Recombinant Cynomolgus CD96 His-tag Protein, CF

Catalog #: 10478-CD Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10478-CD-050

Recombinant Cynomolgus CD96 His-tag Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus CD96 His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey CD96 His-tag (Catalog # 10478-CD) is immobilized at 1 µg/mL (100 µL/well), Recombinant Recombinant Human CD155/PVR Fc Chimera (Catalog # 9174-CD) binds with an ED₅₀ of 6-36 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey CD96 protein
Lys25-Met503, with a C-terminal 6-His tag

Accession #

XP_005548247.1

N-terminal Sequence
Analysis

Lys25

Predicted Molecular Mass

54 kDa

SDS-PAGE

120-145 kDa, under reducing conditions

Product Datasheets

10478-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10478-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Cynomolgus Monkey CD96 His-tag (10478-CD) is immobilized at 1 μg/mL (100 μL/well), Recombinant Human CD155/PVR Fc Chimera (9174-CD) binds with an ED₅₀ of 6-36 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Cynomolgus CD96 His-tag Protein (10478-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 120-145 kDa.

Reconstitution Calculator

Background: CD96

CD96, also known as Tactile (T cell-activated increased late expression), is a type I transmembrane glycoprotein belonging to the Ig superfamily that shows peak expression 6-9 days after activation of T, NK, and a subpopulation of B cells (1, 2). The extracellular domain (ECD) of CD96 contains three highly N- and O‑glycosylated Ig-like domains, with the two N-terminal domains being V-type and the distal domain a C-type structure (1). Within the mature ECD, cynomolgus CD96 shares 87% and 54% amino acid sequence identity with human and mouse CD96, respectively. In human, a splice variant with a 16 aa deletion in the second V-type domain, called CD96v2, can be expressed (2). CD96 is frequently expressed on acute myeloid leukemia and myelodysplastic stem cells (3, 4). It is also expressed on CD4+ and CD8+ T cells, plus NK cells and select B cells (5). Low expression of adhesive human CD96 is a rare cause of C syndrome, a set of developmental anomalies in cranial bone (trigonocephaly), skin and viscera, demonstrating a role for CD96 in development of these tissues (2, 6). An 80 kDa soluble form is elevated in human serum during chronic hepatitis B (9). The most N-terminal Ig-like domain of human CD96 binds to CD155/PVR (poliovirus receptor), but CD96/CD155 interaction is species-specific (2, 7, 10). On NK cells, co-stimulatory CD96 and DNAM-1 (CD226) are thought to have paired activity with inhibitory TIGIT, all of which bind CD155 and various nectins (11, 12). CD96 can promote NK cell adhesion to, and killing of target cells, including tumors that express CD155 (10, 11).

References

Wang, P.L. et al. (1992) J. Immunol. 148:2600.
Meyer, D. et al. (2009) J. Biol. Chem. 284:2235.
Hosen, N. et al. (2007) Proc. Natl. Acad. Sci. USA 104:11008.
Xie, W. et al. (2010) Cytometry A 77:840.
Eriksson, E. M. et al. (2012) PLOS One 7:e51696.
Kaname, T. et al. (2007) Am. J. Hum. Genet. 81:835.
Seth, S. et al. (2007) Biochem. Biophys. Res. Commun. 364:959.
Protein Accession # BAE32358.
Gong, J. et al. (2008) Clin. Exp. Immunol. 155:207.
Fuchs, A. et al. (2004) J. Immunol. 172:3394.
Stanietsky, N. and O. Mandelboim (2010) FEBS Lett. 584:4895.
Xu, Z. and B. Jin (2010) Cell. Mol. Immunol. 7:11.

Entrez Gene IDs

10225 (Human); 84544 (Mouse); 498079 (Rat); 102121080 (Cynomolgus Monkey)

Alternate Names

CD96 antigenMGC22596; CD96 molecule; CD96; DKFZp667E2122; T cell-activated increased late expression protein; TACTILE; TACTILEincreased late expression; T-cell surface protein tactile