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Recombinant Human CCL4L1/MIP-1 beta Isoform LAG-1 Protein

Catalog #: 3046-MB
1 Citation Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
3046-MB-025/CF

With Carrier

Catalog # Availability Size / Price Qty
3046-MB-025
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Citations (1)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CCL4L1/MIP-1 beta Isoform LAG-1 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR5. The ED50 for this effect is 0.5-2.5 ng/mL.
Source
E. coli-derived human CCL4L1/LAG-1 protein
Ala24-Asn92
Accession #
NP_001001435
N-terminal Sequence
Analysis
Ala24
Predicted Molecular Mass
7.8 kDa
SDS-PAGE
12 kDa, reducing conditions

Product Datasheets

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3046-MB (with carrier)

Product Datasheet
COA
SDS

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3046-MB/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3046-MB

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

3046-MB/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL4L1/LAG-1

CCL4, also known as MIP-1 beta, is a 12 kDa beta chemokine that is secreted by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells. CCL4 interacts with CCR5 to attract lymphocytes, NK cells, and immature dendritic cells to sites of inflammation (1 - 4). CCL4 also blocks the entry of HIV into CCR5-expressing cells (5). Human CCL4 and its variants are encoded by two paralogous genes(6 - 8), one of which (SCYA4) encodes CCL4 itself (9). The other gene (SCYA4L) is found in two alleles that give rise to CCL4L1 and CCL4L2, respectively (9). The human CCL4L1 cDNA encodes a 92 amino acid (aa) precursor with a 23 aa signal sequence. Alternate splicing yields an isoform with a 40 aa internal deletion. The second allele (SCYA4L2), encoding CCL4L2, is spliced into a variety of isoforms (9). Human CC4L1 shares greater than 98% aa sequence identity with CCL4 and CCL4L2. It shares 96% aa sequence identity with rhesus CCL4 and approximately 80 - 90% aa sequence identity with bovine, mouse, rabbit, and rat CCL4. The gene copy number for CCL4L1 varies from zero to five, but this is not associated with changes in mRNA or protein levels (10). CCL4 and CCL4L1 demonstrate comparable CCR5 binding, promotion of chemotaxis, and inhibition of HIV replication in stimulated PBMC (10, 11). In vivo, the first two N-terminal amino acids of CCL4 are removed by DPPIV. The truncated form is as active as full-length CCL4 and gains the ability to bind CCR1 and CCR2b (12, 13). Similar proteolytic processing of CCL4L1 has not been described.

References
  1. Maurer, M. and E. von Stebut (2004) Int. J. Biochem. Cell Biol. 36:1882.
  2. Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.
  3. Bisset, L.R. and P. Schmid-Grendelmeier (2005) Curr. Opin. Pulm. Med. 11:35.
  4. Frangogiannis, N.G. (2004) Inflamm. Res. 53:585.
  5. Lehner, T. (2002) Trends Immunol. 23:347.
  6. Modi, W.S. (2004) Genomics 83:735.
  7. Lu, J. et al. (2004) Immunogenetics 55:706.
  8. Modi, W.S. (2001) Immunogenetics 53:543.
  9. Colobran, R. et al. (2005) J. Immunol. 174:5655.
  10. Townson, J.R. et al. (2002) Eur. J. Immunol. 32:3016.
  11. Howard, O.M.Z. et al. (2004) Biochem. Biophys. Res. Commun. 320:927.
  12. Guan, E. et al. (2004) J. Cell. Biochem. 92:53.
  13. Guan, E. et al. (2002) J. Biol. Chem. 277:32348.
Entrez Gene IDs
9560 (Human)
Alternate Names
AT744.2; C-C motif chemokine 4-like; CCL4L1; CCL4Lsmall inducible cytokine A4-like; chemokine (C-C motif) ligand 4-like 1; LAG1; LAG-1; LAG1CCL4L2; LAG-1chemokine (C-C motif) ligand 4-like; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1-beta; macrophage inflammatory protein-1b2; MIP-1-beta; Monocyte adherence-induced protein 5-alpha; SCYA4L; SCYA4L1; SCYA4L2; Small-inducible cytokine A4-like

Citation for Recombinant Human CCL4L1/MIP-1 beta Isoform LAG-1 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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