Summary
Precision
Recovery
Linearity
Data Examples
Product Datasheets
Preparation and Storage
Background
Related Research Areas
Rat IL-4 Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL)
Sensitivity
5 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum)
Specificity
Natural and recombinant rat IL-4
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Rat IL-4 Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IL-4 levels in cell culture supernates and serum. It contains E. coli-expressed recombinant rat IL-4 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant rat IL-4 accurately. Results obtained using natural rat IL-4 showed dose response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat IL-4.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 48.2 | 111 | 721 | 47.7 | 112 | 742 |
| Standard Deviation | 2.5 | 4.9 | 23.6 | 3.9 | 6.1 | 31.8 |
| CV% | 5.2 | 4.4 | 3.3 | 8.2 | 5.4 | 4.3 |
Recovery
The recovery of rat IL-4 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=5) | 110 | 104-117 |
| Serum (n=7) | 94 | 88-101 |
Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of rat IL-4 in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-4
IL-4 (Interleukin-4) is a cytokine that is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma. The type I receptor for IL-4, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R? and the common ? chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R? and IL-13 R?1. The type II receptor also transduces IL-13 mediated signals.
Long Name:
Interleukin 4
Entrez Gene IDs:
3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
Alternate Names:
B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Assay Procedure
Refer to the product Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent
50 µL Standard, Control, or Sample
100 µL Conjugate
100 µL Substrate Solution
100 µL Stop Solution
Citations for Rat IL-4 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
15
Citations: Showing 1 - 10
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Calpeptin may reverse glucocorticoid-resistance of allergic rhinitis associated with cigarette smoke exposure by down-regulating interferon regulatory factor 1
Authors: Zhu, WX;Xi, Y;Li, F;Jiao, WE;Li, ZJ;Chen, SM;Kong, YG;Xu, Y;Deng, YQ;Zuo, JJ;Tao, ZZ;
Heliyon
Species: Rat
Sample Types: Serum
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Renoprotection Induced by Aerobic Training Is Dependent on Nitric Oxide Bioavailability in Obese Zucker Rats
Authors: RVP Neves, HL Corrêa, IV de Sousa N, MK Souza, F Costa, AS Haro, LA Deus, AL Reis, HG Simões, RV Andrade, CO Assumpção, W Stone, J Prestes, EC Vieira, R de Cássia, V Cruzat, TS Rosa
Oxidative Medicine and Cellular Longevity, 2021-09-28;2021(0):3683796.
Species: Rat
Sample Types: Tissue Homogenates
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Silicified collagen scaffold induces semaphorin 3A secretion by sensory nerves to improve in-situ bone regeneration
Authors: YX Ma, K Jiao, QQ Wan, J Li, MY Liu, ZB Zhang, W Qin, KY Wang, YZ Wang, FR Tay, LN Niu
Bioactive materials, 2021-07-27;9(0):475-490.
Species: Rat
Sample Types: Plasma
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The local cytokine and growth factor response to rhBMP-2 after spinal fusion
Authors: JD Koerner, DZ Markova, GD Schroeder, BP Calio, A Shah, CW Brooks, AR Vaccaro, DG Anderson, CK Kepler
Spine J, 2018-03-14;0(0):.
Species: Rat
Sample Types: Tissue Lysates
Applications: ELISA (Standard) -
Age-, Gender-, andin VivoDifferent Doses of Isoproterenol Modifyin VitroAortic Vasoreactivity and Circulating VCAM-1
Authors: B Nieto-Lima, A Cano-Martí, ME Rubio-Ruiz, I Pérez-Torr, V Guarner-La
Front Physiol, 2018-01-24;9(0):20.
Species: Mouse
Sample Types: Plasma
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Topical use and systemic action of green and roasted coffee oils and ground oils in a cutaneous incision model in rats (Rattus norvegicus albinus)
Authors: BG Lania, J Morari, AL Souza, MND Silva, AR de Almeida, G Veira-Dami, SM Alegre, CL César, LA Velloso, ML Cintra, NB Maia, PENF Velho
PLoS ONE, 2017-12-13;12(12):e0188779.
