The specifications section on the website for Recombinant Human IL-17/IL-17A, Catalog # 7955-IL, describes SDS-PAGE as a range of Molecular Weights. Why?

Name: Recombinant Human IL-17A (Human Cell-expressed) Protein
Brand: R&D Systems
SKU: 7955-IL
Rating: 4.3 (3 reviews)

Recombinant Human IL-17/IL-17A, Catalog # 7955-IL, has one N-linked glycosylation site and seven O-linked glycosylation sites. The variable glycosylation results in multiple bands for this protein.

Recombinant Human IL-17A (Human Cell-expressed) Protein

Catalog #: 7955-IL
15 Citations 3 Reviews Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
7955-IL-025/CF
7955-IL-010/CF
7955-IL-100/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
7955-IL-025

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Recombinant Human IL-17A (Human Cell-expressed) Protein Bioactivity

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All IL-17/IL-17A Proteins and Enzymes

Product Details

Citations (15)

FAQs

Reviews (3)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-17A (Human Cell-expressed) Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells. The ED₅₀ for this effect is 0.12-1.2 ng/mL. Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. Yao, Z. et al. (1995) Immunity 3:811. The ED₅₀ for this effect is 1.5-7.5 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human IL-17/IL-17A protein
Gly24-Ala155

Accession #

Q16552

N-terminal Sequence
Analysis

Gly24

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

15.1 kDa (monomer)

SDS-PAGE

15-23 kDa, reducing conditions
28-38 kDa, non-reducing conditions

Product Datasheets

7955-IL (with carrier)

Product Datasheet

COA

SDS

7955-IL/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7955-IL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7955-IL/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Human IL-17 (Catalog # 7955-IL) induces CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells. The ED₅₀ for this effect is 0.12‑1.2 ng/mL.

Reconstitution Calculator

Background: IL-17/IL-17A

Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20 kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature human IL‑17A shares 60% amino acid sequence identity with mouse and rat IL‑17A (3, 4). IL‑17A is secreted by Th17 cells, gamma /δ T cells, iNKT cells, NK cells, LTi cells, neutrophils, and intestinal Paneth cells (2). It forms disulfide‑linked homodimers as well as disulfide‑linked heterodimers with IL‑17F (5, 6). IL‑17A exerts its effects through the transmembrane IL‑17 RA in complex with IL‑17 RC or IL‑17 RD (7, 8). Both IL‑17 RA and IL‑17 RC are required for responsiveness to heterodimeric IL‑17A/F (7). IL‑17A promotes protective mucosal and epidermal inflammation in response to microbial infection (9‑12). It induces chemokine production, neutrophil influx, and the production of antibacterial peptides (9‑11). IL‑17A/F likewise induces neutrophil migration, but IL‑17F does not (11). IL‑17A additionally enhances the production of inflammatory mediators by rheumatoid synovial fibroblasts and contributes to TNF‑ alpha induced shock (4, 13). In contrast, it can protect against the progression of colitis by limiting chronic inflammation (12). IL‑17A encourages the formation of autoreactive germinal centers and exacerbates the onset and progression of experimental models of autoimmunity (14, 15). IL‑17A has been shown to exert either tumorigenic or anti‑tumor effects (16, 17).

References

Gaffen, S.L. (2009) Nat. Rev. Immunol. 9:556.
Cua, D.J. and C.M. Tato (2010) Nat. Rev. Immunol. 10:479.
Yao, Z. et al. (1995) J. Immunol. 155:5483.
Fossiez, F. et al. (1996) J. Exp. Med. 183:2593.
Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
Wright, J.F. et al. (2008) J. Immunol. 181:2799.
Rong, Z. et al. (2009) Cell Res. 19:208.
Cho, J.S. et al. (2010) J. Clin. Invest. 120:1762.
Liang, S.C. et al. (2006) J. Exp. Med. 203:2271.
Liang, S.C. et al. (2007) J. Immunol. 179:7791.
O’Connor Jr., W. et al. (2009) Nat. Immunol. 10:603.
Takahashi, N. et al. (2008) J. Exp. Med. 205:1755.
Hsu, H. et al. (2008) Nat. Immunol. 9:166.
Rohn, T.A. et al. (2006) Eur. J. Immunol. 36:2857.
Wang, L. et al. (2009) J. Exp. Med. 206:1457.
Kryczek, I. et al. (2009) Blood 114:357.

