Recombinant Human CD96v2 Fc Chimera Protein, CF

Catalog #: 9556-CD Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9556-CD-050

Recombinant Human CD96v2 Fc Chimera Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD96v2 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD155/PVR Fc Chimera (Catalog # 9174-CD) is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human CD96 v2 Fc Chimera that produces 50% of the optimal binding response is 30-150 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human CD96 protein

Human CD96 (Lys25-Met503) Accession # P40200-2	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

P40200-2

N-terminal Sequence
Analysis

Lys25

Structure / Form

DIsulfide-linked homodimer

Predicted Molecular Mass

80 kDa

SDS-PAGE

132-152 kDa, reducing conditions

Product Datasheets

9556-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9556-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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When Recombinant Human CD155/PVR Fc Chimera (Catalog # 9174-CD) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human CD96v2 Fc Chimera (Catalog # 9556-CD) binds with an ED₅₀ of 30-150 ng/mL.

Reconstitution Calculator

Background: CD96

Human CD96, also known as Tactile (T cell-activated increased late expression), is a 160 kDa type I transmembrane glycoprotein of the Ig superfamily that shows peak expression 6-9 days after activation of T, NK, and a subpopulation of B cells (1, 2). CD96 is also frequently expressed on acute myeloid leukemia and myelodysplastic stem cells (3, 4). It is expressed on CD4⁺ and CD8⁺ T cells, plus NK cells and select B cells (5). Low expression of adhesive human CD69 is a rare cause of C syndrome, a set of developmental anomalies in cranial bone (trigonocephaly), skin and viscera, demonstrating a role for CD96 in development of these tissues (2, 6). Human CD96 has two isoforms generated by alternative splicing. Isoform 2 is the most common form of CD96 that shows a 16 amino acid (aa) deletion comparing to isoform 1. Mature human CD96 isoform 2 is a 548 amino acid (aa), type I transmembrane glycoprotein. It contains a 482 aa extracellular region that contains three Ig-like domains. The two N-terminal domains are V-type and I-type, while the distal domain is a C-type structure (2). Within the ECD, human CD96 shares 53% and 52% aa sequence identity with mouse CD96 and rat CD96, respectively (7). The ECD is highly N- and O-glycosylated (1). An 80 kDa soluble form is elevated in human serum during chronic hepatitis B (9). The most N-terminal Ig-like domain of human CD96 binds to CD155/PVR (poliovirus receptor), but CD96/CD155 interaction is species-specific (2, 7, 10). Mouse CD96 also binds Nectin-1 (7). On NK cells, costimulatory CD96 and DNAM-1 (CD226) are thought to have paired activity with inhibitory TIGIT, all of which bind CD155 and various Nectins (11, 12). CD96 can promote NK cell adhesion to, and killing of target cells, including tumors that express CD155 (10, 11).

References

Wang, P.L. et al. (1992) J. Immunol. 148:2600.
Meyer, D. et al. (2009) J. Biol. Chem. 284:2235.
Hosen, N. et al. (2007) Proc. Natl. Acad. Sci. USA 104:11008.
Xie, W. et al. (2010) Cytometry A 77:840.
Eriksson,E. M. et al. (2012) PLOS One 7:e51696
Kaname, T. et al. (2007) Am. J. Hum. Genet. 81:835.
Seth, S. et al. (2007) Biochem. Biophys. Res. Commun. 364:959.
Protein Accession # BAE32358.
Gong, J. et al. (2008) Clin. Exp. Immunol. 155:207.
Fuchs, A. et al. (2004) J. Immunol. 172:3394.
Stanietsky, N. and O. Mandelboim (2010) FEBS Lett. 584:4895.
Xu, Z. and B. Jin (2010) Cell. Mol. Immunol. 7:11.

Entrez Gene IDs

10225 (Human); 84544 (Mouse); 498079 (Rat); 102121080 (Cynomolgus Monkey)

Alternate Names

CD96 antigenMGC22596; CD96 molecule; CD96; DKFZp667E2122; T cell-activated increased late expression protein; TACTILE; TACTILEincreased late expression; T-cell surface protein tactile