Recombinant Human Elongin B/Elongin C/VHL/CUL2/RBX1, CF

Catalog #: E3-655 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
E3-655-025

Recombinant Human Elongin B/Elongin C/VHL/CUL2/RBX1 SDS-PAGE

1 Image

All VHL/ELOB/ELOC/CUL2/RBX1 Complex Proteins and Enzymes

Product Details

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Elongin B/Elongin C/VHL/CUL2/RBX1, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Activity

Typical protein concentration for use in vitro will depend on experimental conditions.

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human VHL/ELOB/ELOC/CUL2/RBX1 Complex protein
Met231 - Asp443 (VHL), Met1 - Gln118 (ELOB), Met1 - Cys112 (ELOC), Met1 - Ala 745 with a N-terminal 10-His tag Sumo tag (CUL2), Met1 - His108 (RBX1)

Accession #

P40337.2, Q15370.1, Q15369.1, Q13617.2, P62877.1

Predicted Molecular Mass

25 kDa (VHL), 13.1 kDa (ELOB), 12.8 kDa (ELOC), 93 kDa (CUL2), 12 kDa (RBX1)

Product Datasheets

E3-655

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E3-655

Formulation	Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Scientific Data

SDS-PAGE

View Larger

1 μg and 2 μg per lane of Recombinant Human VHL/ELOB/ELOC/CUL2/RBX1 Complex (Catalog # E3-655) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing bands corresponding to the individual proteins of the complex.

Reconstitution Calculator

Background: VHL/ELOB/ELOC/CUL2/RBX1 Complex

VHL (von Hippel-Lindau disease tumor suppressor) is the substrate recognition subunit for an E3 ligase activity that ubiquitinates proteins containing hydroxyproline residues. Targets of VHL include HIF1 alpha, beta 2 adrenergic receptor, ZHX2 and others. In VHL-box E3 Ubiquitin ligases, VHL is bound to a heterodimer complex composed of Elongin B (ELOB) and Elongin C (ELOC) through the "BC-box motif" found in many proteins in the VHL-box and SOCS-box protein families. ELOB/ELOC serves as an adapter between substrate recognition proteins and Cullin-2 (or Cullin-5) and RBX1 to form an active E3 ligase.

References