Recombinant Human ASAM Protein, CF

Catalog #: 5019-AM Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
5019-AM-050

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human ASAM Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. When 4 x 10⁴ cells/well are added to Recombinant Human ASAM coated plates (30 µg/mL, 100 µL/well), approximately 25%‑50% will adhere after one hour at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.

Source

Chinese Hamster Ovary cell line, CHO-derived human ASAM protein
Gly18-Met233, with a C-terminal 6-His tag

Accession #

Q9H6B4

N-terminal Sequence
Analysis

Gly18

Predicted Molecular Mass

25.2 kDa

SDS-PAGE

33-37 kDa, reducing conditions

Product Datasheets

5019-AM

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5019-AM

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: ASAM

Adipocyte-specific adhesion molecule (ASAM), also known as ACAM and coxsackie- and adenovirus receptor-like membrane protein (CLMP), is a type I transmembrane protein and member of the CTX family within the immunoglobulin superfamily. Mature human ASAM consists of a 217 amino acid (aa) extracellular region, a 21 aa transmembrane region, and a 117 aa cytoplasmic tail. The extracellular region contains one V-type and one C-type immunoglobulin domain connected by a J segment (1, 2). Within the ECD, human ASAM shares 97% aa sequence identity with mouse and rat ASAM. ASAM is widely expressed as 44 kDa and 48 kDa species in epithelial tissues where it localizes to tight junctions (1, 3). It mediates cell‑cell adhesion and contributes to trans-epithelial resistance (1). It is down‑regulated in Sertoli cells by TNF-alpha which also inhibits the expression of several other tight junction proteins in seminiferous tubules (4, 5). ASAM is additionally expressed by mature adipocytes and is up‑regulated in white adipose tissue under conditions of obesity (2).

References

Raschperger et al. (2004) J. Biol. Chem. 279:796.
Eguchi, J. et al. (2005) Biochem. J. 387:343.
Sze, K.-L. et al. (2008) J. Cell Physiol 214:334.
Sze, K.-L. et al. (2008) Biochem. J. 410:575.
Li, M.W. et al. (2006) J. Endocrinol. 190:313.

Long Name

Adipocyte-specific Adhesion Molecule

Entrez Gene IDs

79827 (Human); 71566 (Mouse); 286939 (Rat)

Alternate Names

ACAM; ACAMCoxsackie- and adenovirus receptor-like membrane protein; Adipocyte adhesion molecule; adipocyte-specific adhesion molecule; ASAM; ASAMCAR-like membrane protein; Asp5; CLMP; CXADR-like membrane protein; FLJ22415