Recombinant Human Angiopoietin-like 4 Protein, CF

Name: Recombinant Human Angiopoietin-like 4 Protein, CF
Brand: R&D Systems
SKU: 4487-AN
Rating: 4.7 (3 reviews)

Catalog #: 4487-AN
13 Citations 3 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
4487-AN-050

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Product Details

Citations (13)

FAQs

Supplemental Products

Reviews (3)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Angiopoietin-like 4 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit lipoprotein lipase activity. Yoshida, K. et al. (2002) J. Lipid Res. 43:1770. The IC₅₀ value under conditions in which Recombinant Human Lipoprotein Lipase/LPL (Catalog # 9888-LL) and p-nitrophenyl butyrate are present in 0.1 M sodium phosphate, 0.15 M NaCl, 0.5% Triton® X-100, pH 7.2, is approximately 0.05-2.0 µg/mL.

Source

Mouse myeloma cell line, NS0-derived human Angiopoietin-like Protein 4/ANGPTL4 protein
Gly26-Ser406 (Lys163Ala & Arg164Ala), with a C-terminal 6-His tag

Accession #

Q9BY76

N-terminal Sequence
Analysis

Gly26

Structure / Form

Oligomer

Predicted Molecular Mass

43.6 kDa (monomer)

SDS-PAGE

50-55 kDa, reducing conditions

Product Datasheets

4487-AN

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4487-AN

Formulation	Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and CHAPS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Angiopoietin-like Protein 4/ANGPTL4

Angiopoietin-like 4 (ANGPTL4), also known as FIAF, FARP, and PGAR, is a 55 kDa glycoprotein secreted by the liver and fat tissue. It is structurally related to the angoipoietins and contains an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain which can be proteolytically separated in vivo (1). Mature human ANGPTL4 shares 26%-30% amino acid (aa) sequence identity with ANGPTL1, 2, 3, 5, 6, and 7. It shares approximately 75% aa sequence identity with mouse and rat ANGPT-L4. The coiled coil domain, which is not glycosylated, mediates the formation of variable sized disulfide-linked oligomers (2). This domain directly inhibits lipoprotein lipase, resulting in increased circulating triglyceride levels (3, 4). In humans, the N-terminal fragment and full length ANGPTL4 physically associate with HDL (4). In mouse, however, full length ANGPTL4 associates with HDL, while the N-terminal fragment associates with LDL (4). Circulating ANGPTL4 is decreased in type II diabetics with a subsequent loss of its normal plasma glucose lowering activity (5). Its expression in adipose tissue is induced by fasting and suppressed by feeding (6). In hypoxic areas, ANGPTL4 is induced in both vascular endothelial cells and tumor cells (7, 8). The N-terminal fragment can function as an angiogenesis inhibitor (7, 8). In contrast, the C-terminal fragment modulates cell adhesion through interactions with heparan sulfate proteoglycans, Integrins beta 1 and beta 5, Vitronectin, and Fibronectin, thereby promoting keratinocyte migration and wound healing (7, 9, 10). ANGPTL4 additionally enhances the survival of hematopoietic and mesenchymal stem cells (11, 12). The expression of an undersialylated form of ANGPTL4 in renal podocytes contributes to proteinuria and nephrotic syndrome (13).

References

Zhu, P. et al. (2012) Biosci. Rep. 32:211.
Ge, H. et al. (2004) J. Biol. Chem. 279:2038.
Sukonina, V. et al. (2006) Proc. Natl. Acad. Sci. USA 103:17450.
Mandard, S. et al. (2006) J. Biol. Chem. 281:934.
Xu, A. et al. (2005) Proc. Natl. Acad. Sci. USA 102:6086.
Kersten, S. et al. (2000) J. Biol. Chem. 275:28488.
Cazes, A. et al. (2006) Circ. Res. 99:1207.
Le Jan, S. et al. (2003) Am. J. Pathol. 162:1521.
Goh, Y.Y. et al. (2010) Am. J. Pathol. 177:2791.
Goh, Y.Y. et al. (2010) J. Biol. Chem. 285:32999.
Blank, U. et al. (2012) Eur. J. Haematol. 89:198.
Hou, M. et al. (2014) PLoS ONE 9:e85808.
Clement, L.C. et al. (2011) Nat. Med. 17:117.

