Recombinant Human CLEC9a Fc Chimera Protein, CF

Catalog #: 6049-CL
3 Citations Datasheet / COA / SDS

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6049-CL-050

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Citations (3)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CLEC9a Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by flow cytometry for its ability to bind a ligand expressed in FasL-treated mouse B cells. Sancho, D. et al. (2009) Nature 458:899. At 100 ng/mL, >50% of necrotic cells bind to Recombinant Human CLEC9a.

Source

Mouse myeloma cell line, NS0-derived human CLEC9a protein

Met-Asp	Human IgG₁ (Pro100-Lys330)	IEGR	Human CLEC9a (Lys57-Val241) Accession # Q6UXN8
N-terminus			C-terminus

Accession #

Q6UXN8

N-terminal Sequence
Analysis

Met

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

47.8 kDa (monomer)

SDS-PAGE

55-65 kDa, reducing conditions

Product Datasheets

6049-CL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6049-CL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: CLEC9a

CLEC9A (C-type lectin domain family 9 member A), also known as DNGR-1, is a type II transmembrane glycoprotein member of the C-type lectin superfamily (1). Mature mouse CLEC9A consists of a 35 amino acid (aa) cytoplasmic domain with an ITAM-like motif, a 21 aa transmembrane segment, and a 185 extracellular domain (ECD) that contains a stalk region and one C-type lectin domain (CTLD) (2-4). Within the ECD, human CLEC9A shares 57% aa sequence identity with mouse and rat CLEC9A. Although the CTLD of CLEC9A structurally resembles that of other C-type lectins, it lacks the conserved residues that typically mediate calcium and carbohydrate binding. CLEC9A is expressed as a disulfide-linked homodimer of approximately 50 kDa N-glycosylated subunits (2, 4). Human CLEC9A expression is restricted to a subpopulation of BDCA-3⁺ conventional dendritic cells (cDC) and CD16^- monocytes (2-5). BDCA-3⁺ cDC are analagous to mouse CD8⁺ cDC which are specialized in antigenic cross-presentation in complex with MHC class I molecules (6). In mouse, CLEC9A is additionally expressed on plasmacytoid dendritic cells (3, 4). CLEC9A ligation enhances antigen uptake and processing, leading to presentation on MHC class I and cytotoxic T cell (CTL) priming (2-5, 7). Its recognition of filamentous actin in dead cells is important for triggering an immune response to necrotic cell debris (8-10). CLEC9A is also required for the presentation of viral proteins and the subsequent CTL-mediated killing of virus-infected cells (11, 12). CLEC9A signaling triggers activation of the tyrosine kinase Syk (2, 8).

References

Kerrigan, A.M. and G.D. Brown (2010) Immunol. Rev. 234:335.
Huysamen, C. et al. (2008) J. Biol. Chem. 283:16693.
Caminschi, I. et al. (2008) Blood 112:3264.
Sancho, D. et al. (2008) J. Clin. Invest. 118:2098.
Schreibelt, G. et al. (2012) Blood 119:2284.
Dudziak, D. et al. (2007) Science 315:107.
Poulin, L.F. et al. (2010) J. Exp. Med. 207:1261.
Sancho, D. et al. (2009) Nature 458:899.
Ahrens, S. et al. (2012) Immunity 36:635.
Zhang, J.-G. et al. (2012) Immunity 36:646.
Iborra, S. et al. (2012) J. Clin. Invest. 122:1628.
Zelenay, S. et al. (2012) J. Clin. Invest. 122:1615.

Long Name

C-type Lectin-like Receptor 9

Entrez Gene IDs

283420 (Human); 232414 (Mouse)

Alternate Names

CD370; CLEC9a; C-type lectin domain family 9 member A; C-type lectin domain family 9, member A; DNGR1; HEEE9341; UNQ9341

Related Research Areas

Citations for Recombinant Human CLEC9a Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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An innovative immunotherapeutic strategy for ovarian cancer: CLEC10A and glycomimetic peptides
Authors: LL Eggink, KF Roby, R Cote, J Kenneth Ho
J Immunother Cancer, 2018-04-17;6(1):28.
Species: Human
Sample Types: Peptide
Applications: Binding Assay
Clec4A4 is a regulatory receptor for dendritic cells that impairs inflammation and T-cell immunity
Authors: T Uto, T Fukaya, H Takagi, K Arimura, T Nakamura, N Kojima, B Malissen, K Sato
Nat Commun, 2016-04-12;7(0):11273.
Species: Human
Sample Types: Recombinant Protein
Applications: Enzyme Assay
The DC receptor DNGR-1 mediates cross-priming of CTLs during vaccinia virus infection in mice.
Authors: Iborra S, Izquierdo HM, Martinez-Lopez M, Blanco-Menendez N, Reis E Sousa C, Sancho D
J. Clin. Invest., 2012-04-16;122(5):1628-43.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry