Recombinant Human His6-alpha-Synuclein Protein, CF

Catalog #: SP-480 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
SP-480-500

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human His6-alpha-Synuclein Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Activity

Recombinant Human His6-alpha -Synuclein is ideal for use as a control substrate for in vitro Ubiquitin conjugation using select Ubiquitin E3 ligases such as CHIP/Stub1. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-alpha -Synuclein concentration of 0.5-2.5 μM.

Source

E. coli-derived human alpha-Synuclein protein
Met1 - Ala140 with a C-terminal 6-His tag

Accession #

P37840.1

Predicted Molecular Mass

15 kDa

Product Datasheets

SP-480

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

SP-480

Formulation	Supplied as a 0.2 μm filtered solution in HEPES and NaCl.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: alpha-Synuclein

alpha -Synuclein is member of a family of small soluble proteins that include also beta -, and gamma -Synuclein. It is predominantly expressed in neurons of the central nervous system in the presynaptic region of nerve terminals, where it cycles between a free, partially unfolded and a helical, membrane-bound form. alpha -Synuclein can self-aggregate in vivo and in vitro, forming various oligomeric species and fibrillar and amorphous aggregates. The fibrils and amyloidal forms of alpha -Synuclein are major components of Lewy bodies and Lewy neurites and have been linked to the pathogenesis of Parkinson’s Disease, Parkinson’s Disease Dementia, and dementia with Lewy bodies. alpha -Synuclein aggregates can be also found associated with amyloid plaques in Alzheirmer’s Disease.

References

Breydo L, et al. (2012) Biochim. Biophys. Acta. 1822: 261.
Chen R.H., et al. (2013) J. Biol. Chem. 288: 7438.
Li X., et al. (2008) Acta Biochim. Biophys. Sin (Shanghai) 40: 406.
Surguchov A. (2008) Int. Rev. Cell Mol. Biol. 270: 225.
Xia Q., et al. (2008) Front. Biosci. 13: 3850.

Entrez Gene IDs

6622 (Human); 20617 (Mouse); 29219 (Rat); 641350 (Porcine); 282857 (Bovine)

Alternate Names

alpha-Synuclein; I+/--synuclein; MGC110988; NACP; non A-beta component of AD amyloid; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; PARK1; PARK4; PD1; SNCA; synuclein alpha-140; synuclein, alpha (non A4 component of amyloid precursor); Synuclein-alpha; truncated alpha synuclein