, VA Nejtek, EV Papa, N Bugnariu, MF Salvatore
PLoS ONE, 2017-11-27;12(11):e0188538.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Novel combinatorial screening identifies neurotrophic factors for selective classes of motor neurons
Authors: S Schaller, D Buttigieg, A Alory, A Jacquier, M Barad, M Merchant, D Gentien, P de la Gran, G Haase
Proc. Natl. Acad. Sci. U.S.A, 2017-03-07;114(12):E2486-E2493.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Transcriptome analysis reveals transmembrane targets on transplantable midbrain dopamine progenitors.
Authors: Bye C, Jonsson M, Bjorklund A, Parish C, Thompson L
Proc Natl Acad Sci U S A, 2015-03-09;112(15):E1946-55.
Species: Mouse, Rat
Sample Types: Tissue Homogenates, Whole Tissue
Applications: Flow Cytometry, IHC -
PAX7 expression defines germline stem cells in the adult testis.
Authors: Aloisio G, Nakada Y, Saatcioglu H, Pena C, Baker M, Tarnawa E, Mukherjee J, Manjunath H, Bugde A, Sengupta A, Amatruda J, Cuevas I, Hamra F, Castrillon D
J Clin Invest, 2014-08-18;124(9):3929-44.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
LIN28A marks the spermatogonial progenitor population and regulates its cyclic expansion.
Authors: Chakraborty P, Buaas F, Sharma M, Snyder E, de Rooij D, Braun R
Stem Cells, 2014-04-01;32(4):860-73.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Glial cell line-derived neurotrophic factor (GDNF) induces neuritogenesis in the cochlear spiral ganglion via neural cell adhesion molecule (NCAM).
Authors: Euteneuer S, Yang K, Chavez E, Leichtle A, Loers G, Olshansky A, Pak K, Schachner M, Ryan A
Mol Cell Neurosci, 2012-12-20;54(0):30-43.
Species: Rat
Sample Types:
Applications: Western Blot -
Retinoic acid availability drives the asynchronous initiation of spermatogonial differentiation in the mouse.
Authors: Snyder EM, Small C, Griswold MD
Biol. Reprod., 2010-07-21;83(5):783-90.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
The heterogeneity of spermatogonia is revealed by their topology and expression of marker proteins including the germ cell-specific proteins Nanos2 and Nanos3.
Authors: Suzuki H, Sada A, Yoshida S, Saga Y
Dev. Biol., 2009-10-08;336(2):222-31.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Glial cell line-derived neurotrophic factor: an intraovarian factor that enhances oocyte developmental competence in vitro.
Authors: Linher K, Wu D, Li J
Endocrinology, 2007-05-31;148(9):4292-301.
Species: Porcine
Sample Types: Cell Lysates, Whole Cells
Applications: Neutralization, Western Blot -
Astrocyte and muscle-derived secreted factors differentially regulate motoneuron survival.
Authors: Taylor AR, Gifondorwa DJ, Newbern JM, Robinson MB, Strupe JL, Prevette D, Oppenheim RW, Milligan CE
J. Neurosci., 2007-01-17;27(3):634-44.
Species: Chicken
Sample Types: Whole Cells
Applications: Neutralization -
Involvement of GDNF and its receptors in the maturation of the periodontal Ruffini endings.
Authors: Igarashi Y, Aita M, Suzuki A, Nandasena T, Kawano Y, Nozawa-Inoue K, Maeda T
Neurosci. Lett., 2006-12-18;412(3):222-6.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Glial cell line-derived neurotrophic factor family members sensitize nociceptors in vitro and produce thermal hyperalgesia in vivo.
Authors: Malin SA, Molliver DC, Koerber HR, Cornuet P, Frye R, Albers KM, Davis BM
J. Neurosci., 2006-08-16;26(33):8588-99.
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC-Fr -
Nerve growth factor, glial cell line-derived neurotrophic factor and neurturin prevent semaphorin 3A-mediated growth cone collapse in adult sensory neurons.
Authors: Wanigasekara Y, Keast JR
Neuroscience, 2006-07-28;142(2):369-79.
Species: Rat
Sample Types: Whole Cells
Applications: ICC -
Neurotrophin and GDNF family ligands promote survival and alter excitotoxic vulnerability of neurons derived from murine embryonic stem cells.
Authors: Lee CS, Tee LY, Dusenbery S, Takata T, Golden JP, Pierchala BA, Gottlieb DI, Johnson EM, Choi DW, Snider BJ
Exp. Neurol., 2005-01-01;191(1):65-76.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
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IF 1:200 detect gfra1 positive cell in testis
Frozen sections from paraformaldehyde fixed postnatal day 1 mouse testes were cut at 5 microns and affixed to sides. Sections were permealbilized with 0.1% Triton X-100 in PBS, then blocked with 3% BSA in 0.1% Triton X-100 in PBS for 30 min. Sections were then incubated for 1 hour at RT with Goat Anti-Rat GFR alpha 1/GDNF R alpha 1 Antibody (R&D Systems AF560) at 5 µg/ml. After washing 3X with 0.1% Triton X-100 in PBS, sections were incubated with Donkey anti-Goat (H+L) Secondary Antibody, Alexa Fluor 555 conjugate (red; ThermoFisher Scientific A21432) for 1 hour at RT. Phalloidin was used a counterstain (blue) to label F-actin.
