Recombinant Human CEACAM-20 Fc Chimera Protein, CF

Catalog #: 9427-CM Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9427-CM-050

Recombinant Human CEACAM-20 Fc Chimera Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CEACAM-20 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line. The ED₅₀ for this effect is 0.5-2.5 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human CEACAM-20 protein

Human CEACAM-20 (Gln31-Gly450) Accession # Q6UY09	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q6UY09

N-terminal Sequence
Analysis

No results obtained. Gln31 inferred from enzymatic pyroglutamate treatment revealing Leu32

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

73 kDa

SDS-PAGE

82-105 kDa, reducing conditions

Product Datasheets

9427-CM

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9427-CM

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Immobilized Recombinant Human CEACAM-20 Fc Chimera (Catalog # 9427-CM) enhances L Cells mouse fibroblasts adhesion. The ED₅₀ for this effect is 0.5-2.5 μg/mL.

Reconstitution Calculator

Background: CEACAM-20

Carcinoembryonic antigen-related cell adhesion molecule-20 (CEACAM-20) is a member of the CEACAM subfamily of glycoproteins in the immunoglobulin (Ig) superfamily. Mature human CEACAM-20 consists of a 420 aa extracellular domain, a 21 aa helical transmembrane, and a 114 aa cytoplasmic domain. The extracellular domain possesses four IgC2-like domains, which are stabilized by disulfide bonds, as well as several predicted glycosylation sites (1-5). The extracellular domain of CEACAM-20 is also unique among the CEACAMs because it contains a truncated IgV-like N domain (2). The extracellular domain of human CEACAM-20 has 64% and 62% aa identity to the mouse and rat protein, respectively. Also, the cytoplasmic domain is unusually long compared to most other CEACAMs and is predicted to contain four tyrosine phosphorylation sites, two of which correspond to the immune-receptor tyrosine-based activation motif (ITAM) (2, 3). Human CEACAM proteins have been linked to numerous intercellular-adhesion and intracellular signaling processes including cell adhesion, growth, and recognition, differentiation, angiogenesis, and apoptosis (7, 8). Human CEACAM-20 expression is limited to the reproductive system and the intestinal tract, with the highest levels of expression found in the small intestine and prostate (2, 3). An in vitro model of human prostate morphogenesis showed that CEACAM-20 is co-expressed with CEACAM-1 and plays a critical role in the formation of prostate organoids, making it a marker for prostate cancer (2). Although the exact mechanism is not fully understood, CEACAM-20 may promote the proliferation of intestinal epithelial cells (IECs) (9). There is evidence suggesting CEACAM-20 can induce the production of chemokines like interleukin (IL)-8 and stimulate inflammatory responses in colitis and Crohn's disease (6). CEACAM-20 is also thought be act as a physiological substrate for SAP-1 in the intestinal epithelium (10).

References

Tchoupa, A. et al. (2014) J Cell Commun Signal 12:27.
Zhang, H. et al. (2013) PLoS ONE 8:e53359.
Zebhauser, R. et al. (2005) Genomics 86:566.
Beauchemin, N. Arabzadeh, A. (2013) Cancer Metastasis Rev 32(3):643.
Kuespert, K. et al. (2006) Curr Opin Cell Biol 18:565.
Murata, Y. et al. (2015) PNAS E4264-4271.
Obrink, B. (1997) Curr Opin Cell Biol 9:616.
Horst, AK. Wagener, C. (2004) Handb Exp Pharmacol 283-341.
Kitamura, Y. et al. (2015) Genes to Cells 20:578.
Kotani, et al. (2016) Expert Review of Gastroenterology & Hepatology. 10:1313.