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Recombinant Human HA-Ubiquitin Protein, CF

Catalog #: U-110
48 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
U-110-01M
R&D Systems Recombinant Proteins and Enzymes
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Citations (48)
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Reviews (2)
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Recombinant Human HA-Ubiquitin Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human HA-Ubiquitin can be conjugated to substrate proteins via the subsequent actions of a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human HA-Ubiquitin concentration of 5-50 μM.
Source
E. coli-derived human Ubiquitin protein
Met1 - Gly76 with N-terminal HA (YPYDVPDYA) tag
Accession #
P0CG47.1
Predicted Molecular Mass
9.8 kDa

Product Datasheets

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U-110

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

U-110

Formulation Supplied as a 0.2 μm filtered solution in HEPES.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ubiquitin

Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. Ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast Ubiquitin, and 100% aa sequence identity shared between human and mouse Ubiquitin (1). In mammals, four Ubiquitin genes encode for two Ubiquitin-ribosomal fusion proteins and two poly-Ubiquitin proteins. Cleavage of the Ubiquitin precursors by deubiquitinating enzymes gives rise to identical Ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of Ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of Ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, Ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1) (2,3). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting (4-7).


References
  1. Sharp, P.M. & W.-H. Li. (1987) Trends Ecol. Evol. 2:328.
  2. Ciechanover, A. et al. (1980 ) Proc. Natl. Acad. Sci. USA 77:1365.
  3. Hershko, A. et al. (1980) Proc. Natl. Acad. Sci. USA 77:1783.
  4. Greene, W. et al. (2012) PLoS Pathog. 8:e1002703.
  5. Tong, X. et al. (2012) J. Biol. Chem. 287:25280.
  6. Wei, W. et al. (2004) Nature 428:194.
  7. Wertz, I.E. et al. (2004) Nature 430:694.
  8. Coux O., et al. (1996) Ann. Rev. Biochem. 65: 801-847.
  9. Ellison M.J and Hochstrasser M. (1991) J. Biol. Chem. 266: 21150-21157.
  10. Finley D., et al. (1994) Mol. Cell. Biol. 14: 5501-5509.
  11. Fritze C.E. and Anderson T.R. (2000) Meth. Enz. 327: 3-16.
  12. Terpe K (2003) Appl. Microbiol. Biotech. 60: 523-533.
  13. Treier M., et al. (1994) Cell. 78: 787-798.
  14. Wilson I.A., et al. (1984) Cell 37:767-778.
Entrez Gene IDs
7314 (Human); 298693 (Rat)
Alternate Names
RPS27A; UBA52; UBB ubiquitin B; UBB; UBC; Ubiquitin

Citations for Recombinant Human HA-Ubiquitin Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

