Recombinant Human CEACAM-8/CD66b Protein, CF

Catalog #: 9639-CM Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9639-CM-050

Recombinant Human CEACAM-8/CD66b Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CEACAM-8/CD66b Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Galectin‑3 (Catalog # 1154-GA) is coated at 2 μg/mL, 100 μL/well, it binds to Recombinant CEACAM‑8/CD66b with an ED₅₀ of 0.4-2.4 μg/mL.

Source

Mouse myeloma cell line, NS0-derived human CEACAM-8/CD66b protein
Gln35-Asp320, with a C-terminal 6-His tag

Accession #

P31997

N-terminal Sequence
Analysis

Gln35

Predicted Molecular Mass

32 kDa

SDS-PAGE

67-77 kDa, reducing conditions

Product Datasheets

9639-CM

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9639-CM

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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When Recombinant Human Galectin-3 (Catalog # 1154-GA) is coated at 2 µg/mL, 100 µL/well, it binds to Recombinant CEACAM-8/CD66b (Catalog # 9639-CM) with an ED₅₀ of 0.4-2.4 µg/mL.

Reconstitution Calculator

Background: CEACAM-8/CD66b

Carcinoembryonic antigen-related cell adhesion molecule 8 (CEACAM-8), also known as CGM6 and CD66b, is an approximately 95 kDa member of the human carcinoembryonic antigen (CEA) family that plays a role in cell adhesion, cell migration and pathogen binding (1, 2). CEACAM-8 is anchored to cells by a glycophosphatidylinositol (GPI) motif and is expressed exclusively on granulocytes (3, 4). Human CEACAM-8 is composed of a 34 amino acid (aa) signal peptide, a 29 aa propeptide segment which is removed in the mature form, and a 286 aa CEACAM-8 region containing one V-type and two C2-type Ig-like domains (5). A homologue for CEACAM-8 has not been identified in the mice or rats (1). Activation enhances surface expression of CEACAM-8 and is an activation marker in human neutrophils and eosinophils (1, 6, 7). Engagement of CEACAM-8 by mAb or galectin-3 activated a Src family kinase (lyn), and induced cellular adhesion, superoxide production, and degranulation of eosinophils (1). CEACAM-8 can form both homotypic, and heterotypic bonds with CEACAM-6 through interaction of the V-type Ig-like domains (7-9). Cross-linking of neutrophil CEACAM-8 augments cytokine secretion and beta2 integrin-mediated adhesion (10, 11).

References

Yoon, J. et al. (2007) J. Immunol. 179:8454.
Feuk-Lagerstedt, E. et al. (1999) J. Immunol. 163:5592.
Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
Bonsor, D.A. et al. (2015) Proc. Natl. Acad. Sci. USA 112:13561.
Tchoupa, T. et al. (2014) Cell. Commun. Signal. 12:27.
Eades-Perner AM. et al. (1998) Blood 91:663.
Schmidt T. (2012) J Leukoc Biol. 91:791.
Kuroki, M. et al. (2001) J. Leukoc. Biol. 70:543.
Yamanka T et al. (1996) Biochem Biophys Res Commun. 219:842.
Schröder AK et al. (2006) Hum Immunol. 67:676.
Skubitz KM et al. (1996) J Leukoc Biol. 60:106.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 8

Entrez Gene IDs

1088 (Human); 102138843 (Cynomolgus Monkey)

Alternate Names

Carcinoembryonic antigen CGM6; carcinoembryonic antigen gene family member 6; carcinoembryonic antigen-related cell adhesion molecule 8; CD66b antigen; CD66b; CD67 antigen; CD67; CEACAM8; CEACAM-8; CGM6; NCA-95; Non-specific cross-reacting antigen NCA-95