Recombinant Human CD43 Protein, CF

Catalog #: 9680-CD
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9680-CD-050

Recombinant Human CD43 Protein Binding Activity

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Product Details

Citations (2)

Supplemental Products

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD43 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD43 is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Human Recombinant Human Siglec‑1/CD169 (Catalog # 5197-SL) that produces 50% optimal binding response is 1-10 μg/mL.

Source

Mouse myeloma cell line, NS0-derived human CD43 protein
Ser20-Arg253, with a C-terminal 10-His tag

Accession #

P16150

N-terminal Sequence
Analysis

Ser20

Predicted Molecular Mass

25 kDa

SDS-PAGE

77-88 kDa, reducing conditions

Product Datasheets

9680-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9680-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human CD43 is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human Siglec-1/CD169 (Catalog # 5197-SL) binds with an ED₅₀ of 1-10 µg/mL.

Reconstitution Calculator

Background: CD43

CD43, also known as Leukosialin and Sialophorin and Ly-48, is a type I transmembrane sialylated mucin that is expressed on most leukocytes and some tumor cells (1). Notably, the membrane expression of CD43 seems to be a characteristic of leukocytes, while cytoplasmic expression without membrane insertion occurs in endothelium and select epithelia. While CD43 restricts leukocyte adhesion and modulates T cell activation, these activities are context specific (2). CD43 can both induce and protect against apoptosis, and can either promote or block cell adhesion (3). In human, CD43 is synthesized as a 400 amino acid (aa) precursor that contains a 19 aa signal sequence, a 234 aa extracellular region, a 23 aa TM domain, and a 124 aa cytoplasmic tail. The extracellular region (aa 20-253) has 25 O-linked glycosylation sites and 1 N-linked glycosylation site. Human CD43 extracellular region has less than 60% aa identity to the extracellular regions in rat and mouse.

References

Santamaría, M. et al. (1996). Cancer Res. 56:3526.
Manjunath, N. et al. (1995) Nature 377:535.
Brown, T. J. et al. (1996) J. Biol. Chem. 271:27686.

Long Name

Cluster of Differentiation 43

Entrez Gene IDs

6693 (Human); 20737 (Mouse); 24796 (Rat); 102147271 (Cynomolgus Monkey)

Alternate Names

CD43 antigen; CD43; CD43); Galactoglycoprotein; GALGP; GPL115; Leukocyte sialoglycoprotein; Leukosialin; LSN; Ly-48; sialophorin (gpL115, leukosialin, CD43); Sialophorin; SPN

Related Research Areas

Citations for Recombinant Human CD43 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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A previously uncharacterized O-glycopeptidase from Akkermansia muciniphila requires the Tn-antigen for cleavage of the peptide bond
Authors: BJ Medley, L Leclaire, N Thompson, KE Mahoney, B Pluvinage, MAH Parson, JE Burke, S Malaker, W Wakarchuk, AB Boraston
The Journal of Biological Chemistry, 2022-08-30;0(0):102439.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay
Structure-guided mutagenesis of a mucin-selective metalloprotease from Akkermansia muciniphila alters substrate preferences
Authors: DJ Shon, D Fernandez, NM Riley, MJ Ferracane, CR Bertozzi
The Journal of Biological Chemistry, 2022-04-09;0(0):101917.
Species: Akkermansia muciniphila
Sample Types: Protein
Applications: Bioassay