Recombinant Human CD177 Fc Chimera Protein, CF

Catalog #: 11238-CD Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11238-CD-050

Recombinant Human CD177 Fc Chimera Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD177 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD177 Fc Chimera (Catalog # 11238-CD) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human Podoplanin Fc Chimera (Catalog # 3670-PL) binds with an ED₅₀ of 0.600-7.20 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human CD177 protein

Human CD177 (Leu22-Gly407) Accession # AAH29167.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

AAH29167.1

N-terminal Sequence
Analysis

Leu22

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

68 kDa

SDS-PAGE

83-93 kDa, under reducing conditions.

Product Datasheets

11238-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11238-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human CD177 Fc Chimera Protein (Catalog # 11238-CD) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human Podoplanin Fc Chimera Protein (3670-PL) binds with an ED₅₀ of 0.600-7.20 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human CD177 Fc Chimera Protein (Catalog # 11238-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 83-93 kDa and 160-180 kDa, respectively.

Reconstitution Calculator

Background: CD177

CD177, also known as NB1, human neutrophil antigen-2a (HNA-2a), and polycythemia rubra vera-1 (PRV-1), is an approximately 50-60 kDa glycoprotein member of the uPAR/CD59/Ly6 superfamily (1). Mature human CD177 is a 387 amino acid (aa) protein that contains two uPAR/Ly6 domains (2, 3). It lacks the first two uPAR domains that are present in mouse and rat CD177. Mature human CD177 shares 55% aa sequence identity with common regions of mouse and rat CD177. Alternative splicing of human CD177 generates additional isoforms that are truncated either just before or just after the first uPAR domain. CD177 is expressed on the surface of neutrophils through a glycosylphosphatidylinositol (GPI) anchor (2, 4, 5). It is nearly absent from neutrophils from paroxysmal nocturnal hemoglobinurea patients who are deficient in the ability to synthesize GPI linkages (5, 7). It is up-regulated on granulocytes from polycythemia vera and thalassemia patients (3, 6). CD177 binds to PECAM-1 on vascular endothelial cells, an interaction which mediates neutrophil adhesion to the vascular wall and neutrophil transmigration (8). It associates in cis with the Integrin MAC-1 (CD11b/CD18) (9). CD177 also associates in cis with Proteinase 3 (PR3) and is required for cell surface PR3 expression (9-11). PR3 is normally found in intracellular vesicles, but once at the cell surface it can serve as an autoimmune target for anti-neutrophil cytoplasmic antibodies (ANCA) (12). The ANCA targeting of CD177-PR3 complexes triggers neutrophil activation and vasculitis (9, 12).

References

Stroncek, D.F. (2007) Curr. Opin. Hematol. 14:688.
Kissel, K. et al. (2001) Eur. J. Immunol. 31:1301.
Temerinac, S. et al. (2000) Blood 95:2569.
Skubitz, K.M. et al. (1991) J. Leukoc. Biol. 49:163.
Klippel, S. et al. (2002) Blood 100:2441.
Zoi, K. et al. (2008) Brit. J. Haematol. 141:100.
Boccuni, P. et al. (2000) Crit. Rev. Oncol. Hematol. 33:25.
Sachs, U.J.H. et al. (2007) J. Biol. Chem. 282:23603.
Jerke, U. et al. (2011) J. Biol. Chem. 286:7070.
von Vietinghoff, S. et al. (2007) Blood 109:4487.
Kuckleburg, C.J. et al. (2012) J. Immunol. 188:2419.
van Timmeren, M.M. and P. Heeringa (2012) Curr. Opin. Rheumatol. 24:8.

Long Name

Polycythemia Rubra Vera Protein 1

Entrez Gene IDs

57126 (Human); 68891 (Mouse)

Alternate Names

CD177 antigenNB1 GP; CD177 molecule; CD177; HNA-2a; HNA2Acell surface receptor; Human neutrophil alloantigen 2a; NB1 GP; NB1PRV-1; polycythemia rubra vera 1; Polycythemia rubra vera protein 1; PRV1; PRV1NB1 glycoprotein