Species: Rat
Sample Types: Serum
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Association of C-Type Lectin Mincle with Fc?RI?? Subunits Leads to Functional Activation of RBL-2H3 Cells through Syk
Authors: C Honjoh, K Chihara, H Yoshiki, S Yamauchi, K Takeuchi, Y Kato, Y Hida, T Ishizuka, K Sada
Sci Rep, 2017-04-10;7(0):46064.
Species: Rat
Sample Types: Cell Culture Supernates
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Transplanted neural stem cells modulate regulatory T, gammadelta T cells and corresponding cytokines after intracerebral hemorrhage in rats.
Authors: Gao L, Lu Q, Huang L, Ruan L, Yang J, Huang W, ZhuGe W, Zhang Y, Fu B, Jin K, ZhuGe Q
Int J Mol Sci, 2014-03-13;15(3):4431-41.
Species: Rat
Sample Types: Serum
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The effects of low-dose ionizing radiation in the activated rat basophilic leukemia (RBL-2H3) mast cells.
Authors: Joo H, Nam S, Yang K, Kim C, Jin Y, Kim J
J Biol Chem, 2012-06-14;287(33):27789-95.
Species: Rat
Sample Types: Cell Culture Supernates
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Comprehensive gene expression profiling of rat lung reveals distinct acute and chronic responses to cigarette smoke inhalation.
Authors: Stevenson CS, Docx C, Webster R, Battram C, Hynx D, Giddings J, Cooper PR, Chakravarty P, Rahman I, Marwick JA, Kirkham PA, Charman C, Richardson DL, Nirmala NR, Whittaker P, Butler K
Am. J. Physiol. Lung Cell Mol. Physiol., 2007-08-24;293(5):L1183-93.
Species: Rat
Sample Types: BALF
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Cellular recruitment and cytokine generation in a rat model of allergic lung inflammation are differentially modulated by progesterone and estradiol.
Authors: de Oliveira AP, Domingos HV, Cavriani G, Damazo AS, Dos Santos Franco AL, Oliani SM, Oliveira-Filho RM, Vargaftig BB, de Lima WT
Am. J. Physiol., Cell Physiol., 2007-07-18;293(3):C1120-8.
Species: Rat
Sample Types: BALF
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Suppression of autoimmune retinal disease by lovastatin does not require Th2 cytokine induction.
Authors: Gegg ME, Harry R, Hankey D, Zambarakji H, Pryce G, Baker D, Adamson P, Calder V, Greenwood J
J. Immunol., 2005-02-15;174(4):2327-35.
Species: Rat
Sample Types: Cell Culture Supernates
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Insulin treatment improves hepatic morphology and function through modulation of hepatic signals after severe trauma.
Authors: Schubert T, Horch RE
Ann. Surg., 2004-08-01;240(2):340-9.
Species: Rat
Sample Types: Tissue Homogenates
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Insulin attenuates the systemic inflammatory response in endotoxemic rats.
Authors: Jeschke MG, Klein D, Bolder U, Einspanier R
Endocrinology, 2004-06-10;145(9):4084-93.
Species: Rat
Sample Types: Tissue Homogenates
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Local anticandidal immune responses in a rat model of vaginal infection by and protection against Candida albicans.
Authors: de Bernardis F, Santoni G, Boccanera M, Spreghini E, Adriani D, Morelli L, Cassone A
Infect. Immun., 2000-06-01;68(6):3297-304.
Species: Rat
Sample Types: Vaginal Fluid
FAQs
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Reviews for Rat IL-4 Quantikine ELISA Kit
Average Rating: 5 (Based on 3 Reviews)
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Rat IL-4 Quantikine ELISA Kit
By Anonymous
on 12/11/2021
Sample Tested: Serum
Rat IL-4 Quantikine ELISA Kit
By Anonymous
on 12/09/2020
Sample Tested: simulated body fluid
Rat IL-4 Quantikine ELISA Kit
By Anonymous
on 03/13/2020
Sample Tested: simulated body fluid
We tested IL-4 levels in a release assay performed in simulated body fluid. The standard curve was excellent and the CV values were all very low. We have used kits from other companies and found RnD systems kits to be superior to all other companies. The kits from RnD systems result in much lower background levels and much lower CV levels, which really helps with consistency and repeatability. The image shows the standard curve and the green outlines the low CV values.
