Recombinant Human CEACAM-18 Fc Chimera Protein, CF

Catalog #: 9869-CM Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9869-CM-050

Recombinant Human CEACAM-18 Fc Chimera Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CEACAM-18 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Galectin-3 (Catalog # 8259-GA) is immobilized at 1 μg/mL, 100 μL/well, it binds Recombinant Human CEACAM-18 Fc Chimera. The concentration of Recombinant Human CEACAM-18 Fc Chimera that produces 50% of the optimal binding response is
0.5-5 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived human CEACAM-18 protein

Human CEACAM-18 (Gln31-His317) Accession # A8MTB9	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

A8MTB9

N-terminal Sequence
Analysis

No results obtained. Gln31 inferred from enzymatic pyroglutamate treatment revealing Ile32.

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

59 kDa

SDS-PAGE

93-105 kDa, reducing conditions

Product Datasheets

9869-CM

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9869-CM

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human Galectin-3 (Human Cell-expressed) (Catalog # 8259-GA) is coated at 1 µg/mL, 100 µL/well, Recombinant Human CEACAM-18 Fc Chimera (Catalog # 9869-CM) binds with an ED₅₀ of 0.5-5 µg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human CEACAM-18 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 93 -105 kDa and 190- 200 kDa, respectively.

Reconstitution Calculator

Background: CEACAM-18

Carcinoembryonic Antigen-related Cell Adhesion Molecule 18 (CEACAM-18) is part of the CEA protein family consisting of CEACAMs and the pregnancy-specific glycoproteins (PSGs). Both CEACAM and PSG molecules have been identified in humans and belong to the much larger glycosylphosphatidylinositol (GPI)-linked immunoglobulin (Ig) superfamily (1, 2). Mature human CEACAM-18 has a 298 amino acid (aa) extracellular domain containing 2 IgC2-like and 1 IgV-like domains, a single transmembrane domain and a short cytoplasmic tail (2). CEACAM-18 is one of only five conserved CEACAMs among mouse, rat, and human (2), but mature human CEACAM-18 has low aa sequence identify with mouse and rat at 60% and 58%, respectively. Originally discovered as a biomarker for colorectal cancer (3), CEACAMs have now been associated with numerous intracellular signaling processes including cell adhesion, cell growth, recognition and differentiation, angiogenesis, and apoptosis (4-6). While the exact function of CEACAM-18 has been yet to be elucidated, it may bind pathogen receptors or other immunoregulatory members (6). CEACAM family members were identified as the major Galectin-3 receptor candidates on human neutrophils (7). Binding of carbohydrate ligands to CEACAMs may be important in the release of proinflammatory mediators (8, 9).

References