Recombinant Human Annexin A2 Protein, CF

Catalog #: 9409-AN Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9409-AN-050

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Summary Product Datasheets Carrier Free Reconstitution Calculator Background Related Research Areas

Recombinant Human Annexin A2 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Bioassay data are not available.

Source

E. coli-derived human Annexin A2 protein
Ser2-Asp339, with a C-terminal 6-His tag

Accession #

P07355-1

N-terminal Sequence
Analysis

Ser2

Predicted Molecular Mass

39 kDa

SDS-PAGE

38 kDa, reducing conditions

Product Datasheets

9409-AN

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9409-AN

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS and DTT.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Annexin A2

Annexin A2 (ANXA2), also known as Annexin II and Lipocortin II (LPC2), is a 39 kDa member of the Annexin family. Annexins are calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. The Annexins consist of a unique N-terminal domain followed by a homologous C-terminal core domain containing the phospholipid-binding sites. The C-terminal domain is comprised of four 60-70 aa annexin repeats which form a tightly packed disc known as the annexin domain. Members of the Annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways (1). Annexin A2 is heavily involved in membrane organization and traffic (2). Annexin A2 also functions as an autocrine factor to enhance osteoclast formation and bone resorption and is a major cellular substrate of the tyrosine kinase encoded by the SRC oncogene (3, 4). Human Annexin A2 shares 97% identity with mouse and rat Annexin A2.

References

Gerke, V. et al. (2005) Nat. Rev. Mol. Cell Biol. 6:449.
Hayes, M. et al. (2004). Traffic. 5:571.
Siever, D. A., et al. (1997). ‎Int. J. Biochem. Cell Biol. 29:1219.
Thiel, C. A. et al. (1992). J. Cell Sci. 103:733.

Entrez Gene IDs

302 (Human); 12306 (Mouse); 56611 (Rat)

Alternate Names

Annexin A2; Annexin II; annexin-2; ANX2; ANX2L4; ANX2L4LPC2; ANXA2; CAL1H; Calpactin I heavy chain; calpactin I heavy polypeptide; Calpactin-1 heavy chain; chromobindin 8; chromobindin-8; LIP2; LIP2PAP-IV; Lipocortin II; Lipocortin-2; LPC2D; LPC2DP36; p36; PAP-IV; Placental anticoagulant protein IV; Protein I