Recombinant Cynomolgus TNF RII/TNFRSF1B Fc Protein, CF

Catalog #: 10377-TR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10377-TR-050

Recombinant Cynomolgus TNF RII/TNFRSF1B Fc Protein Bioactivity

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All TNF RII/TNFRSF1B Proteins and Enzymes

Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus TNF RII/TNFRSF1B Fc Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED₅₀ for this effect is 0.2-1.2 ng/mL in the presence of 0.25 ng/mL of Recombinant Human TNF-alpha (Catalog # 210-TA).

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey TNF RII/TNFRSF1B protein

Cynomolgus Monkey TNF RII/TNFRSF1B (Leu23-Asp257) Accession # XP_005544817.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

XP_005544817.1

N-terminal Sequence
Analysis

Leu23

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

36 kDa

SDS-PAGE

68-80 kDa, under reducing conditions

Product Datasheets

10377-TR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10377-TR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Cynomolgus Monkey TNF RII/TNFRSF1B Fc Chimera (Catalog # 10377-TR) inhibits Recombinant Human TNF-a (Catalog # 210-TA) mediated cytotoxicity in the L-929 mouse fibroblast cells. The ED₅₀ for this effect is 0.2-1.2 ng/mL in the presence of 0.25 ng/mL of Recombinant Human TNF-alpha.

SDS-PAGE

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2 μg/lane of Recombinant Cynomolgus Monkey TNF RII/TNFRSF1B Fc Chimera (Catalog # 10377-TR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 68-80 kDa and 136-160 kDa, respectively.

Reconstitution Calculator

Background: TNF RII/TNFRSF1B

Tumor Necrosis Factor Receptor II (TNF RII), also known as TNFRSF1B, p75/p80, and CD120b, is a type I transmembrane protein that belongs to the TNF receptor superfamily (1-4). The TNF receptor superfamily is comprised of structurally related receptors that bind to TNF-related ligands and regulate numerous processes such as immune cell activation and apoptosis. Receptors in this superfamily are characterized by the presence of a cysteine-rich region in their extracellular domain (ECD) (1-3, 5). Cynomolgus monkey TNF RII shares 97% sequence identity in the ECD with its human homolog. Several receptors in the TNF superfamily also contain intracellular death domains (DDs) that recruit caspase-interacting proteins to initiate apoptosis upon ligand binding. Those receptors that lack DDs, like TNF RII, bind TNF Receptor-associated Factors, which transduce signals generated by activation of these receptors (6, 7). TNF RII is expressed predominantly on cells of the hematopoietic lineage, such as T and natural killer cells, as well as on endothelial cells, microglia, astrocytes, neurons, oligodendrocytes, cardiac myocytes, and thymocytes (6, 8, 9). TNF RII activation primarily initiates pro-inflammatory and pro-survival responses via NF kappa B-dependent signaling pathways (6, 7). However, under certain conditions, TNF RII signaling can induce apoptosis (8). Soluble TNF RII is believed to inhibit TNF biological activity by binding TNF thereby preventing it from activating membrane TNF receptors (10).

References

Dembic, Z. et al. (1990) Cytokine 2:231.
Kohno, T. et al. (1990) Proc. Natl. Acad. Sci. USA 87:8331.
Lewis, M. et al. (1991) Proc. Natl. Acad. Sci. USA 88:2830.
Loetscher, H. et al. (1990) J. Biol. Chem. 265:20131.
Beltinger, C.P. et al. (1996) Genomics 35:94.
Faustman, D. and M. Davis (2010) Nat. Rev. Drug Discov. 9:482.
Ihnatko, R. and M. Kubeš (2007) Gen. Physiol. Biophys. 26:159.
Mason, A.T. et al. (1995) J. Leukoc. Biol. 58:249.
Speeckaert, M.M. et al. (2012) Am. J. Nephrol. 36:261.
Sennikov, S.V. et al. (2014) Mediators Inflamm. 2014:745909.

Long Name

Tumor Necrosis Factor Receptor II

Entrez Gene IDs

7133 (Human); 21938 (Mouse); 156767 (Rat); 102144224 (Cynomolgus Monkey)

Alternate Names

CD120b antigen; CD120b; Etanercept; p75 TNF receptor; p75TBPII; p75TNFR; soluble TNFR1B variant 1; TNF RII; TNFBRp80 TNF-alpha receptor; TNF-R2; TNFR2TNFR1B; TNF-R75; TNFR80; TNFRII; TNF-RII; TNF-R-II; TNFR-II; TNFRSF1B; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor receptor 2; tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor superfamily, member 1B; Tumor necrosis factor receptor type II