Entrez Gene IDs

51129 (Human); 57875 (Mouse); 362850 (Rat)

Alternate Names

Angiopoietin like Protein 4; angiopoietin-like 4; Angiopoietin-like Protein 4; ANGPTL4; ARP4fasting-induced adipose factor; FIAF; FIAFhepatic angiopoietin-related protein; Hepatic fibrinogen/angiopoietin-related protein; HFARP; HFARPANGPTL2; NL2; peroxisome proliferator-activated receptor (PPAR) gamma inducedangiopoietin-related protein; PGAR; PGARangiopoietin-related protein 4; pp1158; PPARG angiopoietin related protein

Related Research Areas

Citations for Recombinant Human Angiopoietin-like 4 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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Oleic acid-induced metastasis of KRAS/p53-mutant colorectal cancer relies on concurrent KRAS activation and IL-8 expression bypassing EGFR activation
Authors: Shen, CJ;Chan, RH;Lin, BW;Li, NC;Huang, YH;Chang, WC;Chen, BK;
Theranostics
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Angiopoietin-like 4 induces head and neck squamous cell carcinoma cell migration through the NRP1/ABL1/PXN pathway
Authors: Hefni, E;Menon, D;Ma, T;Asiedu, EB;Sultan, A;Meiller, T;Schneider, A;Sodhi, A;Montaner, S;
Cellular signalling
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
ANGPTL4 stabilizes atherosclerotic plaques and modulates the phenotypic transition of vascular smooth muscle cells through KLF4 downregulation
Authors: DI Cho, MJ Ahn, HH Cho, M Cho, JH Jun, BG Kang, SY Lim, SJ Yoo, MR Kim, HS Kim, SJ Lee, LT Dat, C Lee, YS Kim, Y Ahn
Experimental & Molecular Medicine, 2023-02-13;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Overexpressed Neuropilin-1 in Endothelial Cells Promotes Endothelial Permeability through Interaction with ANGPTL4 and VEGF in Kawasaki Disease
Authors: J Huang, S Zhang
Mediators of Inflammation, 2021-08-13;2021(0):9914071.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Decreased ANGPTL4 impairs endometrial angiogenesis during peri-implantation period in patients with recurrent implantation failure
Authors: M Li, J Hu, L Yao, M Gao
J. Cell. Mol. Med., 2020-08-03;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Metformin Mitigates Nickel-Elicited Angiopoietin-Like Protein 4 Expression via HIF-1&alpha for Lung Tumorigenesis
Authors: YT Kang, WC Hsu, CC Ou, HC Tai, HT Hsu, KT Yeh, JL Ko
Int J Mol Sci, 2020-01-17;21(2):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Angiopoietin-like 4 promotes osteosarcoma cell proliferation and migration and stimulates osteoclastogenesis
Authors: T Zhang, A Kastrenopo, Q Larrouture, NA Athanasou, HJ Knowles
BMC Cancer, 2018-05-08;18(1):536.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Hypoxia-induced ANGPTL4 sustains tumour growth and anoikis resistance through different mechanisms in scirrhous gastric cancer cell lines
Authors: K Baba, Y Kitajima, S Miyake, J Nakamura, K Wakiyama, H Sato, K Okuyama, H Kitagawa, T Tanaka, M Hiraki, K Yanagihara, H Noshiro
Sci Rep, 2017-09-11;7(1):11127.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Contact of myeloma cells induces a characteristic transcriptome signature in skeletal precursor cells - Implications for myeloma bone disease
Bone, 2016-08-09;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Involvement of angiopoietin-like 4 in matrix remodeling during chondrogenic differentiation of mesenchymal stem cells.
Authors: Mathieu M, Iampietro M, Chuchana P, Guerit D, Djouad F, Noel D, Jorgensen C
J Biol Chem, 2014-02-06;289(12):8402-12.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Fatty acids bind tightly to the N-terminal domain of angiopoietin-like protein 4 and modulate its interaction with lipoprotein lipase.
Authors: Robal, Terje, Larsson, Mikael, Martin, Miina, Olivecrona, Gunilla, Lookene, Aivar
J Biol Chem, 2012-07-07;287(35):29739-52.
Species: Human
Sample Types: Plasma
Applications: Binding Assay

Hypoxia-inducible factor regulates osteoclast-mediated bone resorption: role of angiopoietin-like 4.
Authors: Knowles HJ, Cleton-Jansen AM, Korsching E, Athanasou NA
FASEB J., 2010-07-28;24(12):4648-59.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Viral G protein-coupled receptor up-regulates Angiopoietin-like 4 promoting angiogenesis and vascular permeability in Kaposi's sarcoma.
Authors: Ma T, Jham BC, Hu J
Proc. Natl. Acad. Sci. U.S.A., 2010-07-26;107(32):14363-8.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay