Rat Growth Hormone R/GHR Antibody

Catalog #: MAB1211 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1211
MAB1211-SP

Growth Hormone R/GHR Inhibition of Growth Hormone-dependent Cell Proliferation and Neutralization by Rat Growth Hormone R/GHR Antibody.

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Rat Growth Hormone R/GHR Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat Growth Hormone R/GHR in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human GHR is observed, but no cross-reactivity with recombinant mouse GHR is observed.

Source

Monoclonal Mouse IgG_2A Clone # 198314

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat Growth Hormone R/GHR
Phe19-Arg265
Accession # P16310

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Rat Growth Hormone R/GHR Fc Chimera (Catalog # 1211-GR)
under non-reducing conditions only

Neutralization

Measured by its ability to neutralize Growth Hormone R/GHR-mediated inhibition of proliferation in the Nb2‑11 rat lymphoma cell line. The Neutralization Dose (ND₅₀) is typically 0.9-3.6 µg/mL in the presence of 10 ng/mL Recombinant Rat Growth Hormone R/GHR Fc Chimera and 0.2 ng/mL Recombinant Human Growth Hormone.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Growth Hormone R/GHR Inhibition of Growth Hormone-dependent Cell Proliferation and Neutralization by Rat Growth Hormone R/GHR Antibody. Recombinant Rat Growth Hormone R/GHR Fc Chimera (Catalog # 1211-GR) inhibits Recom-binant Human Growth Hormone (Catalog # 1067-GH) induced prolifer-ation in the Nb2-11 rat lymphoma cell line in a dose-dependent manner (orange line). Inhibition of Recom-binant Human Growth Hormone (0.2 ng/mL) activity elicited by Recom-binant Rat Growth Hormone R/GHR Fc Chimera (10 ng/mL) is neutralized (green line) by increasing concent-rations of Mouse Anti-Rat Growth Hormone R/GHR Mono-clonal Antibody (Catalog # MAB1211). The ND₅₀ is typically 0.9-3.6 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Growth Hormone R/GHR

Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins and somatolactin (1, 2). It is synthesized primarily by somatotropes in the anterior pituitary and is released as an endocrine hormone. Other cells and tissues, including lymphoid tissues, can also produce GH (3). GH is a pleiotropic molecule which can act directly or indirectly via IGF-I, to regulate growth and metabolism as well as enhance T cell survival and thymic functions (1, 2, 4). GH exerts its biological actions by binding to the GH receptor (GHR) that is present in many cell types (1, 2). Rat GHR cDNA encodes a 638 amino acid (aa) residue type I transmembrane protein with a 18 aa signal peptide, a 247 aa extracellular domain, a 24 aa transmembrane domain and a 349 aa cytoplasmic domain. An alternatively spliced 297 aa isoform of rat GHR also exists. This 279 aa variant corresponds to the serum GH-binding protein and is identical in sequence to the extracellular domain of the transmembrane protein up to Glu262 (5). Ligation of GHR by GH has been shown to result in receptor dimerization and activation of the JAK/STAT signaling cascade (6). The soluble GHBP has been shown to interfere with GH signaling by competing with the transmembrane receptor of GH. Alternatively, the GHBP has also been shown to enhance GH action by slowing GH clearance (5, 7).

References