Recombinant Human CD3 delta/CD3d Fc Avi-tag Protein, CF

Catalog #: AVI10280 Datasheet / COA / SDS

Biotinylated

Catalog #	Availability	Size / Price	Qty
AVI10280-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD3 delta/CD3d Fc Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Recombinant Human CD3 epsilon Fc Chimera Protein (Catalog # 9850-CD) is immobilized at 10 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human CD3 delta/CD3d Fc Chimera Avi-tag (Catalog # AVI10280) that produces 50% of the optimal binding response is approximately 2.0-14 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human CD3 delta/CD3d protein

Human CD3 delta/CD3d (Phe22-Ala105) Accession # P04234.1	IEGRMD	Human IgG₁ (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Accession #

P04234.1

N-terminal Sequence
Analysis

Phe22

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Predicted Molecular Mass

38 kDa

SDS-PAGE

54-61 kDa, under reducing conditions

Product Datasheets

AVI10280

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI10280

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human CD3 epsilon Fc Chimera Protein (9850-CD) is immobilized at 10 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human CD3 delta/CD3d Fc Chimera Avi-tag (Catalog # AVI10280) that produces 50% of the optimal binding response is approximately 2.0-14 μg/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human CD3 delta/CD3d Fc Chimera Avi-tag (Catalog # AVI10280) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 54-61 kDa and 108-122 kDa, respectively.

Reconstitution Calculator

Background: CD3 delta/CD3d

CD3d, also known as CD3 delta and T3D, is a single-pass type I membrane protein. CD3 delta/CD3d is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction (1). The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T cells (2). CD3 delta/CD3d contains an 84 amino acid extracellular domain, a 21 amino acid transmembrane domain, and a 45 amino acid cytoplasmic domain. Human CD3 delta/CD3d shares 89%, 61%, and 57% sequence identity in its extracellular domain with its cynomolgus monkey, rat, and mouse homologs, respectively. CD3 delta/CD3d, like other subunit members CD3G and CD3E of the TCR-CD3 complex, contains an extracellular Ig domain and a single immunoreceptor tyrosine-based activation motif (3, 4). Defects in CD3 delta/CD3d cause severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T-/B+/NK+ SCID) which is a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels (5, 6). Our Avi-tag Biotinylated human CD-3D Fc Chimera protein features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Gil, J. et al. (2011) J. Clin. Invest. 121:3872.
Carson, G.R. et al. (1991) J. Biol. Chem. 266:7883.
Birnbaum, M.E. et al. (2014). Proc. Natl. Acad. Sci. USA. 111:17576.
Wucherpfennig, K. et al. (2010). Cold Spring Harb Perspect. Biol. 2:a005140.
Kim, S.T. et al. (2010) J. Immunol. 185:2951.
Holst, J. et al. (2008) Nat. Immunol. 9:658.

Long Name

CD3 delta

Entrez Gene IDs

915 (Human); 12500 (Mouse); 25710 (Rat); 102133701 (Cynomolgus Monkey)

Alternate Names

CD3 antigen, delta subunit; CD3 delta; CD3d antigen; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3D; CD3-DELTA; T3DOKT3, delta chain; T-cell receptor T3 delta chain; T-cell surface glycoprotein CD3 delta chain