48 Citations: Showing 1 - 10
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  1. A dynamic ubiquitination balance of cell proliferation and endoreduplication regulators determines plant organ size
    Authors: Chen, Y;Vermeersch, M;Van Leene, J;De Jaeger, G;Li, Y;Vanhaeren, H;
    Science advances
    Species: Arcitic Ground Squirrel
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. The MYC2-PUB22-JAZ4 module plays a crucial role in jasmonate signaling in tomato
    Authors: Wu, S;Hu, C;Zhu, C;Fan, Y;Zhou, J;Xia, X;Shi, K;Zhou, Y;Foyer, CH;Yu, J;
    Molecular plant
    Species: olive baboon
    Sample Types: Recombinant Protein
    Applications: Bioassay
  3. Lenalidomide derivatives and proteolysis-targeting chimeras for controlling neosubstrate degradation
    Authors: Yamanaka, S;Furihata, H;Yanagihara, Y;Taya, A;Nagasaka, T;Usui, M;Nagaoka, K;Shoya, Y;Nishino, K;Yoshida, S;Kosako, H;Tanokura, M;Miyakawa, T;Imai, Y;Shibata, N;Sawasaki, T;
    Nature communications
    Applications: In vitro assay
  4. Reciprocal regulatory balance within the CLEC16A-RNF41 mitophagy complex depends on an intrinsically disordered protein region
    Authors: M Gingerich, J Zhu, B Chai, M Vincent, N Xie, V Sidarala, N Kotov, D Sahu, D Klionsky, S Schnell, S Soleimanpo
    The Journal of Biological Chemistry, 2023-02-22;0(0):103057.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  5. Induction of zinc finger protein RNF6 auto-ubiquitination for the treatment of myeloma and chronic myeloid leukemia
    Authors: H Zhuang, Y Ren, C Mao, Y Zhong, Z Zhang, B Cao, Y Zhang, J Huang, G Xu, Z Huang, Y Xu, X Mao
    The Journal of Biological Chemistry, 2022-08-01;298(9):102314.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  6. An engineered platform for reconstituting functional multisubunit SCF E3 ligase in vitro
    Authors: H Liu, S Liu, H Yu, X Huang, Y Wang, L Jiang, X Meng, G Liu, M Chen, Y Jing, F Yu, B Wang, J Li
    Molecular plant, 2022-06-24;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  7. BMI-1 regulates DNA end resection and homologous recombination repair
    Authors: A Fitieh, AJ Locke, F Mashayekhi, F Khaliqdina, AK Sharma, IH Ismail
    Cell Reports, 2022-03-22;38(12):110536.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  8. The E3 ligase TRAF4 Promotes IGF Signaling by Mediating Atypical Ubiquitination of IRS-1
    Authors: W Yu, R Singh, Z Wang, BW O'Malley, P Yi
    Oncogene, 2021-05-13;0(0):100739.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  9. DAZAP2 acts as specifier of the p53 response to DNA damage
    Authors: MC Liebl, J Moehlenbri, H Becker, G Raddatz, SK Abdeen, RI Aqeilan, F Lyko, TG Hofmann
    Nucleic Acids Research, 2021-03-18;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Ubiquitination
  10. Mutual regulation between OGT and XIAP to control colon cancer cell growth and invasion
    Authors: HG Seo, HB Kim, JY Yoon, TH Kweon, YS Park, J Kang, J Jung, S Son, EC Yi, TH Lee, WH Yang, JW Cho
    Cell Death Dis, 2020-09-29;11(9):815.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. The F-box protein FBXL16 up-regulates the stability of C-MYC oncoprotein by antagonizing the activity of the F-box protein FBW7
    Authors: M Morel, KN Shah, W Long
    J. Biol. Chem., 2020-04-28;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Ubiquitination
  12. CAND1 regulates lunapark for the proper tubular network of the endoplasmic reticulum
    Authors: H Kajiho, Y Yamamoto, T Sakisaka
    Sci Rep, 2019-09-11;9(1):13152.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  13. Impaired plasma membrane localization of ubiquitin ligase complex underlies 3-M syndrome development
    Authors: P Wang, F Yan, Z Li, Y Yu, SE Parnell, Y Xiong
    J. Clin. Invest., 2019-07-25;130(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  14. DNA requirement in FANCD2 deubiquitination by USP1-UAF1-RAD51AP1 in the Fanconi anemia DNA damage response
    Authors: F Liang, AS Miller, S Longerich, C Tang, D Maranon, EA Williamson, R Hromas, C Wiese, GM Kupfer, P Sung
    Nat Commun, 2019-06-28;10(1):2849.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  15. Autophagy induction in atrophic muscle cells requires ULK1 activation by TRIM32 through unanchored K63-linked polyubiquitin chains
    Authors: M Di Rienzo, M Antonioli, C Fusco, Y Liu, M Mari, I Orhon, G Refolo, F Germani, M Corazzari, A Romagnoli, F Ciccosanti, B Mandriani, MT Pellico, R De La Torr, H Ding, M Dentice, M Neri, A Ferlini, F Reggiori, M Kulesz-Mar, M Piacentini, G Merla, GM Fimia
