Home / BMP-2/BMP-7 Heterodimer / Recombinant Human BMP-2/BMP-7 Heterodimer Protein

Recombinant Human BMP-2/BMP-7 Heterodimer Protein

Catalog #: 3229-BM
17 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
3229-BM-010/CF

With Carrier

Catalog # Availability Size / Price Qty
3229-BM-010
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Product Details
Citations (17)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human BMP-2/BMP-7 Heterodimer Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 15.0-120 ng/mL.
Source
E. coli-derived human BMP-2/BMP-7 Heterodimer protein
Human BMP-2
(Ala284 - Arg396)
Accession # NP_001191
Human BMP-7
(Ser293 - His431)
Accession # NP_001710
N-terminus C-terminus
Accession #
NP_001191 (BMP-2) & NP_001710 (BMP-7)
N-terminal Sequence
Analysis
Ala284 & Ser293
Structure / Form
Disulfide-linked heterodimer
Predicted Molecular Mass
12.9 kDa (BMP-2), 15.8 kDa (BMP-7)

Product Datasheets

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3229-BM (with carrier)

Product Datasheet
COA
SDS

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3229-BM/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3229-BM

Formulation Supplied as 0.2 μm filtered solution in 30%(v/v) Acetonitrile and 0.1%(v/v) TFA with 50 μg BSA per 1 μg as a carrier protein.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

3229-BM/CF

Formulation Supplied as a 0.2 μm filtered solution in Acetonitrile and TFA.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-2/BMP-7 Heterodimer

Human BMP-7, also known as osteogenic protein 1 (OP-1), and BMP-2 are members of the BMP subgroup of the TGF-beta superfamily and signal through heterodimeric complexes composed of type I and type II BMP receptors. BMP-2 and BMP-7 influence a variety of morphogenic processes, particularly during skeletal and renal development (1 - 3). The human BMP-2 cDNA encodes a 396 amino acid (aa) precursor that contains a 23 aa signal sequence, a 259 propeptide, and a 114 aa mature protein (4). The human BMP-7 cDNA encodes a 431 aa precursor that contains a 29 aa signal sequence, a 263 aa propeptide, and a 139 aa mature protein (5). BMP propeptides are removed by proteolysis, enabling mature BMPs to form active disulfide linked homodimers or heterodimers (1). Human and mouse BMP-2 and BMP-7 are 100% and 98% identical, respectively, at the amino acid level. Human BMP-2 shares 85% aa sequence identity with human BMP-4 and less than 51% aa sequence identity with other BMPs. Human BMP-7 shares approximately 60% - 70% aa sequence identity with BMP-5, -6, and -8, and less than 50% aa sequence identity with other BMPs. BMP-2 and BMP-7 are co-expressed in some embryonic tissues (6, 7) and associate into a functional 38 kDa osteogenic dimer (8). In in vitro osteoblast differentiation assays and in vivo bone formation models, a BMP-2/BMP-7 heterodimer is significantly more potent than either homodimer (9 - 12). Considering that BMP-2 preferentially binds BMPRIA/ALK-3 and BMPRIB/ALK-6, while BMP-7 is selective for ALK-2, the observed increase in heterodimer activity may be due to the triggering of additional receptor subtypes.

References
  1. Chen, D. et al. (2004) Growth Factors 22:233.
  2. Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251.
  3. Simic, P. and S. Vukicevic (2005) Cytokine Growth Factor Rev. 16:299.
  4. Wozney, J. et al. (1988) Science 242:1528.
  5. Gregory, K.E. et al. (2005) J. Biol. Chem. 280:27970.
  6. Lyons, K.M. et al. (1995) Mech. Dev. 50:71.
  7. Knosp, W.M. et al. (2004) Development 131:4581.
  8. Sampath, T.K. et al. (1990) J. Biol. Chem. 265:13198.
  9. Hazama, M. et al. (1995) Biochem. Biophys. Res. Commun. 209:859.
  10. Zhu, W. et al. (2004) J. Bone Miner. Res. 19:2021.
  11. Zhao, M. et al. (2005) J. Cell. Biochem. 95:1.
  12. Israel, D.I. et al. (1996) Growth Fact ors 13:291.
Long Name
Bone Morphogenetic Protein 2/7
Entrez Gene IDs
650 (Human)
Alternate Names
BDA2;BMP2A;Bone morphogenetic protein 2;SSFSC;SSFSC1; BMP-2/BMP-7 Heterodimer

