Recombinant Cynomolgus/Rhesus Macaque CD27 Fc Protein, CF

Catalog #: 9904-CD Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9904-CD-100

Recombinant Cynomolgus/Rhesus Macaque CD27 Fc Protein SDS-PAGE

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus/Rhesus Macaque CD27 Fc Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit CD70-induced IL-8 secretion in HT1080 human fibrosarcoma cells transfected with human CD27. The ED₅₀ for this effect is 0.25-1.5 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived CD27/TNFRSF7 protein

Cynomolgus Monkey CD27 (Ala20-Arg191) Accession # XP_005569963.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

XP_005569963.1

N-terminal Sequence
Analysis

Thr21

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

46 kDa

SDS-PAGE

61-68 kDa, reducing conditions

Product Datasheets

9904-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9904-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE

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2 μg/lane of Recombinant Cynomolgus Monkey CD27 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at kDa and kDa, respectively.

Reconstitution Calculator

Background: CD27/TNFRSF7

CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co‑stimulatory molecule that supports lymphocyte activation and survival (1). Mature human CD27 consists of a 172 amino acid (aa) extracellular domain (ECD) with three TNFR cysteine‑rich repeats, a 21 aa transmembrane segment, and a 48 aa cytoplasmic domain (2). Within the ECD, cynomolgus CD27 shares 94%, 62% and 66% aa sequence identity with human, mouse, and rat CD27, respectively. CD27 is expressed as a disulfide‑linked homodimer that carries N‑linked and O‑linked glycosylation (3, 4). Proteolytic cleavage of CD27 results in the shedding of a 28‑32 kDa fragment of the ECD (4). CD27 is weakly expressed on naïve T cells and NK cells and is up‑regulated upon cell activation (4, 5). It is also up‑regulated on activated germinal center B cells, plasma cells, and a subset of memory B cells (6, 7). CD27 binds to the transmembrane glycoprotein CD27 Ligand/CD70 which is expressed on activated B cells, activated T cells, and dendritic cells (1, 8, 9). This interaction contributes to the activation and survival of CD4⁺ helper T cells (8‑11), CD8⁺ effector T cells (12, 13), memory T cells (10), and NK cells (5, 14). Ligation of CD27 on B cells promotes germinal center formation and the expansion and affinity maturation of memory B cell responses (6, 15).

References

Nolte, M.A. et al. (2009) Immunol. Rev. 229:216.
Camerini, D. et al. (1991) J. Immunol. 147:3165.
van Lier, R.A. et al. (1987) J. Immunol. 139:1589.
Loenen, W.A. et al. (1992) Eur. J. Immunol. 22:447.
Takeda, K. et al. (2000) J. Immunol. 164:1741.
Xiao, Y. et al. (2004) J. Immunol. 172:7432.
Jung, J. et al. (2000) Eur. J. Immunol. 30:2437.
Goodwin, R.G. et al. (1993) Cell 73:447.
Bowman, M.R. et al. (1994) J. Immunol. 152:1756.
Hendriks, J. et al. (2000) Nat. Immunol. 1:433.
van Oosterwijk, M.F. et al. (2007) Int. Immunol. 19:713.
Rowley, T.F. and A. Al Shamkhani (2004) J. Immunol. 172:6039.
Hendriks, J. et al. (2003) J. Exp. Med. 198:1369.
Kelly, J.M. et al. (2002) Nat. Immunol. 3:83.
Raman, V.S. et al. (2003) J. Immunol. 171:5876.

Entrez Gene IDs

939 (Human); 21940 (Mouse); 500318 (Rat); 102139476 (Cynomolgus Monkey)

Alternate Names

CD27 antigen; CD27 molecule; CD27; MGC20393; S152CD27L receptor; T cell activation antigen CD27; T cell activation antigen S152; T14; TNFRSF7; TNFRSF7T-cell activation antigen CD27; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7