Recombinant Human CD96v2 Fc Chimera Avi-tag Protein, CF

Catalog # Availability Size / Price Qty
AVI9556-050
Biotinylated Recombinant Human CD96v2 Fc Chimera Avi-tag Protein Binding Activity.
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Recombinant Human CD96v2 Fc Chimera Avi-tag Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human CD96v2 Fc Chimera Avi-tag (Catalog # AVI9556) binds Recombinant Human CD155/PVR Fc Chimera (Catalog # 9174-CD) with an ED50 of 30.0-300 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human CD96 protein
Human CD96v2
(Lys25-Met503)
Accession # NP_005807.1
IEGRMDHuman IgG1
(Pro100-Lys330)
Avi-tag
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Lys25
Structure / Form
Disulfide-linked homodimer
Biotinylated via Avi-tag
Predicted Molecular Mass
82 kDa
SDS-PAGE
117-146 kDa, under reducing conditions.

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AVI9556

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI9556

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

Biotinylated Recombinant Human CD96v2 Fc Chimera Avi-tag Protein (Catalog # AVI9556) binds Recombinant Human CD155/PVR Fc Chimera (9174-CD) with an ED50 of 30.0-300 ng/mL.

SDS-PAGE View Larger

2 μg/lane of Biotinylated Recombinant Human CD96v2 Fc Chimera Avi-tag Protein (Catalog # AVI9556) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 117-146 kDa, and 230-290 kDa, respectively.

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Background: CD96

Human CD96, also known as Tactile (T cell-activated increased late expression), is a 160 kDa type I transmembrane glycoprotein of the Ig superfamily that shows peak expression 6-9 days after activation of T, NK, and a subpopulation of B cells (1, 2). CD96 is also frequently expressed on acute myeloid leukemia and myelodysplastic stem cells (3, 4). It is expressed on CD4+ and CD8+ T cells, plus NK cells and select B cells (5). Low expression of adhesive human CD69 is a rare cause of C syndrome, a set of developmental anomalies in cranial bone (trigonocephaly), skin and viscera, demonstrating a role for CD96 in development of these tissues (2, 6). Human CD96 has two isoforms generated by alternative splicing. Isoform 2 is the most common form of CD96 that shows a 16 amino acid (aa) deletion comparing to isoform 1. Mature human CD96 isoform 2 is a 548 amino acid (aa), type I transmembrane glycoprotein. It contains a 482 aa extracellular region that contains three Ig-like domains. The two N-terminal domains are V-type and I-type, while the distal domain is a C-type structure (2). Within the ECD, human CD96 shares 53% and 52% aa sequence identity with mouse CD96 and rat CD96, respectively (7). The ECD is highly N- and O-glycosylated (1). An 80 kDa soluble form is elevated in human serum during chronic hepatitis B (9). The most N-terminal Ig-like domain of human CD96 binds to CD155/PVR (poliovirus receptor), but CD96/CD155 interaction is species-specific (2, 7, 10). Mouse CD96 also binds Nectin-1 (7). On NK cells, costimulatory CD96 and DNAM-1 (CD226) are thought to have paired activity with inhibitory TIGIT, all of which bind CD155 and various Nectins (11, 12). CD96 can promote NK cell adhesion to, and killing of target cells, including tumors that express CD155 (10, 11). Our Avi-tag Biotinylated CD96 Fc Chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References
  1. Wang, P.L. et al. (1992) J. Immunol. 148:2600.
  2. Meyer, D. et al. (2009) J. Biol. Chem. 284:2235.
  3. Hosen, N. et al. (2007) Proc. Natl. Acad. Sci. USA 104:11008.
  4. Xie, W. et al. (2010) Cytometry A 77:840.
  5. Eriksson,E. M. et al. (2012) PLOS One 7:e51696
  6. Kaname, T. et al. (2007) Am. J. Hum. Genet. 81:835.
  7. Seth, S. et al. (2007) Biochem. Biophys. Res. Commun. 364:959.
  8. Protein Accession # BAE32358.
  9. Gong, J. et al. (2008) Clin. Exp. Immunol. 155:207.
  10. Fuchs, A. et al. (2004) J. Immunol. 172:3394.
  11. Stanietsky, N. and O. Mandelboim (2010) FEBS Lett. 584:4895.
  12. Xu, Z. and B. Jin (2010) Cell. Mol. Immunol. 7:11.
Entrez Gene IDs
10225 (Human); 84544 (Mouse); 498079 (Rat); 102121080 (Cynomolgus Monkey)
Alternate Names
CD96 antigenMGC22596; CD96 molecule; CD96; DKFZp667E2122; T cell-activated increased late expression protein; TACTILE; TACTILEincreased late expression; T-cell surface protein tactile

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