Recombinant Human HGF (NS0-expressed) Biotin Protein, CF

Catalog #: BT294 Datasheet / COA / SDS

Biotinylated

Catalog #	Availability	Size / Price	Qty
BT294-025

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human HGF (NS0-expressed) Biotin Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Biotinylated Recombinant Human HGF Prorein (Catalog # BT294) is captured on EvenCoat Streptavidin Coated Plates (Catalog # CP004) at 0.500 μg/mL (100 μL/well), Recombinant Human HGFR/c-MET Fc Chimera Protein (Catalog # 8614-MT) binds with an ED₅₀ of 6.00‑60.0 ng/mL.

Source

Mouse myeloma cell line, NS0-derived human HGF protein
Gln32-Ser728 (Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile)

Accession #

P14210.2

N-terminal Sequence
Analysis

alpha chain: No results obtained; Gln32 predicted

beta chain: Val495

Structure / Form

Disulfide-linked heterodimer

Biotinylated via amines

Predicted Molecular Mass

alpha chain: 53.7 kDa

beta chain: 26 kDa

SDS-PAGE

55-69 kDa and 24-39 kDa, under reducing conditions.

Product Datasheets

BT294

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT294

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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Measured by its binding ability in a functional ELISA. When Biotinylated Recombinant Human HGF Protein (Catalog # BT294) is captured on EvenCoat Streptavidin Coated Plates (CP004) at 0.500 μg/mL (100 μL/well), Recombinant Human HGFR/c-MET Fc Chimera Protein (8614-MT) binds with an ED₅₀ of 6.00-60.0 ng/mL.

Reconstitution Calculator

Background: HGF

HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1-5). Human HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide-linked HGF with a 60 kDa alpha and 30 kDa beta chain. Alternate splicing generates human HGF isoforms that lack the proteinase-like domain and different numbers of the Kringle domains. Human HGF shares 91%-94% aa sequence identity with bovine, canine, feline, mouse, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF-dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the up-regulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (11). This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of nectin-1 alpha ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15).

References

Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40.
Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21.
Rosario, M. and W. Birchmeier (2004) Dev. Cell 7:3.
Lesk, A.M. and W.D. Fordham (1996) J. Mol. Biol. 258:501.
Nakamura, T. et al. (1989) Nature 342:440.
Mizuno, K. et al. (1994) J. Biol. Chem. 269:1131.
Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039.
Corso, S. et al. (2005) Trends Mol. Med. 11:284.
Maeshima, A. et al. (2000) Kid. Int. 58:1511.
Montesano, R. et al. (1991) Cell 67:901.
Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261.
Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531.
Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
Mineo, R. et al. (1994) Endocrinology 145:4355.
Urbanek, K. et al. (2005) Circ. Res. 97:663.

Long Name

Hepatocyte Growth Factor

Entrez Gene IDs

3082 (Human); 15234 (Mouse); 24446 (Rat); 403441 (Canine); 102133907 (Cynomolgus Monkey); 493705 (Feline)

Alternate Names

deafness, autosomal recessive 39; DFNB39; EC 3.4.21; EC 3.4.21.7; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; HGF; HGFB; HPTA; HPTAhepatocyte growth factor; lung fibroblast-derived mitogen; Scatter factor; SF; SFhepatopoeitin-A