    Sci Adv, 2019-05-08;5(5):eaau8857.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Ubiquitination
  16. Mechanism for recycling tRNAs on stalled ribosomes
    Authors: MCJ Yip, AFA Keszei, Q Feng, V Chu, MJ McKenna, S Shao
    Nat. Struct. Mol. Biol., 2019-04-22;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Ubiquitination
  17. The potato cyst nematode effector RHA1B is a ubiquitin ligase and uses two distinct mechanisms to suppress plant immune signaling
    Authors: J Kud, W Wang, R Gross, Y Fan, L Huang, Y Yuan, A Gray, A Duarte, JC Kuhl, A Caplan, A Goverse, Y Liu, LM Dandurand, F Xiao
    PLoS Pathog., 2019-04-12;15(4):e1007720.
    Applications: Ubiquitination
  18. Binding of FANCI-FANCD2 Complex to RNA and R-Loops Stimulates Robust FANCD2 Monoubiquitination
    Authors: Z Liang, F Liang, Y Teng, X Chen, J Liu, S Longerich, T Rao, AM Green, NB Collins, Y Xiong, L Lan, P Sung, GM Kupfer
    Cell Rep, 2019-01-15;26(3):564-572.e5.
    Applications: Bioassay
  19. RNF126 Quenches RNF168 Function in the DNA Damage Response
    Authors: L Zhang, Z Wang, R Shi, X Zhu, J Zhou, B Peng, X Xu
    Genomics Proteomics Bioinformatics, 2018-12-04;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  20. Legionella pneumophila inhibits immune signalling via MavC-mediated transglutaminase-induced ubiquitination of UBE2N
    Authors: N Gan, ES Nakayasu, PJ Hollenbeck, ZQ Luo
    Nat Microbiol, 2018-11-12;4(1):134-143.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  21. Chromatin assembly factor-1 (CAF-1) chaperone regulates Cse4 deposition into chromatin in budding yeast
    Authors: GS Hewawasam, K Dhatchinam, M Mattingly, C Seidel, JL Gerton
    Nucleic Acids Res., 2018-05-18;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  22. PCGF5 is required for neural differentiation of embryonic stem cells
    Authors: M Yao, X Zhou, J Zhou, S Gong, G Hu, J Li, K Huang, P Lai, G Shi, AP Hutchins, H Sun, H Wang, H Yao
    Nat Commun, 2018-05-15;9(1):1463.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  23. A Regulatory Module Controlling Homeostasis of a Plant Immune Kinase
    Authors: J Wang, LE Grubb, J Wang, X Liang, L Li, C Gao, M Ma, F Feng, M Li, L Li, X Zhang, F Yu, Q Xie, S Chen, C Zipfel, J Monaghan, JM Zhou
    Mol. Cell, 2018-01-18;0(0):.
    Applications: Bioassay
  24. Sec16A, a key protein in COPII vesicle formation, regulates the stability and localization of the novel ubiquitin ligase RNF183
    Authors: Y Wu, XP Guo, S Kanemoto, Y Maeoka, A Saito, R Asada, K Matsuhisa, Y Ohtake, K Imaizumi, M Kaneko
    PLoS ONE, 2018-01-04;13(1):e0190407.
    Applications: Bioassay
  25. Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis
    Authors: LT Gou, JY Kang, P Dai, X Wang, F Li, S Zhao, M Zhang, MM Hua, Y Lu, Y Zhu, Z Li, H Chen, LG Wu, D Li, XD Fu, J Li, HJ Shi, MF Liu
    Cell, 2017-05-25;0(0):.
    Applications: Bioassay
  26. CP5 system, for simple and highly efficient protein purification with a C-terminal designed mini tag
    Authors: H Takeda, W Zhou, K Kido, R Suno, T Iwasaki, T Kobayashi, T Sawasaki
    PLoS ONE, 2017-05-25;12(5):e0178246.
    Applications: Enzyme Assay
  27. Bam-dependent deubiquitinase complex can disrupt germ-line stem cell maintenance by targeting cyclin A
    Authors: S Ji, C Li, L Hu, K Liu, J Mei, Y Luo, Y Tao, Z Xia, Q Sun, D Chen
    Proc. Natl. Acad. Sci. U.S.A., 2017-05-08;0(0):.
    Species: Drosophila
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Smurf1 inhibits integrin activation by controlling Kindlin-2 ubiquitination and degradation
    Authors: X Wei, X Wang, J Zhan, Y Chen, W Fang, L Zhang, H Zhang
    J. Cell Biol., 2017-04-13;0(0):.
    Applications: Bioassay
  29. Epilepsy-associated gene Nedd4-2 mediates neuronal activity and seizure susceptibility through AMPA receptors
    Authors: J Zhu, KY Lee, KA Jewett, HY Man, HJ Chung, NP Tsai
    PLoS Genet, 2017-02-17;13(2):e1006634.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  30. Arabidopsis E3 ubiquitin ligase PLANT U-BOX13 (PUB13) regulates chitin receptor LYSIN MOTIF RECEPTOR KINASE5 (LYK5) protein abundance
    Authors: D Liao, Y Cao, X Sun, C Espinoza, CT Nguyen, Y Liang, G Stacey
    New Phytol, 2017-02-14;0(0):.
    Applications: Enzyme Assay
  31. The Nedd8 Non-covalent Binding Region in the Smurf HECT Domain is Critical to its Ubiquitn Ligase Function
    Authors: S He, Y Cao, P Xie, G Dong, L Zhang
    Sci Rep, 2017-02-07;7(0):41364.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  32. S.�pombe Uba1-Ubc15 Structure Reveals a Novel Regulatory Mechanism of Ubiquitin E2 Activity
