Home / CXCL16 / Recombinant Human CXCL16 Protein

Recombinant Human CXCL16 Protein

Catalog #: 976-CX
13 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
976-CX-025/CF

With Carrier

Catalog # Availability Size / Price Qty
976-CX-025
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Citations (13)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CXCL16 Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CXCR6. Matloubian, M. et al. (2000) Nat. Immunol. 1:298. The ED50 for this effect is 2.5-12 ng/mL.
Source
E. coli-derived human CXCL16 protein
Asn30 - Pro118
Accession #
Q9H2A7.4
N-terminal Sequence
Analysis
Asn30
Predicted Molecular Mass
10.2 kDa
SDS-PAGE
10 kDa, reducing conditions

Product Datasheets

You must select a language.

x

976-CX (with carrier)

Product Datasheet
COA
SDS

You must select a language.

x

976-CX/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

976-CX

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

976-CX/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: CXCL16

CXC chemokine ligand 16 (CXCL16) is a type I membrane protein containing a non-ELR motif-containing CXC chemokine domain in its extracellular region. Together with Fractalkine (CX3CL1), CXCL16 constitute the only two transmembrane chemokines within the superfamily. The gene for human CXCL16 predicts a 273 amino acid (aa) residue precursor protein with a putative signal peptide, a CXC chemokine domain, a mucin-like spacer region, a transmembrane domain and a cytoplasmic domain with a potential tyrosine phosphorylation and SH2 protein-binding site. Mouse and human CXCL16 share 70% aa sequence similarity within their chemokine domains and 49% overall aa sequence identity. By northern blot analysis, CXCL16 expression is detected in various human organs except for brain, bone marrow, skeletal muscle or colon. By flow cytometry, CXCL16 has been detected on the surface CD19+ B cells, CD14+ monocytes/macrophages, and CD11c+ splenic and lymph node dendritic cells. Functional CXCL16 can be shed from the cell surface as an approximately 35 kDa soluble protein. The functional receptor for CXCL16 has been identified as CXCR6 (also known as Bonzo, STRL33 or TYMSTR), a receptor previously shown to be a co-receptor for HIV entry. CXCL16 has also been independently cloned and named SRPSOX (scavenger receptor that binds phosphatidylserine and oxidized lipoprotein). It was shown to be a specific receptor for OxLDL but not LDL or acetyl-LDL.

References
  1. Matloubian, M. et al. (2000) Nature Immun. 1:298.
  2. Shimaoka, T. et al. (2000) J. Biol. Chem. 275:40663.
  3. Wilbanks, A. et al. (2001) J. Immun. 166:5145.
Entrez Gene IDs
58191 (Human); 66102 (Mouse)
Alternate Names
chemokine (C-X-C motif) ligand 16; CXC chemokine ligand 16; CXCL16; CXCLG16; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; SCYB16; Small-inducible cytokine B16; SRPSOXC-X-C motif chemokine 16; SR-PSOXTransmembrane chemokine CXCL16

