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Recombinant Human GPVI Protein, CF

Catalog #: 3627-GP
1 Citation Datasheet / COA / SDS
His-tag
Catalog # Availability Size / Price Qty
3627-GP-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (1)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human GPVI Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When cross-linked with 10 µg/mL of cross-linking antibody Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050), rhGPVI will bind to Collagen I (1 µg/mL, 100 µL/well) with an apparent KD <40 nM.  Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human GPVI protein
Gln21-Lys267, with a C-terminal 6-His tag
Accession #
Q9HCN6
N-terminal Sequence
Analysis
No results obtained: Gln21 predicted
Predicted Molecular Mass
27.7 kDa
SDS-PAGE
50-55 kDa, reducing conditions

Product Datasheets

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3627-GP

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3627-GP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GPVI

Glycoprotein VI (GPVI) is a 63 kDa platelet/megakaryocyte-specific type I transmembrane glycoprotein of the immunoglobulin superfamily that is an important collagen receptor and initiator of platelet activation, aggregation and thrombin generation (1, 2). GPVI is also a secondary receptor required for platelet spreading on laminin (3). Human GPVI contains a 20 amino acid (aa) signal sequence, a 247 aa extracellular domain (ECD) that has two C-type Ig-like domains followed by a mucin-like, presumably O-glycosylated Ser-Thr-rich region, a 21 aa transmembrane (TM) domain and a 51 aa cytoplasmic tail that contains calmodulin-binding and SH3 domains. Human GPVI ECD shows 69%, 65% and 70% aa identity with mouse, bovine and canine GPVI ECD, respectively. Two splice variants exist; one is 17 aa shorter in the ECD, while the other diverges at aa 260, creating an inactive monomeric and presumably secreted 681 aa protein (3). GPVI associates with the Fc receptor gamma -chain via charged aa in the TM domains of GPVI (arginine) and the FcR gamma (aspartic acid) (2). Collagen binding by the GPVI Ig-like domains initiates signaling through the FcR gamma ITAM sequence (2). Dimerization of GPVI (2:2 with FcR gamma ) and N-glycosylation greatly enhances collagen binding (5, 6). Type I and III collagens are strong thrombus-forming components in the vascular subendothelium and atherosclerotic plaques (7). GPVI initiates binding to fibrillar collagens under flow conditions, then activates integrin alpha 2 beta 1 which binds collagen more tightly (8). GPVI deficiencies cause only a mild bleeding tendency, probably because integrin alpha 2 beta 1 is able to minimally initiate collagen binding (8). Normal human GPVI concentration can vary widely and affect maximum thrombin generation (9). Engagement of GPVI by collagens or other agonists, including autoantibodies, causes calmodulin-regulated metalloproteinase cleavage of the 57 kDa ECD and depletes surface GPVI (10).

References
  1. Jandrot-Perrus, M. et al. (2000) Blood 96:1798.
  2. Moroi, M. and S. M. Jung (2004) Thromb. Res. 114:221.
  3. Inoue, O. et al. (2006) Blood 107:1405.
  4. Ezumi, Y. et al. (2000) Biochem. Biophys. Res. Comm. 277:27.
  5. Horii, K. et al. (2006) Blood 108:936.
  6. Kunicki, T. J. et al. (2005) Blood 106:2744.
  7. Cosemans, J. M. et al. (2005) Atherosclerosis 181:19.
  8. Lecut, C. et al. (2005) Thromb. Haemost. 94:107.
  9. Furihata, K. et al. (2001) Arterioscler. Thromb. Vasc. Biol. 21:1857.
  10. Stephens, G. et al. (2005) Blood 105:186.
Long Name
Glycoprotein VI [Platelet]
Entrez Gene IDs
51206 (Human); 243816 (Mouse)
Alternate Names
Glycoprotein 6; glycoprotein VI (platelet); GP6; GPIV; GPVI; GPVIplatelet collagen receptor; MGC138168; platelet glycoprotein VI

Citation for Recombinant Human GPVI Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Assessing single-stranded oligonucleotide drug-induced effects in vitro reveals key risk factors for thrombocytopenia
    Authors: S Sewing, AB Roth, M Winter, A Dieckmann, C Bertinetti, Y Tessier, C McGinnis, S Huber, E Koller, C Ploix, JC Reed, T Singer, A Rothfuss
    PLoS ONE, 2017-11-06;12(11):e0187574.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay

FAQs

  1. This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?

    • The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.

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