    Authors: Z Lv, KA Rickman, L Yuan, K Williams, SP Selvam, AN Woosley, PH Howe, B Ogretmen, A Smogorzews, SK Olsen
    Mol. Cell, 2017-02-02;65(4):699-714.e6.
    Applications: Ubiquitination
  33. Mechanism of Ubiquitination and Deubiquitination in the Fanconi Anemia Pathway
    Authors: Sylvie van Twest
    Mol. Cell, 2016-12-13;0(0):.
    Applications: Bioassay
  34. SKP2 loss destabilizes EZH2 by promoting TRAF6-mediated ubiquitination to suppress prostate cancer
    Oncogene, 2016-11-21;0(0):.
    Applications: Bioassay
  35. Genome-wide identification and gene expression profiling of ubiquitin ligases for endoplasmic reticulum protein degradation
    Sci Rep, 2016-08-03;6(0):30955.
    Species: Human
    Sample Types: Protein
    Applications: Enzyme Assay
  36. The adenovirus E4-ORF3 protein functions as a SUMO E3 ligase for TIF-1? sumoylation and poly-SUMO chain elongation
    Proc Natl Acad Sci USA, 2016-05-31;113(24):6725-30.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  37. O-GlcNAcylation Antagonizes Phosphorylation of Cdh1 (CDC20 homologue 1)
    Authors: J Tian, Q Geng, Y Ding, J Liao, MQ Dong, X Xu, J Li
    J Biol Chem, 2016-04-14;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  38. Tumour suppressor TRIM33 targets nuclear beta-catenin degradation.
    Authors: Xue J, Chen Y, Wu Y, Wang Z, Zhou A, Zhang S, Lin K, Aldape K, Majumder S, Lu Z, Huang S
    Nat Commun, 2015-02-02;6(0):6156.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  39. Requirement of Smurf-mediated endocytosis of Patched1 in sonic hedgehog signal reception.
    Authors: Yue, Shen, Tang, Liu-Ya, Tang, Ying, Tang, Yi, Shen, Qiu-Hong, Ding, Jie, Chen, Yan, Zhang, Zengdi, Yu, Ting-Tin, Zhang, Ying E, Cheng, Steven Y
    Elife, 2014-06-12;3(0):.
    Applications: Bioassay
  40. A novel role for inhibitor of apoptosis (IAP) proteins as regulators of endothelial barrier function by mediating RhoA activation.
    Authors: Hornburger M, Mayer B, Leonhardt S, Willer E, Zahler S, Beyerle A, Rajalingam K, Vollmar A, Furst R
    FASEB J, 2013-12-26;28(4):1938-46.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Ubiquitination
  41. Parkin-catalyzed ubiquitin-ester transfer is triggered by PINK1-dependent phosphorylation.
    Authors: Iguchi, Masahiro, Kujuro, Yuki, Okatsu, Kei, Koyano, Fumika, Kosako, Hidetaka, Kimura, Mayumi, Suzuki, Norihiro, Uchiyama, Shinichi, Tanaka, Keiji, Matsuda, Noriyuki
    J Biol Chem, 2013-06-10;288(30):22019-32.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  42. C. elegans ring finger protein RNF-113 is involved in interstrand DNA crosslink repair and interacts with a RAD51C homolog.
    Authors: Lee, Hyojin, Alpi, Arno F, Park, Mi So, Rose, Ann, Koo, Hyeon-So
    PLoS ONE, 2013-03-28;8(3):e60071.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay
  43. ASH2L regulates ubiquitylation signaling to MLL: trans-regulation of H3 K4 methylation in higher eukaryotes.
    Authors: Wu L, Lee S, Zhou B, Nguyen U, Muir T, Tan S, Dou Y
    Mol Cell, 2013-02-28;49(6):1108-20.
    Applications: Bioassay
  44. Coordinated regulation of XPA stability by ATR and HERC2 during nucleotide excision repair.
    Authors: Lee, T-H, Park, J-M, Leem, S-H, Kang, T-H
    Oncogene, 2012-11-26;33(1):19-25.
    Applications: Bioassay
  45. A subset of mixed lineage leukemia proteins has plant homeodomain (PHD)-mediated E3 ligase activity.
    Authors: Wang, Jingya, Muntean, Andrew G, Wu, Laura, Hess, Jay L
    J Biol Chem, 2012-11-05;287(52):43410-6.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  46. High yield expression of catalytically active USP18 (UBP43) using a Trigger Factor fusion system.
    Authors: Basters A, Ketscher L, Deuerling E, Arkona C, Rademann J, Knobeloch K, Fritz G
    BMC Biotechnol, 2012-08-23;12(0):56.
    Applications: Bioassay
  47. TRIM16 acts as an E3 ubiquitin ligase and can heterodimerize with other TRIM family members.
    Authors: Bell JL, Malyukova A, Holien JK
    PLoS ONE, 2012-05-21;7(5):e37470.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  48. Functional analysis of NopM, a novel E3 ubiquitin ligase (NEL) domain effector of Rhizobium sp. strain NGR234.
    Authors: Xin DW, Liao S, Xie ZP
    PLoS Pathog., 2012-05-17;8(5):e1002707.

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Recombinant Human HA-Ubiquitin Protein, CF
By valentina rossio on 12/05/2018
Application: Enzymatic activity in vitro
Recombinant Human HA-Ubiquitin Protein, CF
By Anonymous on 05/30/2017
Application: Binding assay/Protein-protein interaction