Citations for Recombinant Human BMP-2/BMP-7 Heterodimer Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Suppression of heterotopic ossification in fibrodysplasia ossificans progressiva using AAV gene delivery
    Authors: YS Yang, JM Kim, J Xie, S Chaugule, C Lin, H Ma, E Hsiao, J Hong, H Chun, EM Shore, FS Kaplan, G Gao, JH Shim
    Oncogene, 2022-10-19;13(1):6175.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. The BMP signaling gradient is interpreted through concentration thresholds in dorsal-ventral axial patterning
    Authors: H Greenfeld, J Lin, MC Mullins
    PloS Biology, 2021-01-22;19(1):e3001059.
    Species: Zebrafish
    Sample Types: Embryo
    Applications: Bioassay
  3. A RUNX2 stabilization pathway mediates physiologic and pathologic bone formation
    Authors: JM Kim, YS Yang, KH Park, X Ge, R Xu, N Li, M Song, H Chun, S Bok, JF Charles, O Filhol-Coc, B Boldyreff, T Dinter, PB Yu, N Kon, W Gu, T Takarada, MB Greenblatt, JH Shim
    Nat Commun, 2020-05-08;11(1):2289.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. Bone Morphogenetic Proteins for Nucleus Pulposus Regeneration
    Authors: A Krouwels, JD Iljas, AHM Kragten, WJA Dhert, FC Öner, MA Tryfonidou, LB Creemers
    Int J Mol Sci, 2020-04-14;21(8):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  5. Feather arrays are patterned by interacting signalling and cell density waves
    Authors: WKW Ho, L Freem, D Zhao, KJ Painter, TE Woolley, EA Gaffney, MJ McGrew, A Tzika, MC Milinkovit, P Schneider, A Drusko, F Matthäus, JD Glover, KL Wells, JA Johansson, MG Davey, HM Sang, M Clinton, DJ Headon
    PLoS Biol., 2019-02-21;17(2):e3000132.
    Species: Chicken
    Sample Types: Tissue Explants
    Applications: Bioassay
  6. BLNCR is a long non-coding RNA adjacent to integrin beta-1 that is rapidly lost during epidermal progenitor cell differentiation
    Authors: SEJ Tanis, ES Köksal, JAGL van Buggen, KW Mulder
    Sci Rep, 2019-01-10;9(1):31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. HACE1 is a potential tumor suppressor in osteosarcoma
    Authors: AM El-Naggar, PW Clarkson, GL Negri, B Turgu, F Zhang, MS Anglesio, PH Sorensen
    Cell Death Dis, 2019-01-08;10(1):21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Splicing and Chromatin Factors Jointly Regulate Epidermal Differentiation
    Authors: SEJ Tanis, PWTC Jansen, H Zhou, SJ van Heerin, M Vermeulen, M Kretz, KW Mulder
    Cell Rep, 2018-10-30;25(5):1292-1303.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. All-trans retinoic-acid inhibits heterodimeric bone morphogenetic protein 2/7-stimulated osteoclastogenesis, and resorption activity
    Authors: W Bi, Y Liu, J Guo, Z Lin, J Liu, M Zhou, D Wismeijer, JL Pathak, G Wu
    Cell Biosci, 2018-08-23;8(0):48.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Bone formation by heterodimers through non-viral gene delivery of BMP-2/6 and BMP-2/7
    Authors: LD Loozen, A Vanderstee, AH Kragten, FC Öner, WJ Dhert, MC Kruyt, J Alblas
    Eur Cell Mater, 2018-03-28;35(0):195-208.
    Applications: ELISA (Standard)
  11. Directing Differentiation of Pluripotent Stem Cells Toward Retinal Pigment Epithelium Lineage
    Authors: P Choudhary, H Booth, A Gutteridge, B Surmacz, I Louca, J Steer, J Kerby, PJ Whiting
    Stem Cells Transl Med, 2016-08-24;6(2):490-501.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. p38alpha MAPK is required for tooth morphogenesis and enamel secretion.
    Authors: Greenblatt M, Kim J, Oh H, Park K, Choo M, Sano Y, Tye C, Skobe Z, Davis R, Park J, Bei M, Glimcher L, Shim J
    J Biol Chem, 2014-11-18;290(1):284-95.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  13. A novel strategy for enrichment and isolation of osteoprogenitor cells from induced pluripotent stem cells based on surface marker combination.
    Authors: Ochiai-Shino H, Kato H, Sawada T, Onodera S, Saito A, Takato T, Shibahara T, Muramatsu T, Azuma T
    PLoS ONE, 2014-06-09;9(6):e99534.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. FRMD4A upregulation in human squamous cell carcinoma promotes tumor growth and metastasis and is associated with poor prognosis.
    Authors: Goldie S, Mulder K, Tan D, Lyons S, Sims A, Watt F
    Cancer Res, 2012-05-07;72(13):3424-36.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth.
    Authors: Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV
    J. Biol. Chem., 2011-07-07;286(34):30034-46.
    Species: Human, Mouse
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  16. Bone morphogenetic protein heterodimers assemble heteromeric type I receptor complexes to pattern the dorsoventral axis.
    Authors: Little SC, Mullins MC
    Nat. Cell Biol., 2009-04-19;11(5):637-43.
    Species: Zebrafish
    Sample Types: Whole Tissue
    Applications: Bioassay
  17. Tailbud-derived Bmp4 drives proliferation and inhibits maturation of zebrafish chordamesoderm.
    Authors: Esterberg R, Delalande JM, Fritz A
    Development, 2008-10-23;135(23):3891-901.
    Species: Zebrafish
    Sample Types: In Vivo
    Applications: In Vivo

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