Recombinant Human Activin AC Heterodimer Protein

Name: Recombinant Human Activin AC Heterodimer Protein
Brand: R&D Systems
SKU: 4879-AC
Rating: 5 (1 reviews)

Catalog #: 4879-AC
3 Citations 1 Review Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
4879-AC-010/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
4879-AC-010

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Product Details

Citations (3)

FAQs

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Reviews (1)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Activin AC Heterodimer Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. In a 100 µL reaction mixture containing rhActivin AC at 2 nM and rhActivin RIIB/Fc Chimera dilutions at 0.02-41 nM, the concentration of rhActivin RIIB/Fc Chimera (Catalog # 339-RBB) that produces 50% of the optimal binding response is found to be approximately 0.8-4 nM.

Source

Chinese Hamster Ovary cell line, CHO-derived human Activin AC protein

Human Activin beta A
(Gly311 - Ser426)
Accession # NP_002183

Human Activin beta C (Gly237 - Ser352) Accession # NP_005529
N-terminus	C-terminus

Accession #

NP_002183 ( beta A) & NP_005529 ( beta C)

N-terminal Sequence
Analysis

Gly311 ( beta A subunit) & Gly237 ( beta C subunit)

Structure / Form

Disulfide-linked heterodimer

Predicted Molecular Mass

12.9 kDa ( beta A subunit), 12.5 kDa ( beta C subunit)

SDS-PAGE

23 kDa, reducing conditions

Product Datasheets

4879-AC (with carrier)

Product Datasheet

COA

SDS

4879-AC/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4879-AC

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

4879-AC/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCI.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Activin AC

Activins and inhibins are TGF-beta superfamily proteins that regulate a wide range of processes including mesoderm induction, reproductive system development and function, liver growth and regeneration, wound healing, and inflammation. Activins and inhibins share a variety of beta subunits, while inhibins also possess a unique alpha subunit. There are four human inhibin beta subunits ( beta _A, beta _B, beta _C, and beta _E) and a single inhibin alpha subunit, each of which adopts a cysteine-knot structure (1 - 5). Activin AC is a 23 kDa heterodimer of beta _A and beta _C subunits (6). Human beta _A consists of a 20 amino acid (aa) signal sequence, a 290 aa propeptide, and a 116 aa mature segment, while beta _C consists of an 18 aa signal sequence, a 218 aa propeptide, and a 116 aa mature segment (8, 9). The subunit beta propeptides are required for subunit folding, dimerization, and secretion (10). Mature human beta _A shares 64%, 51%, and 44% aa sequence identity with human beta _B, beta _C, and beta _E, respectively. Sequence identity between human, mouse, and rat is 100% for mature beta _A, and 91% - 93% for mature beta _C. The beta _A and beta _C subunits are both expressed in the liver, pituitary, ovary, testis, and adrenal gland (1, 6, 11). The beta _C subunit may dimerize with beta _A, beta _B or the inhibin alpha subunit (6, 7). Overexpression of beta _C results in increased production of Activin AC as well as a reduction in beta _A expression and activin induced signaling (12, 13). The beta _C subunit modulates activin induced effects in a variety of systems by forming intracellular dimers with the beta _A subunit and impeding the release of Activins A, AB, and B (2, 12). Activins signal through heterodimeric receptor complexes composed of type I (Activin RIA or RIB) and type II (Activin RIIA or RIIB) transmembrane Ser/Thr kinases.

References

Thompson, T.B. et al. (2004) Mol. Cell. Endocrinol. 225:9.
Butler, C.M. et al. (2005) Cytokine Growth Factor Rev. 16:377.
Abe, Y. et al. (2004) Growth Factors 22:105.
Vitt, U.A. et al. (2001) Mol. Endocrinol. 15:681.
Werner, S. and C. Alzheimer (2006) Cytokine Growth Factor Rev. 17:157.
Mellor S.L. et al. (2000) J. Clin. Endocrinol. Metab. 85:4851.
Ushiro, Y. et. al. (2006) J. Reprod. Devel. 52:487.
Mason, A.J. et al. (1986) Biochem. Biophys. Res. Commun. 135:957.
Hötten, G. et al. (1995) Biochem. Biophys. Res. Commun. 206:608.
Gray, A.M. and A.J. Mason (1990) Science 247:1328.
Gold, E.J. et al. (2004) Mol. Cell. Endocrinol. 222:61.
Mellor, S.L. et al. (2003) Endocrinology 144:4410.
Wada, W. et al. (2004) Am. J. Physiol. Endocrinol. Metab. 287:E247.

Alternate Names

Activin AC

Related Research Areas

Citations for Recombinant Human Activin AC Heterodimer Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Activins as Dual Specificity TGF-&beta Family Molecules: SMAD-Activation via Activin- and BMP-Type 1 Receptors
Authors: OE Olsen, H Hella, S Elsaadi, C Jacobi, E Martinez-H, T Holien
Biomolecules, 2020-03-29;10(4):.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture
Activin A more prominently regulates muscle mass in primates than does GDF8
Authors: E Latres, J Mastaitis, W Fury, L Miloscio, J Trejos, J Pangilinan, H Okamoto, K Cavino, E Na, A Papatheodo, T Willer, Y Bai, J Hae Kim, A Rafique, S Jaspers, T Stitt, AJ Murphy, GD Yancopoulo, J Gromada
Nat Commun, 2017-04-28;8(0):15153.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance
Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth.
Authors: Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV
J. Biol. Chem., 2011-07-07;286(34):30034-46.
Species: Human, Mouse
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance