Recombinant Human C1qTNF10/C1qL2 Protein, CF

Catalog #: 9189-TN Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9189-TN-050

Recombinant Human C1qTNF10/C1qL2 Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human C1qTNF10/C1qL2 Protein, CF Summary

Product Specifications

Purity

>70%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human C1qTNF10/C1qL2 is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Human BAI2 Protein Fc Chimera (Catalog # 9338-BA) that produces 50% of the optimal binding response is approximately 0.075-0.45 μg/mL.

Source

E. coli-derived human C1qTNF10/C1qL2 protein
Phe154-Asp287, with an N-terminal Met and 6-His tag

Accession #

Q7Z5L3

N-terminal Sequence
Analysis

Met

Structure / Form

Noncovalently-linked homotrimer

Predicted Molecular Mass

16 kDa

SDS-PAGE

14 kDa, reducing conditions

Product Datasheets

9189-TN

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9189-TN

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, TCEP and Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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When Recombinant Human C1qTNF10 (Catalog # 9189-TN) is coated at 2 μg/mL, 100 μL/well, Recombinant Human BAI2 Fc Chimera binds with a ED₅₀ of 0.075-0.45 μg/mL.

Reconstitution Calculator

Background: C1qTNF10/C1qL2

C1qTNF10 (CTRP10), also known as C1qL2, is an approximately 30 kDa member of the C1q family of secreted proteins (1, 2). Mature human C1qTNF10 contains a collagen-like region and one C1q-like domain. It can form heterotrimers and higher order disulfide linked oligomers with C1qTNF1, C1qTNF11/C1qL4, and C1qTNF13/C1qL3 (3-6). Within the C1q-like domain, human C1qTNF10 shares 100% aa sequence identity with mouse and rat C1qTNF10. Similarly to C1qTNF11, C1qTNF13, and C1qTNF14/C1qL1, C1qTNF10 binds to BAI3 in the cerebral cortex and on cerebellar Purkinje cells (7). It is produced by mossy fibers in the hippocampus where it enhances the postsynaptic clustering of kainate-type glutamate receptors (KAR) on CA3 pyramidal neurons (8). This function is enhanced by the binding of C1qTNF10 to the KAR subunits GluK2 and GluK4 as well as to Neurexin-3 beta containing the exon 5b splice site insertion (8).

References

Grebrehiwet, B. et al. (2012) Front. Immunol. 5:3.
Yuzaki, M. (2001) Eur. J. Neurosci. 32:191.
Peterson, J.M. et al. (2009) Biochem. Biophys. Res. Commun. 388:360.
Wei, Z. et al. (2011) J. Biol. Chem. 286:15652.
Wei, Z. et al. (2013) J. Biol. Chem. 288:10214.
Wong, G.W. et al. (2008) Biochem. J. 416:161.
Bolliger, M.F. et al. (2011) Proc. Natl. Acad. Sci. USA 108:2534.
Matsuda, K. et al. (2016) Neuron 90:752.

Long Name

C1q And Tumor Necrosis Factor Related Protein 10

Entrez Gene IDs

165257 (Human); 226359 (Mouse)

Alternate Names

C1q and tumor necrosis factor related protein 10; C1q-domain containing protein; C1qL2; C1qTNF10; complement C1q-like protein 2; complement component 1, q subcomponent-like 2; CTRP10