Rat Neuroligin 1/NLGN1 Biotinylated Antibody

Catalog #: BAF4340 Datasheet / COA / SDS

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BAF4340

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Rat Neuroligin 1/NLGN1 Biotinylated Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat Neuroligin 1/NLGN1 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat Neuroligin 1/NLGN1isoform 4
Gln46-Leu696
Accession # Q62765.1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Rat Neuroligin 1/NLGN1

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuroligin 1/NLGN1

Neuroligin 1 (neurexin ligand 1; also NL1) is a 116 kDa member of the type-B carboxyesterase/lipase family of proteins. It is postsynaptically expressed on neurons and initiates excitatory presynapse maturation through binding to a select isoform of beta -neurexin. Mature rat NL1 is 798 amino acids (aa) in length. It is expressed as a type I transmembrane glycoprotein that contains a 652 aa extracellular domain (ECD) (aa 46‑697) and a 125 aa cytoplasmic tail. The ECD possesses a nonfunctional esterase homology domain (aa 54‑612). Rat NL1 has at least three alternate splice forms. One shows a deletion of aa 165‑184 plus aa 298‑306, while the remaining two show deletions of either aa 165‑184, or aa 298‑306, respectively. Rat ECD shares 97% and 93% aa identity with mouse and human NL1 CED, respectively.

Entrez Gene IDs

22871 (Human); 192167 (Mouse); 116647 (Rat)

Alternate Names

EC 3.1.1; EC 3.1.1.1; KIAA1070neuroligin-1; MGC45115; Neuroligin 1; NL1; Nlg; NLGN1