Recombinant Human Draxin Protein Summary
Product Specifications
When Recombinant Human Draxin is immobilized at 2 μg/mL, Recombinant Human LRP-6 Fc Chimera (Catalog # 1505-LR) binds with an apparent KD < 25 nM.
Also measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neuron.
Gly26-Val349, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
6148-DR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
6148-DR/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Draxin
Draxin (Dorsal repulsive axon guidance protein) also called neucrin (neural tissue-specific cysteine-rich protein) is a secreted, 58 kDa, presumably glycosylated member of the draxin family of repulsive guidance proteins (1 ‑ 5). In mammals, it is expressed in developing neurons (axons), astroglia, and likely cells of the developing somite (1 ‑ 3). Its expression appears to be limited to the brain and spinal cord (2, 4). Human Draxin mRNA encodes 349 amino acids (aa) that include a 25 aa signal sequence and a 324 aa mature, secreted protein containing one potential N-linked glycosylation site followed by a Cys-rich domain (aa 274 ‑ 333). The pattern of cysteines is similar to the second of two cysteine-rich regions in members of the DKK family of Wnt inhibitors (4). Mature human Draxin (aa 26 ‑ 349) shares 80%, 79%, 88% and 86% aa identity with mature mouse, rat, equine and bovine Draxin, respectively. Draxin is a repulsive guidance molecule that, like DKKs, acts as a Wnt antagonist by binding to LRP6 (4). Draxin is expressed by midline glial cells that act as intermediate guideposts for corpus callosum axons (1). Inactivation of the mouse Draxin gene results in lack of organization of axons into functional tracts or bundles (fasciculation), and failure of the corpus callosum, hippocampal and anterior commissures to form and cross the midline (1). Draxin-deficient mice also show abnormally small hippocampi, especially within the dentate gyrus, where excess apoptosis is detected during early postnatal life (3). In vitro, Draxin blocks migration of chick neural crest cells, while in vivo, ectopic over-expression inhibits growth of mouse axons or causes their misrouting (1, 2, 5).
- Islam, S.M. et al. (2009) Science 323:388.
- Su, Y. et al. (2009) Dev. Growth Differ. 51:787.
- Zhang, S. et al. (2010) Neurosci. Res. 66:53.
- Miyake, A. et al. (2009) Biochem. Biophys, Res. Commun. 390:1051.
- Naser, I.B. et al. (2009) Dev. Biol. 332:351.
Citation for Recombinant Human Draxin Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012;487(7408):505-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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