Recombinant Cynomolgus Monkey IL-18 BPa His-tag Protein, CF

Catalog #: 2523-BP Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
2523-BP-050

Recombinant Cynomolgus Monkey IL-18 BPa His-tag Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus Monkey IL-18 BPa His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit the IL-18-induced response of KG‑1 human acute myelogenous leukemia cells. The ED₅₀ for this effect is 0.015-0.075 μg/mL

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey IL-18 BPa protein
Thr28-Arg207, with a C-terminal 6-His tag

Accession #

XP015290916.1

N-terminal Sequence
Analysis

Thr28

Structure / Form

Monomer

Predicted Molecular Mass

20 kDa

SDS-PAGE

54-60 kDa, reducing conditions

Product Datasheets

2523-BP

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2523-BP

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Cynomolgus Monkey IL‑18 BPa inhibits Recombinant Human IL-18-induced IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells. The ED₅₀ for this effect is 0.015-0.075 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Cynomolgus Monkey IL‑18 BPa was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 54-60 kDa.

Reconstitution Calculator

Background: IL-18 BPa

Cynomolgus Interleukin 18 binding protein (IL-18 BP) is a 20 kDa secreted glycoprotein, which functions as an IL-18 antagonist by binding to IL-18 and blocking its biological activity (1). IL-18 BP bears no amino acid sequence homology to the membrane-associated IL-18 and IL-1 receptor proteins. Human IL-18BP encodes for at least four isoforms by alternative splicing. The IL-18 BP isoform a and c each contains one immunoglobulin (Ig)-like C2-type domain while isoform b and d lack a complete Ig domain. The complete Ig domain has been shown to be essential to the binding and neutralizing properties of the binding proteins (2). Cynomolgus IL-18BPa shares 83% sequence identity with human and 62% with mouse IL-18BPa, respectively. Several poxviruses also encode proteins with sequence similarity to IL-18 BP. Viral IL-18 BPs have been shown to bind and inhibit IL-18 responses and may be involved in modulating host immune responses. The expression of IL-18 BP is markedly upregulated by IFN-gamma, suggesting that IL-18 activity is modulated by a negative feedback mechanism mediated by IL-18 BP (3).

References

Mühl, H. et al. (2000) Biochem. Biophys. Res. Commun. 267:960.
Kim, S-H. et al. (2000) Proc. Nat. Acad. Sci. USA 97:1190.
Calderara, S. et al. (2001) Virology 279:22.

Long Name

Interleukin 18 Binding Protein Isoform a

Entrez Gene IDs

10068 (Human); 84388 (Rat); 107126419 (Cynomolgus Monkey)

Alternate Names

IL18 BPa; IL-18 BPa; IL18BP; IL-18BP; IL18BPa; interleukin 18 binding protein; interleukin-18-binding protein; MC51L-53L-54L homolog gene product; tadekinig-alfa