Recombinant Human Complement Factor H-related 4/CFHR4, CF

Catalog #: 11300-CH Datasheet / COA / SDS

HEK293, His-tag

Catalog #	Availability	Size / Price	Qty
11300-CH-050

Recombinant Human Complement Factor H‑related 4/CFHR4 His-tag Protein Binding Activity.

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All Complement Factor H-related 4/CFHR4 Proteins and Enzymes

Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Complement Factor H-related 4/CFHR4, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse Complement Component C3d His-tag (Catalog # 2655-C3) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human Complement Factor H-related 4/CFHR4 His-tag (Catalog # 11300-CH) binds with an ED₅₀ of 1.50-15.0 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human Complement Factor H-related 4/CFHR4 protein
Glu20-Glu578, with a C-terminal 6-His tag

Accession #

Q92496.3

N-terminal Sequence
Analysis

Glu20

Predicted Molecular Mass

64 kDa

SDS-PAGE

92-103 kDa, under reducing conditions

Product Datasheets

11300-CH

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11300-CH

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Mouse Complement Component C3d His-tag (2655-C3) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human Complement Factor H‑related 4/CFHR4 His-tag Protein (Catalog # 11300-CH) binds with an ED₅₀ of 1.50-15.0 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human Complement Factor H-related 4/CFHR4 His-tag Protein (Catalog # 11300-CH) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 92-103 kDa.

Reconstitution Calculator

Background: Complement Factor H-related 4/CFHR4

Complement factor H-related protein 4 (CFHR4) is a secreted glycoprotein member of the factor H family of glycoproteins (1). The human complement factor H protein family consists of the complement and immune regulators factor H, the factor H-like protein 1 (FHL-1) and five factor H-related proteins (FHR-1 to -5) (1). The genes of this family are located on human chromosome 1q32, which is known as the regulator of complement activation (RCA) gene clusters (2). CFHRs are exclusively composed of individually folded protein domains, termed short consensus repeats (SCRs) or complement control modules. All CFHRs, including CFHR4, are capable of binding complement factor C3b, discriminate between self and non-self cell surfaces, and have been proposed to deregulate complement activation by inhibiting interaction of CFH with C3b and C3d (1, 3). CFHR4 activates complement through its interaction with C3b (4). CFHR4 can also bind to native (pentameric) C-reactive protein (pCRP) through its first SCR domain and is suggested to promote pCRP binding to necrotic cells surfaces, thus promoting cell clearance (5). CFHR4 is synthesized as 2 isoforms by hepatocytes and circulates in plasma. The shorter isoform, FHR4B, contains 5 of 9 SCRs. The CFHR4 product includes a duplication insertion of 247 aa and represents the longer alternative splice form termed FHR4A (6).

References