Rat Jagged 1 Antibody

Name: Rat Jagged 1 Antibody
Brand: R&D Systems
SKU: AF599
Rating: 5 (1 reviews)

Catalog #: AF599
9 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF599
AF599-SP

Differentiation Induced by Jagged 1 and Neutralization by Rat Jagged 1 Antibody.

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Citations (9)

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Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat Jagged 1 Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat Jagged 1 in ELISAs and Western blots. In sandwich ELISAs, approximately 15% cross-reactivity with recombinant human (rh) Jagged 1 is observed, and less than 0.3% cross-reactivity with rhJagged 2 and recombinant mouse Jagged 2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat Jagged 1
Ser32-Asp1068 (Gly57-Arg59 del, Asp63Thr, Arg64Leu, and Val-Arg-Pro-Tyr ins before Lys69)
Accession # Q63722

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Rat Jagged 1 Fc Chimera (Catalog # 599-JG)
under non-reducing conditions only

Flow Cytometry

2.5 µg/10⁶ cells

Rat cortical stem cells

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Rat Jagged 1 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

0.2-0.8 µg/mL

Use in combination with:

Detection Reagent: Rat Jagged 1 Biotinylated Antibody (Catalog # BAF599)

Standard: Recombinant Rat Jagged 1 Fc Chimera Protein, CF (Catalog # 599-JG)

Neutralization

Measured by its ability to neutralize Jagged 1-induced differentiation in the C3H10T1/2 mouse embryonic fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 3-15 µg/mL in the presence of 3 µg/mL Recombinant Rat Jagged 1 Fc Chimera and 150 ng/mL Recombinant Human BMP‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Differentiation Induced by Jagged 1 and Neutralization by Rat Jagged 1 Antibody. Recombinant Rat Jagged 1 Fc Chimera (Catalog # 599-JG) induces differentiation in the C3H10T1/2 mouse embryonic fibroblast cell line in the presence of Recombinant Human BMP-2 (Catalog # 355-BM) in a dose-dependent manner (orange line), as measured by alkaline phosphatase activity. Differentiation elicited by Recombinant Rat Jagged 1 Fc Chimera (3 µg/mL) in the presence of Recombinant Human BMP-2 (150 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF599). The ND₅₀ is typically 3-15 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Jagged 1

Rat Jagged 1 was the first ligand for Notch identified in mammals. Since both the ligands and receptors are transmembrane proteins, direct cell-cell interactions are thought to be required for activating Notch signaling. Jagged 1 is synthesized as a precursor protein that contains a 21 aa signal sequence, a 1048 aa extracellular region, a 25 aa transmembrane (TM) segment and a short, 226 aa cytoplasmic domain. The large extracellular region has a DSL (Delta, Serrate, Lag-2 consensus sequence) domain followed by 16 EGF-like repeats, and a cysteine-rich (CR) region (1). The extracellular region of rJagged 1 binds to multiple Notch receptors on the cell surface as well as in solid phase binding studies. The DSL motif is necessary for binding to Notch receptors and the EGF repeats modulate the affinity of the interaction with Notch receptors (2). Notch signaling is implicated in many developmental processes in a variety of cell types. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation. Some specific areas where Jagged is involved include hematopoiesis, myogenesis, neurogenesis and development of the vasculature (3). For instance soluble, non-transmembrane forms of Jagged 1 influence behavior in fibroblast cells leading to characteristics exhibited by endothelial cells during angiogenesis (4). Soluble Jagged 1 is also capable of maintaining the survival and enhancing the expansion of human stem cells that are capable of reconstituting hematopoietic lineages in vivo (5). Furthermore, Jagged 1 is implicated in human disease: Alagille syndrome, an autosomal dominant disorder characterized by defects in liver, heart, eye, skeletal, craniofacial tissues, and kidney, is caused by mutations in Jagged 1 (6). Depending on cell types and how soluble forms of the ligand are presented, ligand binding can result in activation or inhibition of Notch signaling (7). Rat Jagged 1 shows 98% and 99% aa identity to human and mouse Jagged 1 extracellular domains respectively. Relative to the extracellular region of rat Jagged 2, the aa identity is 58%.

References

Lindsell, C.E. et al. (1995) Cell 80:909.
Shimizu, K. et al. (1999) J. Biol. Chem. 274:32961.
Lewis, J. (1998) Stem Cell & Dev. Biol. 9:583.
Small, D. et al. (2001) J. Biol. Chem. 276:32022.
Karanu, F. et al. (2000) J. Exp. Med. 192:1365.
Joutel, A. and E. Tounier-Lasserve (1998) Stem Cell & Dev. Biol. 9:619.
Hicks, C. et al. (2002) J Neurosci. Res. 68:655.

Entrez Gene IDs

182 (Human); 16449 (Mouse); 29146 (Rat)

Alternate Names

AGS; AHDMGC104644; Alagille syndrome; AWS; CD339 antigen; CD339; HJ1; JAG1; Jagged 1; Jagged1; JAGL1; protein jagged-1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Rat Jagged 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Measurement of adhesion and traction of cells at high yield (MATCHY) reveals an energetic ratchet driving nephron condensation
Authors: Liu, J;Prahl, LS;Huang, A;Hughes, AJ;
bioRxiv : the preprint server for biology
Species: Murine polyomavirus strain A3
Sample Types: Whole Tissue
Applications: Immunohistochemistry
?-cell Jagged1 is sufficient but not necessary for islet Notch activity and insulin secretory defects in obese mice
Authors: Suda, N;Bartolomé, A;Liang, J;Son, J;Yagishita, Y;Siebel, C;Accili, D;Ding, H;Pajvani, UB;
Molecular metabolism
Species: Murine polyomavirus strain A3
Sample Types: Whole Tissue
Applications: Immunohistochemistry
The onset of circulation triggers a metabolic switch required for endothelial to hematopoietic transition
Authors: E Azzoni, V Frontera, G Anselmi, C Rode, C James, EM Deltcheva, AS Demian, J Brown, C Barone, A Patelli, JR Harman, M Nicholls, SJ Conway, E Morrissey, SEW Jacobsen, DB Sparrow, AL Harris, T Enver, MFTR de Bruijn
Cell Reports, 2021-12-14;37(11):110103.
Species: Mouse, Transgenic Mouse
Sample Types: Whole Tissue
Applications: IHC
BMP9 signaling promotes the normalization of tumor blood vessels
Authors: C Viallard, C Audiger, N Popovic, N Akla, K Lanthier, I Legault-Na, H Melichar, S Costantino, S Lesage, B Larrivée
Oncogene, 2020-02-10;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Notch signalling defines dorsal root ganglia neuroglial fate choice during early neural crest cell migration
Authors: S Wiszniak, Q Schwarz
BMC Neurosci, 2019-04-29;20(1):21.
Species: Mouse
Sample Types: WholeTissue
Applications: IHC-F
Muscle Satellite Cell Cross-Talk with a Vascular Niche Maintains Quiescence via VEGF and Notch Signaling
Authors: M Verma, Y Asakura, BSR Murakonda, T Pengo, C Latroche, B Chazaud, LK McLoon, A Asakura
Cell Stem Cell, 2018-10-04;23(4):530-543.e9.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
PI3 kinase inhibition improves vascular malformations in mouse models of hereditary haemorrhagic telangiectasia
Nat Commun, 2016-11-29;7(0):13650.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Cadherin-based adhesions in the apical endfoot are required for active Notch signaling to control neurogenesis in vertebrates.
Authors: Hatakeyama J, Wakamatsu Y, Nagafuchi A, Kageyama R, Shigemoto R, Shimamura K
Development, 2014-04-01;141(8):1671-82.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Roles of Pofut1 and O-fucose in mammalian Notch signaling.
Authors: Stahl M, Uemura K, Ge C, Shi S, Tashima Y, Stanley P
J. Biol. Chem., 2008-03-17;283(20):13638-51.
Species: Rat
Sample Types: Whole Cells
Applications: Flow Cytometry