Recombinant Cynomolgus Monkey CTLA-4 Fc Chimera Protein, CF

Catalog #: 9336-CT Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9336-CT-200
9336-CT-01M

Recombinant Cynomolgus Monkey CTLA-4 Fc Chimera Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus Monkey CTLA-4 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey CTLA‑4 Fc Chimera is coated at 0.5 µg/mL, 100 μL/well, Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 140-B1) binds with an ED₅₀ of 5-30 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey CTLA-4 protein

Cynomolgus Monkey CTLA-4 (Ala37-Asp161) Accession # XP_005574071	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

XP_005574071

N-terminal Sequence
Analysis

Ala37

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

40 kDa

SDS-PAGE

46-57 kDa, reducing conditions

Product Datasheets

9336-CT

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9336-CT

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 1 mg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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When Recombinant Cynomolgus Monkey CTLA-4 Fc Chimera (Catalog # 9336-CT) is coated at 0.5 µg/mL, 100 µL/well, Recombinant Human B7-1/CD80 Fc Chimera (Catalog # 140-B1) binds with an ED₅₀ of 5-30 ng/mL.

Reconstitution Calculator

Background: CTLA-4

CTLA-4 (cytotoxic T-lymphocyte-4, designated CD152), is a type I transmembrane T cell inhibitory molecule that is a member of the Ig superfamily (1, 2). Cyno CTLA-4 has a 98% homology to human CTLA-4. Human or mouse CTLA-4 cDNA encodes 223 amino acids (aa) including a 35 aa signal sequence, a 126 aa extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane (TM) sequence, and a 41 aa cytoplasmic sequence. It is found as a covalent homodimer of 41-43 kDa (2). Within the ECD, human CTLA-4 shares 98%, 68%, 71% and 83-86% aa sequence identity with cynomolgus, mouse, rat and porcine/bovine/rabbit/feline/canine CTLA-4, respectively. A 174 aa form that lacks TM and cytoplasmic sequences (sCTLA-4) is possibly secreted (3-5). In mouse, an isoform lacking the Ig-like domain has ligand-independent inhibitory activity and is termed liCTLA-4 (6). CD28, which is structurally related to CTLA-4, is constitutively expressed on naïve T cells and promotes T cell activation when engaged by B7-2 on antigen-presenting cells (APC) within the immunological synapse (IS) (1, 7, 8). In contrast, CTLA-4 is recruited from intracellular vesicles to the IS beginning 1-2 days after T cell activation (2, 7, 8). It forms a linear lattice with B7-1 on APC, inducing negative regulatory signals and ending T cell activation (9). Abatacept, a therapeutic human CTLA-4-Ig fusion protein (trade name Orencia), competes with CD28 for B7-1 and B7-2 binding and has been used to antagonize T cell activation in autoimmune conditions and to enhance transplant survival (10). Mice deleted for CTLA-4 show no abnormalities until after birth, but then develop lethal autoimmune reactions due to continued T cell activation and poor control by regulatory T cells, which constitutively express CTLA-4 in wild-type mice and humans (11-13).

References

Harper, K. et al. (1991) J. Immunol. 147:1037.
Teft, W.A. et al. (2006) Annu. Rev. Immunol. 24:65.
Magistrelli, G. et al. (1999) Eur. J. Immunol. 29:3596.
Tector, M. et al. (2009) BMC Immunol. 10:51.
Oaks, M.K. and K.M. Hallett (2000) J. Immunol. 164:5015.
Vijayakrishnan, L. et al. (2004) Immunity 20:563.
Pentcheva-Hoang, T. et al. (2004) Immunity 21:401.
Jansson, A. et al. (2005) J. Immunol 175:1575.
Darlington, P.J. et al. (2005) J. Immunol. 175:996.
Platt, A.M. et al. (2010) J. Immunol. 185:1558.
Wing, K. et al. (2008) Science. 322: 271.
Friedline, R.H. et al. (2009) J. Exp. Med. 206:421.
Jain, N. et al. (2010) Proc. Natl. Acad. Sci. USA. 107:1524.

Long Name

Cytotoxic T-lymphocyte-associated Molecule 4

Entrez Gene IDs

1493 (Human); 12477 (Mouse); 403696 (Canine); 102115124 (Cynomolgus Monkey)

Alternate Names

CD; CD152 antigen; CD152; CD152IDDM12; CD28; celiac disease 3; CELIAC3; CTLA4; CTLA-4; cytotoxic T-lymphocyte antigen 4; cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte-associated protein 4; cytotoxic T-lymphocyte-associated serine esterase-4; GRD4; GSE; ICOS; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4