Rat IgG 2B Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
IC013F
Detection of Rat IgG Isotype Control by Flow Cytometry
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Product Details
Citations (4)
FAQs
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Rat IgG 2B Fluorescein-conjugated Antibody Summary

Specificity
Selected for use as a rat IgG 2B isotype control for flow cytometry applications.
Source
Monoclonal Rat IgG2B Clone # 141945
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Detection of Rat IgG Control by Flow Cytometry Mouse thymocytes were stained with Rat Anti-Mouse CCR9 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB2160F, filled histogram) or Rat IgG Fluorescein-conjugated Isotype Control Antibody (Catalog # IC013F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
    12 months from date of receipt, 2 to 8 degreesC as supplied.

Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels. Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2B Fc
Alternate Names
IgG2B

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Citations for Rat IgG 2B Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Oxidized LDL triggers changes in oxidative stress and inflammatory biomarkers in human macrophages
    Authors: OJ Lara-Guzmá, Á Gil-Izquie, S Medina, E Osorio, R Álvarez-Qu, N Zuluaga, C Oger, JM Galano, T Durand, K Muñoz-Dura
    Redox Biol, 2017-11-22;15(0):1-11.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Identification of a unique hepatocellular carcinoma line, Li-7, with CD13(+) cancer stem cells hierarchy and population change upon its differentiation during culture and effects of sorafenib.
    Authors: Yamada T, Abei M, Danjoh I, Shirota R, Yamashita T, Hyodo I, Nakamura Y
    BMC Cancer, 2015-04-11;15(0):260.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. CXCL9 causes heterologous desensitization of CXCL12-mediated memory T lymphocyte activation.
    Authors: Giegold O, Ogrissek N, Richter C, Schroder M, Herrero San Juan M, Pfeilschifter J, Radeke H
    J Immunol, 2013-02-27;190(7):3696-705.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. TREM-1 promotes survival during septic shock in mice.
    Authors: Gibot S, Massin F, Marcou M, Taylor V, Stidwill R, Wilson P, Singer M, Bellingan G
    Eur. J. Immunol., 2007-02-01;37(2):456-66.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control

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