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Recombinant Human Apolipoprotein H Protein, CF

Catalog #: 5087-AH
1 Citation Datasheet / COA / SDS
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5087-AH-050
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Recombinant Human Apolipoprotein H Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When recombinant human ApoH is immobilized at 2 μg/mL, 100 µL/well, the concentration of recombinant human LDL R (Catalog # 2148-LD) that produces 50% of the optimal binding response is found to be approximately 0.2 ‑ 0.8 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human Apolipoprotein H/ApoH protein
Met1-Cys345
Accession #
P02749
N-terminal Sequence
Analysis
Gly20
Structure / Form
Monomer
Predicted Molecular Mass
36.3 kDa
SDS-PAGE
57-60 kDa, reducing conditions

Product Datasheets

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5087-AH

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5087-AH

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Apolipoprotein H/ApoH

Apolipoprotein H (ApoH), also known as beta 2‑Glycoprotein I/ beta 2-GPI, is a 50 kDa variably glycosylated member of the complement control superfamily of proteins (1, 2). Mature human ApoH consists of four tandem Sushi/SCR repeats followed by one Sushi-like repeat (3, 4). Mature human ApoH shares 76% and 82% aa sequence identity with mouse and rat ApoH, respectively. Hepatocyte-derived ApoH binds directly to negatively charged phospholipids (5). It circulates as a component of lipoprotein particles and as a lipid-free serum protein (6). ApoH also associates with liposomes and apoptotic cell debris, thereby enabling their renal clearance via Megalin uptake (7, 8). Circulating levels of ApoH are postively correlated with triglyceride-rich lipoprotein (VLDL) components in type II diabetes (9). ApoH inhibits thrombosis by blocking the activation of Coagulation Factor XI but also shows procoagulant activity by inhibiting the activation of Protein C (10, 11). ApoH can be cleaved by Plasmin at Lys317 ‑ Thr318, an action that is enhanced by heparin (12, 13). ApoH cleavage reduces its ability to bind phospholipids and inhibit Factor XI activation but confers the ability to bind Plasminogen (10, 12, 14). Cleaved ApoH also demonstrates antiangiogenic activity, whereas intact ApoH does not (14). The production of antibodies against ApoH is a hallmark of Antiphospholipid Syndrome (APS), an autoimmune disorder that leads to hypercoagulability and recurrent miscarriages (15). ApoH binds to the surface antigen of Hepatitis B Virus and is associated with the development of HBV-induced hepatocellular carcinoma (6, 16).

References
  1. Crook, M.A. et al. (2010) Atherosclerosis 209:32.
  2. Miyakis, S. et al. (2004) Thromb. Res. 114:335.
  3. Steinkasserer, A. et al. (1991) Biochem. J. 277:387.
  4. Lozier, J. et al. (1984) Proc. Natl. Acad. Sci. 81:3640.
  5. Wurm, H. (1984) Int. J. Biochem. 16:511.
  6. Mehdi, H. et al. (1994) J. Virol. 68:2415.
  7. Chonn, A. et al. (1995) J. Biol. Chem. 270:25845.
  8. Moestrup, S.K. et al. (1998) J. Clin. Invest. 102:902.
  9. Castro, A. et al. (2010) Atherosclerosis 209:201.
  10. Shi, T. et al. (2004) Proc. Natl. Acad. Sci. 101:3939.
  11. Mori, T. et al. (1996) Thromb. Haemost. 75:49.
  12. Hunt, J. et al. (1993) Proc. Natl. Acad. Sci. 90:2141.
  13. Guerin, J. et al. (2002) J. Biol. Chem. 277:2644.
  14. Sakai, T. et al. (2007) Am. J. Pathol. 171:1659.
  15. Adams, M. (2008) Semin. Thromb. Haemost. 34:251.
  16. Jing, X. et al. (2010) J. Cancer Res. Clin. Oncol. 16:1671.
Entrez Gene IDs
350 (Human); 11818 (Mouse); 287774 (Rat)
Alternate Names
Activated protein C-binding protein; Anticardiolipin cofactor; APC inhibitor; APOH; apo-H; apolipoprotein H (beta-2-glycoprotein I); Apolipoprotein H; B2G1; B2GP1; B2GPI; Beta; beta(2)GPI; beta-2-glycoprotein 1; Beta-2-glycoprotein I; BG

Citation for Recombinant Human Apolipoprotein H Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Streptococcal M1 protein constructs a pathological host fibrinogen network.
    Authors: Macheboeuf P, Buffalo C, Fu CY, Zinkernagel AS, Cole JN, Johnson JE, Nizet V, Ghosh P
    Nature, 2011-04-07;472(7341):64-8.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot

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