What is the half-life of this BMP protein?

Name: Recombinant Human BMP-15 Protein
Brand: R&D Systems
SKU: 5096-BM
Rating: 4 (2 reviews)

R&D Systems has not determined the half-life of this recombinant human BMP protein. Assay conditions for each protein will need to be optimized by each researcher.

Recombinant Human BMP-15 Protein

Catalog #: 5096-BM
19 Citations 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
5096-BM-005

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Product Details

Citations (19)

FAQs

Supplemental Products

Reviews (2)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human BMP-15 Protein Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce Smad3 phosphorylation in P19 mouse embryonal carcinoma cells. Approximately 10 ng/mL can effectively induce Smad3 phosphorylation.

Source

Chinese Hamster Ovary cell line, CHO-derived human BMP-15/GDF-9B protein
Gln268-Arg392 (Thr266Arg)

Accession #

O95972

N-terminal Sequence
Analysis

Gln268

Predicted Molecular Mass

13.9 kDa

SDS-PAGE

15 kDa, reducing conditions

Product Datasheets

5096-BM

Product Datasheet

COA

SDS

5096-BM

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: BMP-15/GDF-9B

Bone morphogenetic protein 15 (BMP-15), also known as GDF-9B, is a TGF-beta superfamily ligand that is expressed by oocytes throughout folliculogenesis, and plays an important role in oocyte development (1). BMP-15 promotes the FSH‑independent proliferation of ovarian granulosa cells (GC) and induces GC glycolysis and cholesterol synthesis (2‑4). It also induces GC production of stem cell factor which, in turn, negatively regulates BMP-15 expression in oocytes (5). BMP-15 blocks the FSH‑induced GC expression of FSH R and multiple steroidogenic molecules (6). BMP-15 is synthesized with a 249 amino acid (aa) N-terminal propeptide (7). The propeptide is cleaved intracellularly from the 50 kDa proBMP-15 but remains associated with mature BMP-15 (8). Mature BMP-15 exists in 16 kDa and 17 kDa forms which are distinguishable by the presence of O-linked glycosylation on the 17 kDa form (8). Mature BMP-15 is phosphorylated, a modification which is required for the stimulation of GC proliferation (9). BMP-15 exerts its effects through interactions with BMPR‑IB/ALK6 and BMPR‑II (9-11). Mature BMP-15 forms 34 kDa noncovalently-linked homodimers and 37 kDa heterodimers with mature GDF-9 (12). Both of these oocyte-expressed factors lack the cysteine that mediates disulfide-linked dimerization in most TGF-beta superfamily ligands (1). Although heterodimerization with GDF-9 may limit the secretion of active BMP‑15, these two factors synergize in promoting oocyte survival and folliculogenesis (12, 13). Mature human BMP-15 shares 70%, 68%, and 78% aa sequence identity with mouse, rat, and sheep BMP-15, respectively. It shares 27%-38% aa sequence with other BMPs.

References

Moore, R.K. and S. Shimasaki (2005) Mol. Cell. Endocrinol. 234:67.
Otsuka, F. et al. (2000) J. Biol. Chem. 275:39523.
Sugiura, K. et al. (2007) Development 134:2593.
Su, Y.-Q. et al. (2008) Development 135:111.
Otsuka, F. and S. Shimasaki (2002) Proc. Natl. Acad. Sci. 99:8060.
Otsuka, F. et al. (2001) J. Biol. Chem. 276:11387.
Dube, J.L. et al. (1998) Mol. Endocrinol. 12:1809.
Saito, S. et al. (2008) Prot. Sci. 17:362.
McMahon, H.E. et al. (2008) Endocrinology 149:812.
Moore, R.K. et al. (2003) J. Biol. Chem. 278:304.
Edwards, S.J. et al. (2008) Endocrinology 149:1026.
Liao, W.X. et al. (2003) J. Biol. Chem. 278:3713.
Yan, C. et al. (2001) Mol. Endocrinol. 15:854.

Long Name

Bone Morphogenetic Protein 15

Entrez Gene IDs

9210 (Human); 12155 (Mouse); 59302 (Rat)

Alternate Names

BMP15; BMP-15; bone morphogenetic protein 15; GDF-9B; Growth/differentiation factor 9B; ODG2; POF4