Citations for Recombinant Human CXCL16 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Chemokines expressed by engineered bacteria recruit and orchestrate antitumor immunity
    Authors: TM Savage, RL Vincent, SS Rae, LH Huang, A Ahn, K Pu, F Li, K de Los San, C Coker, T Danino, N Arpaia
    Science Advances, 2023-03-08;9(10):eadc9436.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Bioassay
  2. Tissue CD14+CD8+ T cells reprogrammed by myeloid cells and modulated by LPS
    Authors: LJ Pallett, L Swadling, M Diniz, AA Maini, M Schwabenla, AD Gasull, J Davies, S Kucykowicz, JK Skelton, N Thomas, NM Schmidt, OE Amin, US Gill, KA Stegmann, AR Burton, E Stephenson, G Reynolds, M Whelan, J Sanchez, R de Maeyer, C Thakker, K Suveizdyte, I Uddin, AM Ortega-Pri, C Grant, F Froghi, G Fusai, S Lens, S Pérez-Del-, W Al-Akkad, G Mazza, M Noursadegh, A Akbar, PTF Kennedy, BR Davidson, M Prinz, BM Chain, M Haniffa, DW Gilroy, M Dorner, B Bengsch, A Schurich, MK Maini
    Nature, 2023-01-25;614(7947):334-342.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Organoid-on-a-chip model of human ARPKD reveals mechanosensing pathomechanisms for drug discovery
    Authors: K Hiratsuka, T Miyoshi, KT Kroll, NR Gupta, MT Valerius, T Ferrante, M Yamashita, JA Lewis, R Morizane
    Science Advances, 2022-09-21;8(38):eabq0866.
    Species: Human
    Sample Types: Organoids
    Applications: Bioassay
  4. Distinct molecular and immune hallmarks of inflammatory arthritis induced by immune checkpoint inhibitors for cancer therapy
    Authors: ST Kim, Y Chu, M Misoi, ME Suarez-Alm, JH Tayar, H Lu, M Buni, J Kramer, E Rodriguez, Z Hussain, SS Neelapu, J Wang, AY Shah, NM Tannir, MT Campbell, DL Gibbons, T Cascone, C Lu, GR Blumensche, M Altan, B Lim, V Valero, ME Loghin, J Tu, SN Westin, A Naing, G Garcia-Man, N Abdel-Waha, HA Tawbi, P Hwu, ICG Oliva, MA Davies, SP Patel, J Zou, A Futreal, A Diab, L Wang, R Nurieva
    Nature Communications, 2022-04-12;13(1):1970.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. High miR-451 expression in peripheral blood mononuclear cells from subjects at risk of developing rheumatoid arthritis
    Authors: K Prajzlerov, O Kryšt?fkov, P Hánová, V Horváthová, M Gregová, K Pavelka, J Vencovský, L Šenolt, M Filková
    Scientific Reports, 2021-02-25;11(1):4719.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. CXCR4 inhibition in human pancreatic and colorectal cancers induces an integrated immune response
    Authors: D Biasci, M Smoragiewi, CM Connell, Z Wang, Y Gao, JED Thaventhir, B Basu, L Magiera, TI Johnson, L Bax, A Gopinathan, C Isherwood, FA Gallagher, M Pawula, I Hudecova, D Gale, N Rosenfeld, P Barmpounak, EC Popa, R Brais, E Godfrey, F Mir, FM Richards, DT Fearon, T Janowitz, DI Jodrell
    Proc Natl Acad Sci U S A, 2020-10-30;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Mesenchymal stem cell-derived CXCL16 promotes progression of gastric cancer cells by STAT3-mediated expression of Ror1
    Authors: T Ikeda, M Nishita, K Hoshi, T Honda, Y Kakeji, Y Minami
    Cancer Sci., 2020-02-25;111(4):1254-1265.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Papillary renal cell carcinoma-derived chemerin, IL-8, and CXCL16 promote monocyte recruitment and differentiation into foam-cell macrophages
    Authors: KM Krawczyk, H Nilsson, R Allaoui, D Lindgren, M Arvidsson, K Leandersso, ME Johansson
    Lab. Invest., 2017-07-31;97(11):1296-1305.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Expression and potential function of the CXC chemokine CXCL16 in pancreatic ductal adenocarcinoma.
    Authors: Wente MN, Gaida MM, Mayer C, Michalski CW, Haag N, Giese T, Felix K, Bergmann F, Giese NA, Friess H
    Int. J. Oncol., 2008-08-01;33(2):297-308.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Overexpression of CXCL16 and its receptor CXCR6/Bonzo promotes growth of human schwannomas.
    Authors: Held-Feindt J, Rehmke B, Mentlein R, Hattermann K, Knerlich F, Hugo HH, Ludwig A, Mehdorn HM
    Glia, 2008-05-01;56(7):764-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. High levels and inflammatory effects of soluble CXC ligand 16 (CXCL16) in coronary artery disease: down-regulatory effects of statins.
    Authors: Smith C, Halvorsen B, Otterdal K, Waehre T, Yndestad A, Fevang B, Sandberg WJ, Breland UM, Froland SS, Oie E, Gullestad L, Damas JK, Aukrust P
    Cardiovasc. Res., 2008-03-13;79(1):195-203.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Expression and role of CCR6/CCL20 chemokine axis in pulmonary sarcoidosis.
    Authors: Facco M, Baesso I, Miorin M, Bortoli M, Cabrelle A, Boscaro E, Gurrieri C, Trentin L, Zambello R, Calabrese F, Cassatella MA, Semenzato G, Agostini C
    J. Leukoc. Biol., 2007-07-05;82(4):946-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Novel antiviral activity of chemokines.
    Authors: Nakayama T, Shirane J, Hieshima K, Shibano M, Watanabe M, Jin Z, Nagakubo D, Saito T, Shimomura Y, Yoshie O
    Virology, 2006-04-17;350(2):484-92.
    Species: Virus
    Sample Types: Virus, Whole Cells
    Applications: Binding Assay, Bioassay

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human CXCL16 Protein

There are currently no reviews for this product. Be the first to review Recombinant Human CXCL16 Protein and earn rewards!

Have you used Recombinant Human CXCL16 Protein?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review