Recombinant Cynomolgus/Rhesus NKp30 Fc Chimera Protein, CF

Catalog #: 10947-NK Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10947-NK-050

Recombinant Cynomolgus Monkey/Rhesus Macaque NKp30/NCR3 Fc Chimera Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus/Rhesus NKp30 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human B7-H6 His-tag Protein (Catalog # 9309-B7) is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Cynomolgus Monkey/Rhesus Macaque NKp30/NCR3 Fc Chimera (Catalog # 10947-NK) that produces 50% of the optimal binding response is 4.00-24.0 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived NKp30/NCR3 protein

Cynomolgus Monkey NKp30/NCR3 (Leu19-Gly137) Accession # XP_005553602.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

XP_005553602.1

N-terminal Sequence
Analysis

Leu19 and Ala18

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

40 kDa

SDS-PAGE

50-65 kDa, under reducing condtions

Product Datasheets

10947-NK

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10947-NK

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human B7-H6 His-tag Protein (9309-B7) is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Cynomolgus Monkey/Rhesus Macaque NKp30/NCR3 Fc Chimera (Catalog # 10947-NK) that produces 50% of the optimal binding response is 4.00-24.0 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Cynomolgus Monkey/Rhesus Macaque NKp30/NCR3 Fc Chimera (Catalog # 10947-NK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-65 kDa and 100-130 kDa, respectively.

Reconstitution Calculator

Background: NKp30/NCR3

NKp30, also termed Natural cytotoxicity receptor 3 (NCR3), along with NKp44 and NKp46, constitute a group of receptors termed "Natural Cytotoxicity Receptors" (1). These receptors play a major role in triggering NK-mediated killing of most tumor cell lines. Mature NKp30 consists of an extracellular domain (ECD) with a V‑type Ig-like fold, a single transmembrane region and a short cytoplasmic domain (2). A charged residue in the transmembrane domain of NKp30 is responsible for association with the tyrosine-based activating motif (ITAM)‑bearing accessory protein CD3 zeta (2). The ECD of cynomologus NKp30 shares 93% amino acid sequence identity with the ECD of human NKp30. In human, several NKp30 isoforms arising from alternative splicing are known to exist (3). NKp30 is expressed on both resting and activated NK cells of the CD56dim, CD16+ subset and account for ~85% of NK cells found in peripheral blood and spleen (4). While NKp30 is absent from the CD56bright, CD16- subset that constitutes the majority of NK cells in lymph node and tonsil, its expression is up-regulated in these cells upon IL-2 activation (4). Studies with neutralizing antibodies reveal that NKp30 is partially responsible for triggering lytic activity against several tumor cell types and that it is the main receptor responsible for NK-mediated lysis of immature dendritic cells (2, 5). The B7 family member B7-H6, a tumor cell ligand, has been identified as activating NKp30 (6). Additionally, soluble galectin-3 released from tumor cells was found to bind NKp30 thereby inhibiting NKp30-mediated cytotoxicity (7).

References

Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
Pende, D. et al. (1999) J. Exp. Med. 190:1505.
Pinheiro, P.H. et al. (2020) Br J Pharmacol 177:4563.
Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
Ferlazzo, G. et al. (2002) J. Exp. Med. 195:343.
Brandt, C.S. et al. (2009) J Exp Med. 206:1495.
Kellner, C. et al. (2012) J Immunol. 189:5037.

Entrez Gene IDs

259197 (Human); 294251 (Rat); 102133932 (Cynomolgus Monkey)

Alternate Names

Activating natural killer receptor p30; CD337 antigen; CD337; CD337activating NK-A1 receptor; LY117,1C7NK-p30; lymphocyte antigen 117; natural cytotoxicity triggering receptor 3; Natural killer cell p30-related protein; NCR3; NKp30; NKp30MALS