Recombinant Cynomolgus IL-17RA/IL-17R Fc Chimera Protein, CF

Catalog #: 11235-IR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11235-IR-100

Recombinant Cynomolgus IL-17RA/IL-17R Fc Chimera Protein Bioactivity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus IL-17RA/IL-17R Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit IL-17-induced IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.050‑0.500 µg/mL in the presence of 10.0 ng/mL Recombinant Human IL‑17 (Catalog # 7955-IL).

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey IL-17RA/IL-17R protein

Cynomolgus Monkey IL-17RA (Leu33-Trp320) Accession # XP_005568119.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

XP_005568119.1

N-terminal Sequence
Analysis

Leu33

Structure / Form

Disulfide-Linked Homodimer

Predicted Molecular Mass

60 kDa

SDS-PAGE

93-103 kDa, under reducing conditions.

Product Datasheets

11235-IR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11235-IR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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SDS-PAGE

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2 μg/lane of Recombinant Cynomolgus IL-17RA/IL-17R Fc Chimera Protein (Catalog # 11235-IR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 93-103 kDa.

Reconstitution Calculator

Background: IL-17RA/IL-17R

IL-17 R, also known as IL-17 RA, is a 120 kDa type I transmembrane glycoprotein protein that plays a central role in inflammatory responses (1-3). Mature mouse IL‑17 R consists of a 291 amino acid (aa) extracellular domain, a 21 aa transmembrane segment, and a 521 aa cytoplasmic domain (4). The cytoplasmic domain contains a region homologous to the TIR domain of the TLR/IL-1 R family (5). Within the extracellular domain, cynomolgus monkey IL-17RA shares 95% sequence identity with human IL-17RA. While the expression of IL-17 is restricted to activated T cells, IL-17 R exhibits a broad tissue distribution (4). Even in the absence of ligand, IL-17 R exists on the cell surface as a multimer (6). IL-17 R can bind IL-17 but must associate with IL-17 RC to transduce signals (7, 8). Interestingly, human IL-17 R does not appear to form productive complexes with mouse IL-17 RC (8). The IL-17 R can also signal in response to IL-17F (9). IL-17 R ligation promotes T cell activation and the production of IL-6, G-CSF, SCF, and multiple pro‑inflammatory chemokines (4, 7, 9, 10). IL-17A and IL-17F synergize with TNF-alpha in the induction of CXCL1, G-CSF, and IL-6 (9, 11). This effect requires the presence of both TNF RI and TNF RII (9). IL-17 interactions with IL-17 R also inhibit the TNF-alpha induced up-regulation of fibroblast CCL5 and VCAM-1 (11). CCL5 and VCAM-1 induced effects are differentially sensitive to blockade with IL-17 R specific antibodies, suggesting that IL-17 R triggers divergent intracellular signals (11). In vivo, IL‑17 R activity is important for increased generation of neutrophils and their recruitment to sites of inflammation (10, 12, 13). IL-17 R is required for host defense against microbial infection and for the progression of arthritis from inflammation to destructive joint erosion (10, 13).

References

Iwakura, Y. and H. Ishigame (2006) J. Clin. Invest. 116:1218.
Moseley, T.A. et al. (2003) Cytokine Growth Factor Rev. 14:155.
Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
Yao, Z. et al. (1995) Immunity 3:811.
Novatchkova, M. et al. (2003) Trends Biochem. Sci. 28:226.
Kramer, J.M. et al. (2006) J. Immunol. 176:711.
Hymowitz, S.G. et al. (2001) EMBO J. 20:5332.
Toy, D. et al. (2006) J. Immunol. 177:36.
McAllister, F. et al. (2005) J. Immunol. 175:404.
Ye, P. et al. (2001) J. Exp. Med. 194:519.
Schnyder, B. et al. (2005) Cytokine 31:191.
Tan, W. et al. (2006) J. Immunol. 176:6186.
Lubberts, E. et al. (2005) J. Immunol. 175:3360.

Long Name

Interleukin 17 Receptor

Entrez Gene IDs

23765 (Human); 16172 (Mouse); 312679 (Rat); 486759 (Canine); 101925679 (Cynomolgus Monkey)

Alternate Names

CD217 antigen; CD217; Cdw217; CDw217interleukin 17 receptor; hIL-17R; IL-17 R; IL-17 RA; IL-17 receptor A; IL17RA; IL-17RA; IL-17RAMGC10262; IL17Rinterleukin-17 receptor A; interleukin 17 receptor A