Related Research Areas

Citations for Recombinant Human BMP-15 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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Generation of functional oocytes from male mice in vitro
Authors: K Murakami, N Hamazaki, N Hamada, G Nagamatsu, I Okamoto, H Ohta, Y Nosaka, Y Ishikura, TS Kitajima, Y Semba, Y Kunisaki, F Arai, K Akashi, M Saitou, K Kato, K Hayashi
Nature, 2023-03-15;615(7954):900-906.
Species: Mouse
Sample Types: Transfected Whole Cells
Applications: Bioassay
Cooperative effects of oocytes and estrogen on the forkhead box L2 expression in mural granulosa cells in mice
Authors: H Ito, C Emori, M Kobayashi, N Maruyama, W Fujii, K Naito, K Sugiura
Scientific Reports, 2022-11-23;12(1):20158.
Species: Mouse
Sample Types:
Applications: Bioassay
Mutual Effects of Orexin and Bone Morphogenetic Proteins on Gonadotropin Expression by Mouse Gonadotrope Cells
Authors: Y Soejima, N Iwata, N Nakayama, S Hirata, Y Nakano, K Yamamoto, A Suyama, K Oguni, T Nada, S Fujisawa, F Otsuka
International Journal of Molecular Sciences, 2022-08-29;23(17):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Bone morphogenetic protein 15 intrafollicular injection inhibits ovulation in cattle
Authors: CS Haas, FC Oliveira, MT Rovani, JG Ferst, SF Vargas, AD Vieira, RG Mondadori, LMC Pegoraro, PBD Gonçalves, V Bordignon, R Ferreira, BG Gasperin
Theriogenology, 2022-02-10;182(0):148-154.
Species: Bovine
Sample Types: In Vivo
Applications: In Vivo
Activation-induced cytidine deaminase is a possible regulator of cross-talk between oocytes and granulosa cells through GDF-9 and SCF feedback system
Authors: T Iizuka, K Wakae, M Ono, T Suzuki, Y Mizumoto, K Kitamura, SI Horike, M Muramatsu, H Fujiwara
Scientific Reports, 2021-02-15;11(1):3833.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Cyclooxygenase 2 messenger RNA levels in canine follicular cells: interrelationship with GDF-9, BMP-15, and progesterone
Authors: M De Los Rey, J Palomino, A Araujo, J Flores, G Ramirez, VH Parraguez, K Aspee
Domest. Anim. Endocrinol., 2020-08-06;74(0):106529.
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay
Cellular localization and seasonal variation in BMP15 expression in ovary of the catfish Clarias batrachus and its role in ovarian steroidogenesis
Authors: H Yadav, B Lal
Theriogenology, 2019-02-14;129(0):14-22.
Species: Fish - Clarias batrachus (Freshwater Catfish)
Sample Types: Tissue Explants
Applications: Bioassay
Effects of MiR-375-BMPR2 as a Key Factor Downstream of BMP15/GDF9 on the Smad1/5/8 and Smad2/3 Signaling Pathways
Authors: C Liu, B Yuan, H Chen, M Xu, X Sun, J Xu, Y Gao, C Chen, H Jiang, J Zhang
Cell. Physiol. Biochem., 2018-03-21;46(1):213-225.
Species: Bovine
Sample Types: Whole Cells
Applications: Bioassay
In vitro differentiation of human embryonic stem cells into ovarian follicle-like cells
Authors: D Jung, J Xiong, M Ye, X Qin, L Li, S Cheng, M Luo, J Peng, J Dong, F Tang, W Shen, MM Matzuk, K Kee
Nat Commun, 2017-06-12;8(0):15680.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Conserved miR-10 family represses proliferation and induces apoptosis in ovarian granulosa cells
Authors: T Jiajie, Y Yanzhou, AC Hoi-Hung, C Zi-Jiang, C Wai-Yee
Sci Rep, 2017-01-23;7(0):41304.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Natriuretic peptide type C induces sperm attraction for fertilization in mouse
Authors: N Kong, X Xu, Y Zhang, Y Wang, X Hao, Y Zhao, J Qiao, G Xia, M Zhang
Sci Rep, 2017-01-05;7(0):39711.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Cumulin, an Oocyte-secreted Heterodimer of the Transforming Growth Factor-beta Family, Is a Potent Activator of Granulosa Cells and Improves Oocyte Quality.
Authors: Mottershead D, Sugimura S, Al-Musawi S, Li J, Richani D, White M, Martin G, Trotta A, Ritter L, Shi J, Mueller T, Harrison C, Gilchrist R
J Biol Chem, 2015-08-08;290(39):24007-20.
Species: Human
Sample Types: Protein
Applications: Western Blot

Bone morphogenetic protein 15 in the pro-mature complex form enhances bovine oocyte developmental competence.
Authors: Sudiman J, Sutton-McDowall M, Ritter L, White M, Mottershead D, Thompson J, Gilchrist R
PLoS ONE, 2014-07-24;9(7):e103563.
Species: Human
Sample Types: Protein, Whole Cells
Applications: Bioassay, Western Blot

BMP15 suppresses progesterone production by down-regulating StAR via ALK3 in human granulosa cells.
Authors: Chang H, Cheng J, Klausen C, Leung P
Mol Endocrinol, 2013-10-18;27(12):2093-104.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Genome-wide association studies identify two novel BMP15 mutations responsible for an atypical hyperprolificacy phenotype in sheep.
Authors: Demars J, Fabre S, Sarry J, Rossetti R, Gilbert H, Persani L, Tosser-Klopp G, Mulsant P, Nowak Z, Drobik W, Martyniuk E, Bodin L
PLoS Genet, 2013-04-25;9(4):e1003482.
Species: Human
Sample Types: Whole Cells
Applications: Control

Signalling pathways mediating specific synergistic interactions between GDF9 and BMP15.
Authors: Mottershead D, Ritter L, Gilchrist R
Mol Hum Reprod, 2011-09-12;18(3):121-8.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth.
Authors: Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV
J. Biol. Chem., 2011-07-07;286(34):30034-46.
Species: Human, Mouse
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance

Steady-state level of bone morphogenetic protein-15 in goat ovaries and its influence on in vitro development and survival of preantral follicles.
Authors: Celestino JJ, Lima-Verde IB, Bruno JB, Matos MH, Chaves RN, Saraiva MV, Silva CM, Faustino LR, Rossetto R, Lopes CA, Donato MA, Peixoto CA, Campello CC, Silva JR, Figueiredo JR
Mol. Cell. Endocrinol., 2011-02-17;338(1):1-9.
Species: Goat
Sample Types: Whole Cells
Applications: Bioassay

Fibroblast growth factors and epidermal growth factor cooperate with oocyte-derived members of the TGFbeta superfamily to regulate Spry2 mRNA levels in mouse cumulus cells.
Authors: Sugiura K, Su YQ, Li Q, Wigglesworth K, Matzuk MM, Eppig JJ
Biol. Reprod., 2009-06-24;81(5):833-41.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

FAQs

What is the half-life of this BMP protein?
- R&D Systems has not determined the half-life of this recombinant human BMP protein. Assay conditions for each protein will need to be optimized by each researcher.

View all Proteins and Enzyme FAQs