Long Name

Interleukin 17

Entrez Gene IDs

3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)

Alternate Names

CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A

Related Research Areas

Citations for Recombinant Human IL-17A (Human Cell-expressed) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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TNF-? and Poly(I:C) induction of A20 and activation of NF-?B signaling are independent of ABCF1 in human airway epithelial cells
Authors: Cao, QT;Ishak, M;Shpilman, I;Hirota, JA;
Scientific reports
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Cytokine responsive networks in human colonic epithelial organoids unveil a molecular classification of inflammatory bowel disease
Authors: P Pavlidis, A Tsakmaki, A Treveil, K Li, D Cozzetto, F Yang, U Niazi, BH Hayee, M Saqi, J Friedman, T Korcsmaros, G Bewick, N Powell
Cell Reports, 2022-09-27;40(13):111439.
Species: Human
Sample Types: Organoid
Applications: Bioassay
Evidence for anti-inflammatory effects and modulation of neurotransmitter metabolism by Salvia officinalis L
Authors: G Margetts, S Kleidonas, NS Zaibi, MS Zaibi, KD Edwards
BMC complementary medicine and therapies, 2022-05-12;22(1):131.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
IL-17A Damages the Blood-Retinal Barrier through Activating the Janus Kinase 1 Pathway
Authors: EM Byrne, M Llorián-Sa, M Tang, A Margariti, M Chen, H Xu
Biomedicines, 2021-07-16;9(7):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Characterization of the interleukin-17 effect on articular cartilage in a translational model: an explorative study
Authors: D Sinkeviciu, A Aspberg, Y He, AC Bay-Jensen, P Önnerfjord
BMC Rheumatol, 2020-05-12;4(0):30.
Species: Bovine
Sample Types: Whole Tissue
Applications: Bioassay
IL-10 Dampens an IL-17-Mediated Periodontitis-Associated Inflammatory Network
Authors: L Sun, M Girnary, L Wang, Y Jiao, E Zeng, K Mercer, J Zhang, JT Marchesan, N Yu, K Moss, YL Lei, S Offenbache, S Zhang
J. Immunol., 2020-03-13;204(8):2177-2191.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
IL-36? regulates mediators of tissue homeostasis in epithelial cells
Authors: JE Heath, GM Scholz, PD Veith, EC Reynolds
Cytokine, 2019-03-08;119(0):24-31.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
IFN-? and IL-17A differentially influence the response of human macrophages and neutrophils to Pseudomonas aeruginosa infection
Authors: S Muntaka, Y Almuhanna, D Jackson, S Singh, A Afryie-Asa, M Cámara, L Martínez-P
Infect. Immun., 2019-01-24;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
The small molecule rhodomyrtone suppresses TNF-? and IL-17A-induced keratinocyte inflammatory responses: A potential new therapeutic for psoriasis
Authors: J Chorachoo, S Lambert, T Furnholm, L Roberts, L Reingold, S Auepemkiat, SP Voravuthik, A Johnston
PLoS ONE, 2018-10-15;13(10):e0205340.
Species: Human
Sample Types: Whole Tissue
Applications: Bioassay
Prolonging culture of primary human keratinocytes isolated from suction blisters with the Rho kinase inhibitor Y-27632
Authors: ED Anderson, I Sastalla, NJ Earland, M Mahnaz, IN Moore, F Otaizo-Car, TG Myers, CA Myles, SK Datta, IA Myles
PLoS ONE, 2018-09-12;13(9):e0198862.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Electrophilic properties of itaconate and derivatives regulate theï¿½I?B?-ATF3 inflammatory axis
Authors: M Bambouskov, L Gorvel, V Lampropoul, A Sergushich, E Loginichev, K Johnson, D Korenfeld, ME Mathyer, H Kim, LH Huang, D Duncan, H Bregman, A Keskin, A Santeford, RS Apte, R Sehgal, B Johnson, GK Amarasingh, MP Soares, T Satoh, S Akira, T Hai, C de Guzman, K Auclair, TP Roddy, SA Biller, M Jovanovic, E Klechevsky, KM Stewart, GJ Randolph, MN Artyomov
Nature, 2018-04-18;556(7702):501-504.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Intramuscular vaccination of guinea pigs with the live-attenuated human herpes simplex vaccine VC2 stimulates a transcriptional profile of vaginal Th17 and regulatory Tr1 responses
Authors: BA Stanfield, PJF Rider, J Caskey, F Del Piero, KG Kousoulas
Vaccine, 2018-04-11;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

Population snapshots predict early haematopoietic and erythroid hierarchies
Authors: BK Tusi, SL Wolock, C Weinreb, Y Hwang, D Hidalgo, R Zilionis, A Waisman, JR Huh, AM Klein, M Socolovsky
Nature, 2018-02-21;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

RNA-Seq Analysis of IL-1B and IL-36 Responses in Epidermal Keratinocytes Identifies a Shared MyD88-Dependent Gene Signature
Authors: WR Swindell, MA Beamer, MK Sarkar, S Loftus, J Fullmer, X Xing, NL Ward, LC Tsoi, MJ Kahlenberg, Y Liang, JE Gudjonsson
Front Immunol, 2018-01-29;9(0):80.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

Impaired Tight Junctions in Atopic Dermatitis Skin and in a Skin-Equivalent Model Treated with Interleukin-17
PLoS ONE, 2016-09-02;11(9):e0161759.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

FAQs

The specifications section on the website for Recombinant Human IL-17/IL-17A, Catalog # 7955-IL, describes SDS-PAGE as a range of Molecular Weights. Why?
- Recombinant Human IL-17/IL-17A, Catalog # 7955-IL, has one N-linked glycosylation site and seven O-linked glycosylation sites. The variable glycosylation results in multiple bands for this protein.

View all Proteins and Enzyme FAQs