Recombinant Human ICOS (aa 21-134) His-tag Protein, CF

Catalog #: 9865-CS Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9865-CS-050

Recombinant Human ICOS (aa 21-134) His-tag Protein Binding Activity

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Recombinant Human ICOS (aa 21-134) His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human B7-H2 Fc Chimera (Catalog # 165-B7) at 0.5 µg/mL (100 µL/well) can bind Recombinant Human ICOS with an ED₅₀ of 25-125 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human ICOS protein

Human ICOS (Glu21-Gln134) Accession # Q9Y6W8-1	HHHHHH	IEGR
N-terminus		C-terminus

Accession #

Q9Y6W8-1

N-terminal Sequence
Analysis

Glu21

Structure / Form

Noncovalent Homodimer

Predicted Molecular Mass

14 kDa

SDS-PAGE

20-34 kDa, reducing conditions

Product Datasheets

9865-CS

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9865-CS

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human B7-H2 Fc Chimera (Catalog # 165-B7) is immobilized at 0.5 µg/mL, Recombinant Human ICOS (Catalog # 9865-CS) binds with an ED₅₀ of 25-125 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human ICOS was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 20-34 kDa.

Reconstitution Calculator

Background: ICOS

Inducible co-stimulator (ICOS), also called AILIM (activation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors (1). Other family members are CD28, CTLA-4 and PD-1 (2, 4). Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% amino acid similarity with CD28 and CTLA-4. Human and mouse ICOS share approximately 72% amino acid identity. ICOS is expressed on most CD45RO+ cells (3). ICOS expression is up-regulated within approximately 24-48 hours of activation on Th primed cells (4, 6). B7-H2, a member of the B7 family of co-stimulatory ligands, has been identified as the ICOS ligand (4, 5). The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation (5, 7).

References

Aicher, A. et al. (2000) J. Immunol. 164:4689.
Coyle, A.J. et al. (2000) Immunity 13:95.
Coyle, A.J. and Gutierrez-Ramos, J.C. (2001) Nat. Immunol. 2:203.
Gonzalo, J.A. et al. (2001) J. Immunol. 166:1.
Hutloff, A. et al. (1999) Nature 397:263.
Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
Yoshinaga, S.K. et al. (1999) Nature 402:827.

Long Name

Inducible Co-stimulator

Entrez Gene IDs

29851 (Human); 54167 (Mouse); 102116512 (Cynomolgus Monkey)

Alternate Names

Activation-inducible lymphocyte immunomediatory molecule; AILIM; AILIMCD278; CD278 antigen; CD278; CRP-1; CVID1; ICOS; inducible costimulator; inducible T-cell costimulator; inducible T-cell co-stimulator; MGC39850