Recombinant Cynomolgus CD2 Fc Chimera Protein, CF

Catalog #: 10213-CD Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10213-CD-050

Recombinant Cynomolgus CD2 Fc Chimera Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus CD2 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey CD2 Fc Chimera (Catalog # 10213-CD) is immobilized at 0.25 μg/mL (100 μL/well), the concentration of Recombinant Human CD58/LFA-3 Fc Chimera (Catalog # 10068-CD) that produces 50% of the optimal binding response is 0.15-0.9 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey CD2 protein

Cynomolgus Monkey CD2 (Lys25-Asp209) Accession # Q6SZ61	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q6SZ61

N-terminal Sequence
Analysis

Lys25

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

48 kDa

SDS-PAGE

60-66 kDa, under reducing conditions

Product Datasheets

10213-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10213-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Cynomolgus CD2 Fc Chimera (Catalog # 10213-CD) is immobilized at 0.25 μg/mL, 100 μL/well, Recombinant Human CD58/LFA-3 Fc Chimera (Catalog # 10068-CD) binds with an ED₅₀ of 0.15-0.9 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Cynomolgus Monkey CD2 Fc Chimera (Catalog # 10213-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-66 kDa and 120-135 kDa, respectively.

Reconstitution Calculator

Background: CD2

T cell surface antigen CD2 is a type I glycoprotein belonging to the Ig superfamily and is expressed on T cells, NK cells, B cells and some antigen presenting cells. CD2 functions as an activator of T lymphocytes and thymocytes (1). Mature human CD2 consists of a 185 amino acid (aa) extracellular domain (ECD) with an Ig-like V-type and an Ig-like C2 type domain, a 26 aa transmembrane segment, and a 116 aa cytoplasmic tail. The extracellular domain is composed of two immunoglobulin-superfamily domains with highly-charged binding regions (2). Within the ECD, Cynomolgus CD2 shares 92% aa sequence identity with human CD2. It interacts with both CD58 and CD59 directly to activate T cells and their adhesion pathways (3). The conformational flexibility of the CD2 molecule affects function through the conformational status of the adhesive ligands (4). Together with PSTPIP1, CD2 works to regulate T cell activation (5). These two proteins colocalize with a second CD2-binding protein to signal immunological synapse formation in T cells (6). CD2 is an adhesion molecule present on the cell surface of T cells, natural killer (NK) cells, and B cells; and its interaction with CD58 on antigen-presenting cells plays an important role in their immune reaction (7). CD2-CD58 interactions play a critical role in the anti-tumor immune response, and restoration of this signaling is an important strategy for anti-tumor therapy (8). Furthermore, CD2-CD58 interactions are pivotal for the activation and function of adaptive natural killer cells in human cytomegalovirus infection (9).

References

Sayre, P. et al. (1987). Proc. Natl. Acad. Sci. 84:2941.
Bodian, D.L. et al. (1994) Structure. 2:755.
Hahn, W.C. et al. (1992). Science. 256:1805.
Yang, J.J. et al. (2001). Curr. Prot. Pept. Sci. 2:1.
Yang, H. and Reinherz, EL. (2006). J. Immunol. 176:5898.
Badour, K. et al. (2003). Immunity. 18:141.
Kingma, D.W. et al. (2002) Cytometry 50:243.
Nishikori, M. et al. (2016) J Clin Cell Immunol 7:406.
Rolle, A. et al. (2016) Eur. J. Immunol. 46:2420.

Entrez Gene IDs

914 (Human); 12481 (Mouse); 102120798 (Cynomolgus Monkey)

Alternate Names

CD2 antigen (p50), sheep red blood cell receptor; CD2 antigen; CD2 molecule; CD2; Erythrocyte receptor; FLJ46032; LFA-2; LFA-3 receptor; lymphocyte-function antigen-2; Rosette receptor; SRBC; T11; T-cell surface antigen CD2; T-cell surface antigen T11/Leu-5