Recombinant Human FCRL3/FcRH3 His-tag Protein, CF

Catalog # Availability Size / Price Qty
3126-FCB-050
Recombinant Human FCRL3/FcRH3 His-tag Protein Binding Activity.
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Citations (3)
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Recombinant Human FCRL3/FcRH3 His-tag Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human FCRL3/FcRH3 His-tag (Catalog # 3126-FCB) is immobilized at 2.00 μg/mL (100 μL/well), Normal Human IgG binds with an ED50 of 1.00-6.00 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human FCRL3/FcRH3 protein
Arg14-Arg569, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Arg14
Predicted Molecular Mass
62 kDa
SDS-PAGE
70-90 kDa, under reducing conditions.

Product Datasheets

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3126-FCB

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3126-FCB

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

Measured by its binding ability in a functional ELISA. When Recombinant Human FCRL3/FcRH3 His-tag Protein (Catalog # 3126-FCB) is immobilized at 2.00 μg/mL (100 μL/well), Normal Human IgG binds with an ED50 of 1.00‑6.00 μg/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Human FCRL3/FcRH3 His-tag Protein (Catalog # 3126-FCB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 70-90 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FCRL3/FcRH3

FCRL3 (Fc Receptor-Like 3), also known as FcRH3, IRTA3, and SPAP2, is a 110 kDa molecule with sequence homology to classical Fc receptors. The type 1 transmembrane FCRL proteins contain from three to nine immunoglobulin-like domains. They are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1). Mature human FCRL3 consists of a 556 amino acid (aa) extracellular domain (ECD) with six Ig-like domains, a 21 aa transmembrane segment, and a 140 aa cytoplasmic domain with four immunotyrosine inhibitory motifs (ITIMs) (2 - 4). Within the ECD, human and mouse FCRL3 share 35% aa sequence identity. Alternate splicing generates several additional isoforms with deletions or substitutions in both the extracellular and intracellular regions. These include potentially secreted forms that are truncated following the second Ig-like domain (4). FCRL3 is expressed in secondary lymphoid organs on the surface of mature naïve and memory B cells, NK cells, and B cell lines derived from chronic lymphocytic leukemias (2, 3, 5). It is upregulated on B cells following LPS or anti-CD40 stimulation (6). A polymorphism in the FCRL3 promoter induces enhanced transcription and is associated with the development of autoimmune disorders in a Japanese population (6, 7). Tyrosine phosphorylation within the ITIMs of FCRL3 enables its association with SHP-1 (4).

References
  1. Davis, R.S. (2007) Annu. Rev. Immunol. 25:525.
  2. Miller, I. et al. (2002) Blood, 99:2662.
  3. Davis, R.S. et al. (2001) Proc. Natl. Acad. Sci. 98:9772.
  4. Xu, M.-J. et al. (2002) Biochem. Biophys. Res. Commun. 293:1037.
  5. Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
  6. Kochi, Y. et al. (2005) Nat. Genet. 37:478.
  7. Chistiakov, D.A. and A.P. Chistiakov (2007) Hum. Immunol. 68:375.
Long Name
Fc Receptor-like 3
Entrez Gene IDs
115352 (Human); 329693 (Mouse); 680697 (Rat)
Alternate Names
CD307c; Fc receptor homolog 3; Fc receptor-like 3; Fc receptor-like protein 3; FcRH3; FCRL3; fcR-like protein 3; hIFGP3; IFGP3; immunoglobulin superfamily receptor translocation associated protein 3; IRTA3; SPAP2

Citations for Recombinant Human FCRL3/FcRH3 His-tag Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. MAIA, Fc receptor-like 3, supersedes JUNO as IZUMO1 receptor during human fertilization
    Authors: J Vondrakova, M Frolikova, L Ded, J Cerny, P Postlerova, V Palenikova, O Simonik, Z Nahacka, K Basus, E Valaskova, R Machan, A Pacey, Z Holubcova, P Koubek, Z Ezrova, S Park, R Liu, R Partha, N Clark, J Neuzil, M Ikawa, K Erickson, KS Lam, H Moore, K Komrskova
    Science Advances, 2022-09-07;8(36):eabn0047.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Human Fc Receptor-like 3 Inhibits Regulatory T Cell Function and Binds Secretory IgA
    Authors: S Agarwal, Z Kraus, J Dement-Bro, O Alabi, K Starost, M Tolnay
    Cell Rep, 2020-02-04;30(5):1292-1299.e3.
    Species: Human
    Sample Types: Protein, Recombinant Protein
    Applications: Surface Plasmon Resonance (SPR
  3. Human Fc receptor-like 5 binds intact IgG via mechanisms distinct from those of Fc receptors.
    Authors: Franco A, Damdinsuren B, Ise T, Dement-Brown J, Li H, Nagata S, Tolnay M
    J Immunol, 2013-04-24;190(11):5739-46.
    Species: Human
    Sample Types: Recombinant Proteins
    Applications: Surface Plasmon Resonance (SPR

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